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Microecological preparation for promoting algae growth in litopenaeus vannamei aquaculture and preparation

The technology of a micro-ecological preparation and vanabine is applied in the field of micro-ecological preparations for promoting the growth of algae and the preparation thereof, which can solve the problems of drug residues, economic losses of farmers, restricting the development of shrimp farming, and achieves inhibiting the growth of pathogenic bacteria and improving the Effects of immunity and disease resistance

Inactive Publication Date: 2017-04-26
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the high temperature season of spring and summer every year, various acute infectious diseases often break out, such as nerve necrosis virus, iridescent virus, vibrio and other pathogenic bacteria, which cause a large number of deaths of shrimp seedlings and juvenile shrimp, bringing huge economic losses to farmers. , seriously restricting the development of shrimp farming
According to statistics, the annual mortality rate of prawn seedlings is as high as 80%. At present, in order to maintain the breeding density and prevent the outbreak of diseases in the process of prawn farming, various antibiotics are blindly used in production, such as chloramphenicol, oxytetracycline, and furazolidone. etc., resulting in severe drug residues in the body of farmed animals, which has brought potential harm to the health of farmed animals and humans

Method used

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  • Microecological preparation for promoting algae growth in litopenaeus vannamei aquaculture and preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 A kind of preparation of probiotics

[0039] Cultivation of bacteria of each component in probiotics

[0040] Cultivation of Pichiaguilliermondiisp.510-6jm (Pichiaguilliermondiisp.510-6jm) CCTCC NO: M20100173: Culture the starting strain of Pichia pastoris in the order of slant, liquid seed, and liquid fermentation, slant and seed The medium is conventional medium; fermentation medium formula (W / V): yeast extract 10g / L, peptone 20g / L, glucose 20g / L, MgSO 4 ·7H 2 O 0.2g / L, KH 2 PO 4 5g / L, the fermentation culture condition is 28~30℃, aeration culture 36~48h, the number of viable bacteria measured is ≥10 9 CFU / mL.

[0041] Lactobacillus plantarum includes Lactobacillus plantarum (Lactobacillus plantarum) 1A07806 preservation number CCTCC NO: M2013293 and Lactobacillus acidophilus (Lactobacillus acidophilus) PL7 preservation number CCTCC NO: M2013294 Cultivation: the starting strains are cultivated in the order of slant, liquid seed, and liquid fermentat...

Embodiment 2

[0047] Application test 1 of embodiment 2 probiotics of the present invention in prawn culture

[0048] 1. Materials and methods

[0049] 1. Test product: the compound probiotic preparation bacterium liquid of embodiment 1, the total number of viable bacteria is more than 1 billion / mL.

[0050] 2. Test prawns: divide the prawns into 2 groups, 250 tails in each group, and set 3 replicates in each group.

[0051] 3. Test water body: The test water is taken from sand-filtered seawater in Baicheng, Xiamen.

[0052] 4. Test site: The test is carried out in a half-ton glass fiber tank. The test cylinders were placed in an open place outdoors and placed randomly under natural conditions.

[0053] 5. Test method: The test period is 45 days. The test adopts conventional prawn feed feeding, and the test group adds 1 kg of test product per ton of feed to ensure that the number of viable bacteria in each gram of feed is ≥10 6 CFU / mL, no test product was added to the control group. On...

Embodiment 3

[0063] Application test 2 of embodiment 3 probiotics of the present invention in prawn culture

[0064] 1. Test product: the compound probiotic preparation bacterium liquid of embodiment 1, the total number of viable bacteria is more than 1 billion / mL.

[0065] 2. Experimental prawns: use the whole process from throwing seedlings to growing prawns. Set up 3 experimental pools and 7 control pools.

[0066] 3. Test site: a prawn farm in Shentu Town, Zhangpu County.

[0067] 4, test method: when raising daily, get embodiment 1 compound bacterial agent pool and evenly splash. Use once every 3 days, the dosage of each mu of aquaculture water body is 1~2L, double the usage when the water quality deteriorates. The control group did not use the bacteria agent. Get the composite bacterial agent of embodiment 1 and spray evenly on the feed surface after diluting with appropriate amount of water simultaneously, use 1~2L of every 100kg feed, every day mix material and feed the prawns ...

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Abstract

The invention provides a microecological preparation for promoting algae growth in litopenaeus vannamei aquaculture and preparation and relates to aquaculture. The microecological preparation for promoting algae growth in litopenaeus vannamei aquaculture contains at least one of lactobacillus plantarum and actobacillus acidophilus and pichia guilliermondii. When the microecological preparation for promoting algae growth in litopenaeus vannamei aquaculture contains the pichia guilliermondii, the lactobacillus plantarum and the actobacillus acidophilus, the pichia guilliermondii accounts for 20-30% of the total bacteria count percentage, the lactobacillus plantarum accounts for 30-40% of the total bacteria count percentage, the actobacillus acidophilus accounts for 30-40% of the total bacteria count percentage, and the total amount is 100%. The microecological preparation for promoting algae growth in litopenaeus vannamei aquaculture can be applied to preparation of drugs for preventing and treating prawn diseases or feed additives or water purification agents or the like.

Description

technical field [0001] The invention relates to aquaculture, in particular to a microecological agent for promoting algae growth in Litopenaeus vannamei culture, a preparation method and application thereof. Background technique [0002] Litopenaeus vannamei is currently one of the three top varieties with the highest aquaculture output in the world (the other two are Penaeus monodon and Penaeus sinensis). It has the characteristics of fast growth, strong disease resistance, and significant economic benefits of aquaculture. Litopenaeus vannamei was introduced into my country in 1988 and quickly spread throughout the country. After more than 20 years of development, the culture technology of Litopenaeus vannamei has gradually matured, and the output has increased year by year. Since 2001, the aquaculture area and output of Penaeus vannamei in my country have always been the first in prawn farming, and its aquaculture volume has reached more than 70% of my country's prawn aqu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34A01K61/59A23K10/18A23K50/80A61K36/064A61K35/747A61P31/04A61P37/04C12R1/07C12R1/25C12R1/23C12R1/84
CPCA61K35/747A61K36/064C02F3/34C12N1/16C12N1/20A61K2300/00Y02A40/81
Inventor 邵宗泽单大鹏李旭林剑辉王丽萍
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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