Set of reagents used for identification or assistant identification of cucurbita maxima vine length characters, and cucurbita maxima vine length molecular markers

An Indian pumpkin and auxiliary identification technology, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems that the linkage between markers and short vine genes is not close enough, and it is difficult to apply

Active Publication Date: 2017-05-03
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, Wang et al. (2014) used AFLP technology to obtain molecular markers linked to the short-vine genes of Indian squash. However, due to the lack of close linkage between the markers and the short-vine genes, and the fact that AFLP markers are difficult to be directly applied to genotype screening, it is difficult Application in practical breeding

Method used

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  • Set of reagents used for identification or assistant identification of cucurbita maxima vine length characters, and cucurbita maxima vine length molecular markers
  • Set of reagents used for identification or assistant identification of cucurbita maxima vine length characters, and cucurbita maxima vine length molecular markers
  • Set of reagents used for identification or assistant identification of cucurbita maxima vine length characters, and cucurbita maxima vine length molecular markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Example 1. The set of reagents for identifying or assisting in identifying the vine growth traits of Indian pumpkin can identify the vine growth of Indian pumpkin

[0095] The set of reagents for identifying or assisting in identifying the vine growth traits of Indian pumpkin consists of primer pairs named A1 and A2 respectively; A1 is composed of single-stranded DNA named P1 and P2, and P1 is the 1-25th of SEQ ID No.1 The single-stranded DNA shown in the position, P2 is the single-stranded DNA reverse complementary to the 1429-1452 position of SEQ ID No.1; A2 is composed of the single-stranded DNA named P3 and P2, and P3 is SEQ ID No.1 Single-stranded DNA indicated at positions 307-328 of .

[0096] 1. Indian pumpkins to be identified are as follows, and 50 plants of each Indian pumpkin are selected for testing:

[0097] P1: long vine inbred line Rimu, its phenotype is long vine, and the length of vines 1-25 at 60 days after planting is 252cm.

[0098] P2: short vine...

Embodiment 2

[0127] Example 2. Application of a complete set of reagents for identifying or assisting identification of Indian pumpkin vine growth traits in cultivating short-vined Indian pumpkins

[0128] In this example, the method of backcrossing was used to select the short-vine inbred line SQ026 as the non-recurrent parent (short-vine trait donor), the long-vine inbred line Rimu as the recurrent parent, and the directional improvement of the long-vine inbred line Rimu The characteristics of vine growth, the specific operation steps are as follows:

[0129] The short vine inbred line SQ026 was crossed with the long vine inbred line Rimu to obtain the first generation of hybrid F1, and the first generation of hybrid F1 was backcrossed with the long vine inbred line Rimu to obtain the first generation of backcross BC1F1, and the Indian pumpkin genotype was identified using the present invention The complete set of reagents selects the Indian pumpkin of the RS genotype from the first-gene...

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Abstract

The invention discloses a set of reagents used for identification or assistant identification of cucurbita maxima vine length characters, and cucurbita maxima vine length molecular markers. The set of reagents are composed of a primer pair A1 and a primer pair A2; the primer pair A1 is composed of a single-stranded DNA P1 and a single-stranded DNA P2; the single-stranded DNA P1 is a single-stranded DNA capable of realizing specific binding with the upstream 93th site of a DNA represented by SEQ ID No.1; the single-stranded DNA P2 is a single-stranded DNA capable of realizing specific binding with the downstream 1341th site of the DNA represented by SEQ ID No.1; the primer pair A2 is composed of a single-stranded DNA P3 and the single-stranded DNA P2; and the single-stranded DNA P3 is a single-stranded DNA capable of realizing specific binding with the 93th to 1341th sites of the DNA represented by SEQ ID No.1. Tt is confirmed by experiments that the set of reagents can be used for identifying long vine cucurbita maxima from short vine cucurbita maxima via identifying the RR, RS, and SS genotype of cucurbita maxima.

Description

technical field [0001] The invention relates to a set of reagents for identifying or assisting identification of vine growth traits of Indian pumpkin and molecular markers for vine growth of Indian pumpkin in the field of biotechnology. Background technique [0002] Indian pumpkin is an important cucurbit vegetable widely cultivated in the world. The planting area in my country has gradually increased in recent years. Indian pumpkin can be used for both vegetables and seeds, and it is also an important source of rootstocks for grafting such as cucumbers and watermelons. The production of Indian pumpkin is of great significance to increase the income of farmers and adjust the structure of agricultural industry. [0003] Dwarfism is an important trait for improving crop yield. Indian pumpkin with short vines can increase planting density, flower and set fruit earlier, increase yield, save labor, and is suitable for centralized harvesting. It is one of the important selective ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 张国裕李海真张帆贾长才姜立纲
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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