Preserving stationary liquid for pathological tissues
A fixative and tissue technology, applied in the field of specimen processing, can solve the problems that the composition of the processing liquid is not easy to control, and its properties are greatly affected by human and environmental factors, so as to prevent tissue and cell autolysis, have no toxic side effects, and have good toughness Effect
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Embodiment 1
[0018] A pathological tissue preservation solution, which is composed of: menthol 13g, glycerin 14g, ethoxylated acetylene glycol 7ml, chlorogenic acid 13g, polyvinylpyrrolidone 40g, sodium chloride 32g, pure water to 1000ml.
[0019] The preparation method of the preservation fixative of the above-mentioned pathological tissues is as follows: take respectively menthol, glycerin, ethoxylated acetylene glycol, chlorogenic acid, polyvinylpyrrolidone and sodium chloride of the above-mentioned weight; Completely dissolve pure water to obtain sodium chloride solution; then add glycerin, menthol, chlorogenic acid and ethoxylated acetylenediol in sequence, and stir and mix for 12 minutes; finally add polyvinylpyrrolidone, and add pure water while stirring When the volume of the mixture is 1000ml, continue to stir at 2000rpm / min until the state of the mixture is uniform.
[0020] In order to understand the application of the pathological tissue preservation fixative of the present inv...
Embodiment 2
[0022] A pathological tissue preservation solution consists of: menthol 14g, glycerin 16g, ethoxylated acetylene glycol 8ml, chlorogenic acid 15g, polyvinylpyrrolidone 50g, sodium chloride 35g, pure water to 1000ml.
[0023] The preparation method of the preservation fixative of the above-mentioned pathological tissues is as follows: take respectively menthol, glycerin, ethoxylated acetylene glycol, chlorogenic acid, polyvinylpyrrolidone and sodium chloride of the above-mentioned weight; Completely dissolve pure water to obtain sodium chloride solution; then add glycerin, menthol, chlorogenic acid and ethoxylated acetylenediol in sequence, stir and mix for 15 minutes; finally add polyvinylpyrrolidone, and add pure water while stirring When the volume of the mixture is 1000ml, continue to stir at 2500rpm / min until the state of the mixture is uniform.
Embodiment 3
[0025] A pathological tissue preservation solution consists of: menthol 12g, glycerin 10g, ethoxylated acetylene glycol 5ml, chlorogenic acid 10g, polyvinylpyrrolidone 30g, sodium chloride 30g, pure water to 1000ml.
[0026] The preparation method of the preservation fixative of the above-mentioned pathological tissues is as follows: take respectively menthol, glycerin, ethoxylated acetylene glycol, chlorogenic acid, polyvinylpyrrolidone and sodium chloride of the above-mentioned weight; Completely dissolve pure water to obtain sodium chloride solution; then add glycerin, menthol, chlorogenic acid and ethoxylated acetylenediol in sequence, and stir and mix for 10 minutes; finally add polyvinylpyrrolidone, and add pure water while stirring When the volume of the mixture is 1000ml, continue stirring at 3000rpm / min until the state of the mixture is uniform.
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