A new type of cell cryopreservation bag meeting the requirements of aseptic operation and its application method

A technology for aseptic operation and cryopreservation bags, which is applied in the field of cryopreservation protection bags for medical biological samples and new cell cryopreservation bags, to avoid contamination risks, reduce redundant parts, and reduce workload.

Active Publication Date: 2018-01-30
YINFENG BIOLOGICAL GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(2) For long-term cryopreservation of umbilical cord blood hematopoietic stem cells, cryoprotectant must be added to protect the cells

Method used

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  • A new type of cell cryopreservation bag meeting the requirements of aseptic operation and its application method
  • A new type of cell cryopreservation bag meeting the requirements of aseptic operation and its application method
  • A new type of cell cryopreservation bag meeting the requirements of aseptic operation and its application method

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Experimental program
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Effect test

specific Embodiment 1

[0034] see figure 1 , figure 2 , image 3It can be seen that a novel cell cryopreservation bag 6 meeting the aseptic operation requirements of the present invention and its method of use are composed of a cryopreservation bag 6 and a sampling tube, one end of the sampling tube is connected to the freezing storage bag 6, and the other end of the sampling tube is provided with There is a branch pipe, and the branch pipe is provided with a nozzle of the sampling tube; the nozzle of the sampling tube includes the nozzle of the first sampling tube and the nozzle of the second sampling tube, and the nozzle of the first sampling tube is the injection port of the cryoprotectant 8 , the nozzle of the second sampling tube is double-headed, one of which is a puncture needle 5, and the other is a sampling tube nozzle 3, the nozzle of the sampling tube is provided with a sealing structure, and the sampling tube is provided with a bacteria-filtering antifouling structure, which is frozen ...

specific Embodiment 2

[0036] see figure 1 , figure 2 It can be seen from group 3 that the nozzle of the sample injection tube adopts a screw structure, and the screw is fixed on the bolt, and the screw cap is added to seal it. Catheter clip 13 can prevent contamination caused by the internal and external communication of cryopreservation bag 6, and the inner diameter of the interface between the sampling nozzle and cryopreservation agent filling port bolt 11 should be suitable for the connection after removing the needle from the syringe, generally 3.8-4.0mm; the filter can Equipped according to actual needs, the filter is a 0.22μm syringe filter 9, and the inner diameter of the corresponding interface should be suitable for connecting the fine end of the filter. Generally, the inner diameter of the interface or the inner diameter of the coarse end of the filter is 3.8-4.0mm. The 0.22 μm syringe filter 9 of the cryoprotectant injection tube is used for bacterial filtration of the cryoprotectant w...

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Abstract

The invention discloses a novel cell cryopreservation bag meeting sterile operation requirements and a use method. The novel cell cryopreservation bag comprises a cryopreservation bag and a sample feeding tube, wherein one end of the sample feeding tube is connected with the cryopreservation bag; a branch tube is arranged at the other end of the sample feeding tube; and sample feeding tube holes are formed in the branch tube. The novel cell cryopreservation bag is characterized in that the sample feeding tube holes comprise a first sample feeding tube hole and a second sample feeding tube hole; the first sample feeding tube hole is a cryopreservation protection agent injection hole; the second sample feeding tube hole comprises double heads; one head is a puncture needle; the other head is a sampling tube hole; sealing structures are arranged at the sample feeding tube holes; and a bacterium filtering anti-flouring structure is arranged at the feeding tube. As a three-end connecting conduit comprises three connecting holes which correspond to different requirements respectively, both redundant parts and workload can be reduced, and specific operation can be conveniently implemented.

Description

technical field [0001] The invention belongs to the field of new medical devices, specifically a novel cell cryopreservation bag that meets the requirements of aseptic operation and a method for using it, that is, a cryopreservation protection bag for medical biological samples (referred to as a cryopreservation bag). Background technique [0002] Stem cells are a type of cells with self-renewal and differentiation potential, and have the potential to regenerate various tissues and organs and the human body. Umbilical cord blood hematopoietic stem cells are derived from the blood remaining in the placenta and umbilical cord after the fetus is delivered, and can be used clinically to rebuild human hematopoietic and immune systems and treat various diseases. So far, the Ministry of Health has approved the establishment of seven cord blood banks in Beijing, Tianjin, Shanghai, Guangdong, Sichuan, Shandong, and Zhejiang, storing more than 800,000 units of cord blood. In order to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0263
Inventor 徐峰波生德伟崔光晶曹光鑫
Owner YINFENG BIOLOGICAL GRP
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