Detoxification and rapid propagation method of sweet potatoes of Yizi 138
A sweet potato virus and sweet potato technology, which is applied in the field of sweet potato detoxification, can solve the problems of insignificant detoxification effect, influence on income, and degeneration of species, and achieve the effect of good detoxification effect and improvement of planting income.
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Embodiment 1
[0020] A virus-free and rapid propagation method of Yizi 138 sweet potato, the specific steps are:
[0021] (1) Obtaining of sterile explants:
[0022] Select the top 1.5cm-long stem tip of Yizi 138 sweet potato seedlings, soak it in 70% alcohol for 10 seconds, then disinfect it with 0.1% mercuric chloride for 8 minutes, then rinse it with sterile water 8 times, and put it on the ultra-clean workbench. Above, the shoot apex was peeled off under observation with a dissecting microscope, and a 0.3mm shoot apex meristem was obtained;
[0023] (2) shoot apical meristem culture detoxification:
[0024] Inoculate the shoot apex meristem obtained by stripping on the shoot apex induction medium, and place it under the condition that the temperature is 30°C and the light intensity is 3000lx, and carry out 18h light culture every day for 18 days, and transfer it into On the common culture medium, carry out 15h light culture every day, the culture time is 60 days, obtain the test-tube ...
Embodiment 2
[0034] A virus-free and rapid propagation method of Yizi 138 sweet potato, the specific steps are:
[0035] (1) Obtaining of sterile explants:
[0036] Select the top 1.5cm-long stem tip of Yizi 138 sweet potato seedlings, soak it in 70% alcohol for 15 seconds, then disinfect it with 0.1% mercury chloride for 6 minutes, then rinse it with sterile water for 6 times, and put it on the ultra-clean workbench. Above, the shoot apex was peeled off under observation with a dissecting microscope, and a 0.4mm shoot apex meristem was obtained;
[0037] (2) shoot apical meristem culture detoxification:
[0038] Inoculate the shoot apex meristem obtained by stripping on the shoot apex induction medium, place it still under the condition that the temperature is 30°C and the light intensity is 3000lx, and carry out 18h light culture every day for 25 days, and transfer it into On the common culture medium, carry out 15h light culture every day, the culture time is 55 days, obtain the test-...
Embodiment 3
[0048] A virus-free and rapid propagation method of Yizi 138 sweet potato, the specific steps are:
[0049] (1) Obtaining of sterile explants:
[0050] Select the top 1.5cm-long stem tip of Yizi 138 sweet potato seedlings, soak it in 70% alcohol for 13s, then disinfect it with 0.1% mercuric chloride for 7 minutes, then rinse it with sterile water for 7 times, and put it on the ultra-clean workbench. Above, the shoot apex was peeled off under observation with a dissecting microscope, and a 0.3mm shoot apex meristem was obtained;
[0051] (2) shoot apical meristem culture detoxification:
[0052] Inoculate the shoot apex meristem obtained by stripping on the shoot apex induction medium, and place it under the condition that the temperature is 30°C and the light intensity is 3000lx, and carry out 18h light culture every day for 22 days, and transfer it into On the common culture medium, carry out 15h light culture every day, the culture time is 57 days, obtain the test-tube pla...
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