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Fiber carrier and preparation method and application thereof

A fiber and carrier technology, applied in the field of fiber carrier and its preparation, can solve the problems of non-hydrophilicity, small voids, and low mechanical strength, and achieve the effects of large specific surface area, improved hydrophilicity, and high mechanical strength

Active Publication Date: 2017-04-19
ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Based on this, it is necessary to provide a fiber carrier and its preparation method and application for the fiber carrier with problems such as small voids, low mechanical strength and non-hydrophilicity.

Method used

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  • Fiber carrier and preparation method and application thereof
  • Fiber carrier and preparation method and application thereof
  • Fiber carrier and preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0057] The preparation method of described fiber carrier, such as figure 1 Shown, this preparation method comprises:

[0058] S100: interweaving polymer fibers to form a fiber sheet with a sheet-like structure or a cloth-like structure.

[0059] In order to facilitate the preparation of the fiber carrier and ensure its convenient application in cell culture, it is firstly prepared into a sheet-like or cloth-like fiber sheet.

[0060] S200: Washing and drying the fiber sheet.

[0061] This step is a necessary preparatory step for the preparation of the fiber carrier. Preferably, the liquid used for cleaning is double distilled water or even ultrapure water, and the cleaning method is ultrasonic cleaning. Preferably, it is also necessary to soak the fiber sheets before cleaning to further eliminate the interference of impurities such as bacteria and microorganisms on the fiber sheets. The time is 2 to 4 hours. Preferably, the drying treatment of the washed fibers is carried ...

Embodiment 1

[0073] Preparation of fibrous carrier

[0074] Pretreatment: Select polypropylene polymer fibers with a fiber diameter of 25±2μm as raw materials, interweave them to form 30 polypropylene non-woven fiber sheets with a size of 2.5±0.2cm×0.5±0.2cm, and place 30 fiber sheets in Ultrasonic cleaning in 50ml of ultra-pure water for 10min, take out the cleaned fiber sheet and dry it with nitrogen gas. Then place it in 20ml of hydrogen peroxide solution with a volume fraction of 30% and soak for 2 hours at a temperature of 90°C, then ultrasonically clean the soaked fiber sheet with ultrapure water for 20 minutes, and dry it at 90°C for 3 hours.

[0075] Aminosilane treatment: add the pretreated fiber sheet to a toluene solution containing 5% 3-aminopropyltriethoxysilane, take it out after the reaction, put it in the toluene solution, ultrasonically clean it for 10 minutes, and then dry it with nitrogen.

[0076] Glutaraldehyde coupling: Soak the fiber sheet treated with aminosilane i...

Embodiment 2

[0080] Preparation of fibrous carrier

[0081] Pretreatment: Select polypropylene polymer fibers with a fiber diameter of 20±2μm as raw materials, interweave them to form 30 polypropylene non-woven fiber sheets with a size of 2.5±0.2cm×0.5±0.2cm, place the fiber sheets in a 50ml supernatant Ultrasonic cleaning in pure water for 20 min, take out the cleaned fiber sheet and dry it with nitrogen gas. Then place it in 20ml of hydrogen peroxide solution with a volume fraction of 30% and soak for 3 hours at a temperature of 90°C, then ultrasonically clean the soaked fiber sheet with ultrapure water for 30 minutes, and dry it at 90°C for 4 hours.

[0082] Aminosilane treatment: Add the pretreated fiber sheet into a toluene solution containing 5% 3-aminopropyltrimethoxysilane, take it out after the reaction, place it in the toluene solution, ultrasonically clean it for 20 minutes, and then dry it with nitrogen.

[0083] Glutaraldehyde coupling: Soak the fiber sheet treated with amino...

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Abstract

The invention provides a fiber carrier and also provides a preparation and application of the fiber carrier. The fiber carrier comprises polymer fiber and a collagen coating which covers the surface of the polymer fiber. The diameter of the polymer fiber is 15-30 microns, and the thickness of the collagen coating is 1.5-300 nm. The fiber carrier has large gap, high mechanical strength and good hydrophily. The fiber carrier is a fiber carrier with excellent properties and for cell culture. The preparation method of the fiber carrier has simple steps, has no high requirements on instruments and equipment, and is suitable for various scales of production.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a fiber carrier and its preparation method and application. Background technique [0002] Microcarrier culture is currently mainly used in the large-scale culture technology of animal cells. Because cells have the characteristics of adherent growth, and microcarriers can increase the area of ​​cell attachment and growth, they are widely used in the production of various cells and cell products, including biological products such as enzymes, growth factors, vaccines and antibodies. [0003] At present, most of the microcarriers used in domestic and foreign markets are in the form of microspheres. According to the cross-linked macromolecules of dextran with different groups, microcarriers are mainly divided into two categories: one is a positively charged compound diethylaminoethyl (DEAE) as a ligand, It is used for general animal cell culture, such as Cytodex 1 developed by Pharmac...

Claims

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Application Information

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IPC IPC(8): D06M13/513D06M13/123D06M15/15D06M13/342D06M10/00C12M3/00C12N5/07D06M101/20D06M101/32D06M101/34
CPCC12M25/10C12N5/0075C12N2513/00C12N2531/00C12N2533/30C12N2533/54C12N2533/56D06M10/008D06M13/123D06M13/342D06M13/513D06M15/15D06M16/00D06M2101/20D06M2101/32D06M2101/34
Inventor 高毅彭公泽张贵锋康跻耀李阳
Owner ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV
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