Long-chain inulin regulates acute pancreatic inflammation and its associated tissue damage

A technology of acute pancreatitis and inulin, which is applied in the field of long-chain inulin to regulate acute pancreatic inflammation and related tissue damage caused by it, can solve the problems of different therapeutic intervention strategies and no protective effect on acute pancreatitis.

Active Publication Date: 2020-08-04
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The causes and locations of acute pancreatitis and colitis are different, and their therapeutic intervention strategies are also different. For example, butyric acid and Clostridium butyricum can alleviate colitis, but have no protective effect on acute pancreatitis

Method used

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  • Long-chain inulin regulates acute pancreatic inflammation and its associated tissue damage
  • Long-chain inulin regulates acute pancreatic inflammation and its associated tissue damage
  • Long-chain inulin regulates acute pancreatic inflammation and its associated tissue damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1. Establishment of acute pancreatitis model

[0036]Eight-week-old female BALB / C mice were randomly divided into a control group (control), an acute pancreatitis group (AP), and a long-chain inulin prevention group (ITF IV). The mice in the group and the prevention group were injected with normal saline (0.90%) and cerulein (50ug / kg); injected once every hour for 8 consecutive injections. During the experimental period, the mice in the three groups were fed the same diet, except that the prevention group ingested 5% (mass of long-chain inulin g / total weight of common mouse feed g) long-chain inulin feed for three consecutive days before injection of cerulein. . Long-chain inulin was purchased from the Netherlands sensus company.

Embodiment 2

[0037] Example 2. Long-chain inulin resists the expression of MPO in pancreatic tissue when acute pancreatitis occurs

[0038] As an important part of the pathogenesis of acute pancreatitis, the activation of white blood cells, especially neutrophils, plays an extremely important role in its pathological process. MPO is a functional marker and activation marker of neutrophils, and its level and activity changes represent the functional and active state of neutrophil polymorphonuclear leukocytes. Myeloperoxidase exists in neutrophils, and the amount of enzyme contained in each cell is certain, accounting for about 5% of the dry weight of the cell. This enzyme has the ability to reduce hydrogen peroxide. Using this feature, it can be analyzed Enzyme activity, and determination of the number of central leukocytes. The principle is as follows:

[0039] MPO+H 2 o 2 → complex; complex + AH 2 (hydrogen donor)→H 2 O+MPO+A product

[0040] The yellow compound is generated after ...

Embodiment 3

[0041] Example 3. Long-chain inulin reduces the level of serum amylase (AMY) when acute pancreatitis occurs

[0042] Existing data show that more than 90% of patients with acute pancreatitis have elevated serum AMY levels, so the present invention detects changes in AMY levels.

[0043] Amylase can hydrolyze starch to produce glucose, maltose and dextrin. When the substrate concentration is known and excessive, add iodine solution and unhydrolyzed starch to form a blue complex. According to the depth of blue, the hydrolyzed starch can be deduced amount, thus calculating the vitality of AMY. Such as figure 1 B, The results showed that the AMY activity of the acute pancreatitis group was significantly higher than that of the normal group, and the AMY activity of the long-chain inulin treatment group was significantly lower than that of the disease group. It can be concluded that the activity of AMY in mice with acute pancreatitis was significantly down-regulated after the prev...

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PUM

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Abstract

The invention discloses the effect of long-chain inulin on regulating acute pancreas inflammation and related tissue damage caused by it, and belongs to the technical field of prevention and treatment of acute pancreatitis. The invention proves that the long-chain inulin has a protective effect on cerulein-induced acute pancreatitis in mice, and can be added as an effective component to medicines for preventing and treating acute pancreatitis, so as to reduce the occurrence and development of clinical acute pancreatitis and the degree of inflammation. The present invention prevents the mouse model of acute pancreatitis induced by long-chain inulin, observes the pathological histomorphology of the pancreatic tissue, and detects serum pancreatic lipase, serum amylase, and inflammatory cell-releasing enzyme in the tissue. The level of MPO, the anti-inflammatory factor IL10 and the pro-inflammatory cytokines TNF-α and IL1β in tissues and serum were measured. Comparing the levels of various indicators in the long-chain inulin treatment group and the inflammation model group, the indicators in the long-chain inulin treatment group were significantly better than those in the inflammation model group.

Description

technical field [0001] The invention relates to the effect of long-chain inulin on regulating acute pancreas inflammation and related tissue damage caused by it, and belongs to the technical field of prevention and treatment of acute pancreatitis. Background technique [0002] Inulin is a mixture of natural fructans. Fructans are carbohydrates in which fructose units are connected by (2-1) chains and terminated by glucose units, and are contained in the protoplasm of cells in a colloidal form. It is mainly found in Compositae plants, for example, in the tubers of Jerusalem artichoke, the roots of Geranium (Dali Chrysanthemum), and the roots of thistles. Studies have shown that long-chain inulin in the diet can alleviate the symptoms of patients with necrotizing colitis, and a diet low in fermentable oligosaccharides, disaccharides, and polyols can effectively reduce functional gastrointestinal symptoms in patients with irritable bowel syndrome, which shows that long-chain i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/125A23L2/38A23L2/52A61K31/733A23K20/163A61P1/18
CPCA23L2/38A23L2/52A61K31/733A23V2002/00A23V2200/32A23V2250/5062A23V2250/5068
Inventor 孙嘉何月孙正华张灏陈永泉
Owner JIANGNAN UNIV
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