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Rapid propagation method for tissue culture of croomia japonica

A technology of Rhizoma Polygonatum Leaf and Glycyrrhizae, which is applied in the field of tissue culture and rapid propagation of Rhizoma Rhizoma Rhizoma Polygonatum, achieves the effects of short production cycle, stable technology and reduced damage

Active Publication Date: 2017-05-10
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Up to now, the researches on the tissue culture of Glycyrrhizae Polygonatum at home and abroad are still limited and preliminary, only using the stems of Glycyrrhizae Polygonatum to induce callus and prevent callus from browning (Li Wenping et al. , 2012; Chen Beibei et al., 2012), there is no report of callus successfully differentiated into test-tube plantlets

Method used

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  • Rapid propagation method for tissue culture of croomia japonica
  • Rapid propagation method for tissue culture of croomia japonica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Embodiment 1, a kind of tissue culture rapid propagation method of Rhizoma Polygonatum Leaf, carries out the following steps successively:

[0092] 1), drawing materials:

[0093] The adventitious buds of rhizomes of Rhizoma Polygonatum are selected as explants; specifically: the adventitious buds on rhizomes of Rhizoma Polygonatum collected from Tianmu Mountain in April are selected as explants.

[0094] 2), disinfection of adventitious buds:

[0095] Fully wash the adventitious buds on the rhizomes obtained in step 1): First, add 2 to 3 drops of detergent to each 1L of tap water for washing, then place them in a mesh bag and rinse with running water for 10 to 15 minutes; then transfer the adventitious buds to On the ultra-clean workbench, first soak it with 75% (volume %) alcohol for 20-30 seconds, rinse it with sterile water, and use 0.1% (mass %) mercury chloride (add 2-3 drops per 100mL of 0.1% mercury chloride). Tween 20) sterilized for 8-10 minutes, rinsed with...

Embodiment 2

[0115] Change the proliferation medium in step 4) to: MS+BA2mg / L+KT1mg / L+NAA0.2mg / L+2,4-D 0.5mg / L+0.8%PVP+sucrose 30g / L+agar 8g / L, pH is 5.6~5.8; Others are equal to embodiment 1.

[0116] In step 4), after 6 weeks, 5 to 7 clustered buds can be regenerated from the bud clusters in each culture container; the multiplication factor is about 5.9.

[0117] The final survival rate was 90%.

Embodiment 3

[0119] Change the proliferation medium in step 4) to MS+BA3mg / L+NAA0.2mg / L+0.8g / L PVP+sucrose 30g / L+agar 8g / L, the pH is 5.6-5.8; the rest is the same as in Example 1.

[0120] In step 4), after 6 weeks, 5 to 8 clustered buds can be regenerated from the bud clusters in each culture container. The multiplication factor is about 6.6.

[0121] The final survival rate was 90%.

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Abstract

The invention discloses a rapid propagation method for tissue culture of croomia japonica. The rapid propagation method includes the steps: 1) selecting indefinite buds of root stocks of the croomia japonica as explants; 2) disinfecting the indefinite buds; 3) startup culture of the indefinite buds: inoculating the disinfected indefinite buds without peripheral blades into a startup culture medium, growing the indefinite buds and inducing cluster buds; 4) rapidly amplifying the indefinite buds: dividing the cluster buds obtained in the step 3) into bud tussocks respectively containing a new bud, and then transferring the bud tussock onto a proliferation medium for culture; 5) elongation and rooting culture of the indefinite buds: cutting the cluster buds obtained in the step 4) into indefinite bud tussocks respectively containing 2-3 buds, transferring the indefinite bud tussocks onto a rooting / elongation culture medium for culture; 6) taking out rooted plants obtained in the step 5) to obtain seedlings capable of achieving planting out of test-tubes.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a method for tissue culture and rapid propagation of Rhizoma Polygonatum. Background technique [0002] Plants are a natural treasure house of medicines, and humans have used medicinal plants to prevent and treat diseases for thousands of years. Secondary metabolites synthesized and accumulated by plants are widely used in industries such as pharmaceuticals, food and cosmetics. my country is one of the countries with the richest medicinal plant resources in the world. With the rapid development of the Chinese medicine industry, the demand for Chinese medicine resources at home and abroad is increasing rapidly. my country also exports a large number of extracts from medicinal plants to China every year. All over the world, these are accelerating the consumption of traditional Chinese medicine resources in our country and bringing devastating harm to endangered medicinal plant ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 姜维梅邱英雄
Owner ZHEJIANG UNIV
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