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Method for fermenting temperature-sensitive strains by phosphoric acid to produce glutamic acid

A glutamic acid and temperature-sensitive technology, which is used in the field of temperature-sensitive strains to ferment glutamic acid and phosphoric acid, can solve the problems of uneven mixing of fermentation medium, shorten the preparation time of fermentation medium, etc., so as to improve acid production. rate and sugar-acid conversion rate, shortening the preparation time, shortening the effect of the fermentation cycle

Inactive Publication Date: 2017-05-24
COFCO BIO CHEM ENERGY LONGJIANG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to overcome the deficiencies of the prior art, and provide a method for using phosphoric acid to ferment and produce glutamic acid by thermosensitive strains. In this method, phosphoric acid is used instead of thermosensitive strains to ferment and produce glutamic acid The phosphate in the fermentation medium is prepared to produce glutamic acid by fermentation. On the one hand, it can overcome the long time required for phosphate to be completely dissolved in water, which may easily cause uneven mixing of the fermentation medium, shorten the preparation time of the fermentation medium, and improve Productivity; on the other hand, it can increase the specific acid production rate, shorten the fermentation cycle, and promote the acid production of bacteria, thereby reducing production costs, increasing the acid production rate and sugar-acid conversion rate

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0047] A method for using phosphoric acid to ferment and produce glutamic acid by thermosensitive strains, the process steps and conditions of its fermentation culture are as follows:

[0048] (1) Ingredients: According to the proportioning ratio, weigh various raw materials well and dissolve them completely to make 6L fermentation medium;

[0049] The raw material ratio of the fermentation medium is as follows: hydrolyzed sugar 55-60g / L, corn steep liquor 20-25g / L, molasses 15-18g / L, soybean meal hydrolyzate 1.0-2.1g / L, H 3 PO4 0.8~3g / L, MgSO4 7H2O 1~2.1g / L, MnSO4 7H2O 0.008~0.01g / L, FeSO4 7H2O 0.055~0.085g / L, KCl 5~7g / L, threonine 0.08 ~0.15g / L, antifoaming agent 0.031~0.035g / L, neutralized with sodium hydroxide to adjust the pH of the fermentation medium to 6.8~7.0.

[0050] (2) Sterilization: Sterilize the prepared fermentation medium at a temperature of 108-110°C for 8-10 minutes, cool the sterilized medium to 33°C in the fermentation and wait for inoculation;

[0051] ...

Embodiment 2

[0057] A method for using phosphoric acid to ferment and produce glutamic acid by thermosensitive strains, the process steps and conditions of its fermentation culture are as follows:

[0058] (1) Ingredients: According to the proportioning ratio, weigh various raw materials well and dissolve them completely to make 6L fermentation medium;

[0059] The raw material ratio of the fermentation medium is as follows: hydrolyzed sugar 55-60g / L, corn steep liquor 20-25g / L, molasses 15-18g / L, soybean meal hydrolyzate 1.0-2.1g / L, H 3 PO4 0.8~3g / L, MgSO4 7H2O 1~2.1g / L, MnSO4 7H2O 0.008~0.01g / L, FeSO4 7H2O 0.055~0.085g / L, KCl 5~7g / L, threonine 0.08 ~0.15g / L, defoamer 0.031~0.035g / L, neutralized with sodium carbonate to adjust the pH of the fermentation medium to 6.8~7.0.

[0060] (2) Sterilization: Sterilize the prepared fermentation medium at a temperature of 108-110°C for 8-10 minutes, cool the sterilized medium to 33°C in the fermentation and wait for inoculation;

[0061] (3) the s...

Embodiment 3

[0067] A method for using phosphoric acid to ferment and produce glutamic acid by thermosensitive strains, the process steps and conditions of its fermentation culture are as follows:

[0068] (1) Ingredients: According to the proportioning ratio, weigh various raw materials well and dissolve them completely to make 6L fermentation medium;

[0069] The raw material ratio of the fermentation medium is as follows: hydrolyzed sugar 50-65g / L, corn steep liquor 15-30g / L, molasses 10-18g / L, soybean meal hydrolyzate 1.0-2.1g / L, H 3 PO 4 0.8~4g / L, MgSO 4 ·7H 2 O 1.0~2.1g / L, MnSO 4 ·7H 2 O0.064~0.01g / L, FeSO 4 ·7H 2 O 0.055~0.085g / L, KCl 2~7g / L, threonine 0.08~0.15g / L, defoamer 0.031~0.035g / L, use ammonia water to neutralize phosphoric acid to make the pH of the fermentation medium 6.5~7.2 .

[0070] (2) Sterilization: Sterilize the prepared fermentation medium at a temperature of 108-110°C for 8-10 minutes, cool the sterilized medium to 33°C in the fermentation and wait for in...

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Abstract

The invention relates to a method for fermenting temperature-sensitive strains by phosphoric acid to produce glutamic acid. In a fermentation production process, the phosphoric acid is used for replacing phosphate in a process for fermenting the temperature-sensitive strains to produce the glutamic acid to prepare a fermentation culture medium; the glutamic acid is produced through fermentation. In the fermentation process of the method provided by the invention, the equal mol equivalent of the phosphoric acid is used for replacing the phosphate of the fermentation culture medium, and then a pH (Potential of Hydrogen) value of fermentation culture is neutralized by utilizing an alkaline substance; on one hand, the disadvantages that the time for completely dissolving the phosphate into water is long so that the fermentation culture medium is not uniformly mixed easily can be overcome, the preparation time of the fermentation culture medium is shortened and the production efficiency is improved; on the other hand, the acid production speed can be improved, a fermentation period is shortened and acid production of thalli is facilitated, so that the production cost is reduced; the acid production ratio and the saccharic acid invert ratio are improved; finally, the acid production level is also remarkably improved and can be improved by about 15 percent; the yield of the glutamic acid and the saccharic acid invert ratio are improved; the method has a wide industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering, in particular to a method for using phosphoric acid to ferment and produce glutamic acid by thermosensitive strains. Background technique [0002] Inorganic salts are indispensable substances for microbial life activities. Phosphorus, potassium and other nutrients are needed in the fermentation process of glutamic acid. Phosphorus is the main element of oxidative phosphorylation in the metabolic process of inorganic salts. It is also a component of nucleic acids, phospholipids, and certain coenzymes; high-energy phosphate bonds play an important role in energy storage and transmission. Phosphate is also an important buffering agent. It is no exception in glutamic acid fermentation. The level of phosphorus content has a great influence on it. If the concentration of phosphorus is too high, the metabolism of the bacteria tends to turn to the synthesis of xetine. If the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/14
CPCC12P13/14
Inventor 佟毅韩隽李辉王林曹宝泉王振王胜
Owner COFCO BIO CHEM ENERGY LONGJIANG CO LTD
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