A kind of open culture method and application of Pseudomonas stutzeri fstb-5
A technology of FSTB-5 and Pseudomonas stutzeri, which is applied in the field of environmental microorganisms, can solve the problems of unclean treatment, affecting the treatment effect, troublesome filtration methods, etc., and achieves reduced cultivation costs, good treatment effects, and salt tolerance strong effect
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Embodiment 1
[0024] The open culture method of Pseudomonas stutzeri FSTB-5 of the present invention comprises five stages of bacterial strain activation, liquid seed solution cultivation, aeration cultivation, pure bacterial strain detection and dominant bacterial strain maintenance, and specific process is as follows:
[0025] (1) Strain activation: Pseudomonas stutzeri FSTB-5 was inoculated on a plate containing FSTB solid medium, cultured at 35°C for 48 hours, and then stored in a refrigerator at 4°C until use. The FSTB solid medium is: FeSO 4 •7H 2 O 25mg / L, NH 4 NO 3 286mg / L, KCl 929mg / L, CaCl 2 2769mg / L, NaCl 21008mg / L, beef extract 5g / L, peptone 10g / L, agar 20g / L, pH 7.8.
[0026] (2) Liquid seed liquid culture: Prepare FSTB liquid medium, pack it into Erlenmeyer flasks, sterilize and cool to room temperature, use an inoculation loop to pick the activated strains in the slope and inoculate them into the Erlenmeyer flasks under a sterile environment. Incubate at 35°C for 48 ho...
Embodiment 2
[0031] The treatment process and operating conditions are the same as in Example 1, the difference is that in step (4) add rifamycin SV at a concentration of 5mg / L, and continue to add 5 culture cycles, and the floating microorganisms are drained and discharged Time to discharge, after which the normal open culture operation process is resumed. Collect the above cultured bacterial solution, spread it on a plate containing FSTB solid medium after dilution, count the grown colonies and count the percentage of colonies similar to FSTB-5.
Embodiment 3
[0033] The treatment process and operating conditions are the same as in Example 1, the difference is that in step (4), minocycline is added at a concentration of 25mg / L, and the addition is continued for 5 culture cycles. Ejected, after which the normal open culture operation process was resumed. Collect the above cultured bacterial solution, spread it on a plate containing FSTB solid medium after dilution, count the grown colonies and count the percentage of colonies similar to FSTB-5.
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