Method for efficiently digesting antinutritional factors and antigen proteins in animal-breeding soybean meal by acetic acid method
An anti-nutritional factor and antigenic protein technology is applied in the field of efficient digestion of animal breeding anti-nutritional factors and antigenic proteins in soybean meal by acetic acid method to achieve the effect of improving nutritional quality and utilization value and efficient digestion
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Embodiment 1
[0018] Add 40g of soybean meal (dry weight) into a 1000mL stainless steel tank reactor, add 10% acetic acid at a solid-to-liquid ratio of 1:5, place in an electric heating constant temperature oil tank, and catalyze at 120°C for 30min. After drying, the soybean meal product catalyzed by acetic acid is obtained. It was found by SDS-PAGE that glycinin and β-conglycinin were completely digested; at the same time, trypsin inhibitor was reduced from 9.504mg / g to 0.341mg / g, which was 96.41%.
Embodiment 2
[0020] Add 1 kg of soybean meal (dry weight) into a 15L rotary cooker, add 10% acetic acid at a solid-to-liquid ratio of 1:5, and catalyze at 150° C. for 10 minutes to obtain the treated material. Evaporate and condense the acetic acid-water azeotrope contained in the processed material by vacuum evaporation, recover more than 92% of acetic acid, add an appropriate amount of powdered calcium carbonate to neutralize the material to a pH of 4.5-6.0, and dry it to obtain Digested soybean meal products. It was found by SDS-PAGE that glycinin and β-conglycinin were completely digested; at the same time, trypsin inhibitor was reduced from 9.504mg / g to 0.309mg / g, which was 96.75%.
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