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Method for sterilizing tooth sample

A disinfection method and tooth technology, applied in the biological field, can solve problems such as unsatisfactory activity of dental pulp stem cells, and achieve the effect of avoiding bacterial contamination and high activity

Inactive Publication Date: 2017-05-10
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Although the technical content disclosed in the above two patents may be helpful in the disinfection of dental specimens, it has been found through actual experimental research that the disinfection effect and the activity of dental pulp stem cells after disinfection are not ideal.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: disinfection method of the present invention

[0034] 1. Preparing the preservation solution of the present invention

[0035] 8g sodium chloride (NaCl), 0.2g potassium chloride (KCl), 1.44g disodium hydrogen phosphate (NaCl 2 HPO 4 ), 0.24g potassium dihydrogen phosphate (KH 2 PO 4 ), adjust the pH to 7.2, and set the volume to 1L; then sterilize by high-pressure steam, take 10mL of the solution and add penicillin to make the final concentration 100U / mL, add streptomycin to make the final concentration 100μg / mL, add amphotericin B, Make the final concentration 1 μg / ml, and store at 4°C for later use.

[0036] 2. Disinfection method of the present invention

[0037] After the tooth specimens are collected, put them into a sterilized container filled with pre-cooled preservation solution for 10min-48h (according to the specific adjustment of the specimen delivery time). Then use sterile tweezers to take out the tooth specimen and put it in 75% ethanol...

Embodiment 2

[0040] Embodiment 2: disinfection method of the present invention

[0041] 1. Prepare the preservation solution of the present invention

[0042] 8g sodium chloride (NaCl), 0.2g potassium chloride (KCl), 1.44g disodium hydrogen phosphate (NaCl 2 HPO 4 ), 0.24g potassium dihydrogen phosphate (KH 2 PO 4 ), adjust the pH to 7.2, and set the volume to 1L; then autoclave, take 10mL of the solution and add penicillin to make the final concentration 50U / mL, add streptomycin to make the final concentration 500μg / mL, add amphotericin B, Make the final concentration 0.5 μg / ml, and store it at 4°C for later use.

[0043] 2, disinfection method of the present invention

[0044] After the tooth specimens are collected, put them into a sterilized container filled with pre-cooled preservation solution for 10min-48h (according to the specific adjustment of the specimen delivery time). Then use sterile tweezers to take out the tooth specimen and put it in iodophor disinfectant. If the to...

Embodiment 3

[0047] Embodiment 3: disinfection method of the present invention

[0048] 1. Preparing the preservation solution of the present invention

[0049] 8g sodium chloride (NaCl), 0.2g potassium chloride (KCl), 1.44g disodium hydrogen phosphate (NaCl 2 HPO 4 ), 0.24g potassium dihydrogen phosphate (KH 2 PO 4 ), adjust the pH to 7.2, and set the volume to 1L; then sterilize by high-pressure steam, take 10mL of the solution and add penicillin to make the final concentration 500U / mL, add streptomycin to make the final concentration 50μg / mL, add amphotericin B, Make the final concentration 5 μg / ml, and store it at 4°C for later use.

[0050] 2. Disinfection method of the present invention

[0051] After the tooth specimens are collected, put them into a sterilized container filled with pre-cooled preservation solution for 10min-48h (according to the specific adjustment of the specimen delivery time). Then use sterile tweezers to take out the tooth specimen and put it in iodophor ...

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PUM

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Abstract

The invention relates to the technical field of biology and discloses a method for sterilizing a tooth sample. The method includes: placing the pulled-out tooth sample in precooled preservation liquid, taking out the tooth sample for sterilizing, removing the periodontal tissue on the surface of the tooth, sterilizing again, and cleaning the tooth sample in the preservation liquid, wherein the preservation liquid comprises sodium chloride, potassium chloride, disodium hydrogen phosphate, potassium dihydrogen phosphate, penicillin, streptomycin and amphotericin B. The method has the advantages that the tooth sample preservation liquid is used for sterilizing in the sterilizing process, sterilizing is performed again after the periodontal tissue is removed, contamination of the sterilized tooth sample can be avoided, and extracted dental pulp stem cells still keep high activity and are not suffered from evident influence.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a tooth specimen disinfection method. Background technique [0002] Dental pulp stem cells (DPSCs) are a kind of mesenchymal stem cells with self-renewal, strong proliferation ability and multi-lineage differentiation ability existing in dental pulp tissue. Dental pulp stem cells have the potential of multi-directional differentiation. In addition to cells capable of forming mineralized nodules, they can also differentiate into fat, bone, cartilage, muscle, vascular endothelial, liver, nerve and other cells after being induced by different cytokines Department type. [0003] To successfully extract a sufficient amount of standard dental pulp stem cells from isolated teeth, the first problem encountered is the problem of disinfection of isolated teeth. The oral environment is different from other tissue environments. There are a large number of bacteria in the oral cavity, and the t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L2/18A61L101/48A61L101/34A01N1/02
CPCA01N1/0215A61L2/18
Inventor 葛啸虎陈海佳王一飞冯德龙
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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