Method for increasing reducing sugar content of straws
A technology for sugar content and straw is applied in the field of increasing the reducing sugar content of straw, which can solve the problems of restricting the effective progress of biodegradation reaction, expensive cellulase, restriction of cellulase action, etc., and achieves increased yield content and loose structure. , the effect of reducing toughness
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Embodiment 1
[0043]A method for increasing the reducing sugar content of stalks, comprising the following steps in sequence:
[0044] 1) Pulverization: pulverize the mixture of straws of wheat, rice, corn, millet, sugar cane and cotton into a powder with a particle size of 200 mesh.
[0045] 2) Carrying out electron beam irradiation to the straw, the electron beam irradiation dose is 950kGy;
[0046] 3) Ultraviolet treatment: put the stalks irradiated by electron beams under ultraviolet light for 30 minutes;
[0047] 4) acidify the straw in a hydrochloric acid solution with a volume concentration of 30% for 12 hours, and then cook for 2 hours at an initial temperature of 50°C and a heating rate of 0.5°C / min;
[0048] 5) First adjust the pH of the cooked straw liquid to 4.9, and the temperature is below 60 degrees Celsius, then add cellulase to it according to the enzyme to straw mass ratio of 6:1000, enzymolysis for 5 hours, the filter paper enzyme activity of the cellulase It is 1400FPU...
Embodiment 2
[0053] A method for increasing the reducing sugar content of stalks, comprising the following steps in sequence:
[0054] 1) Pulverization: pulverize the mixture of straws of wheat, rice, corn, millet, sugar cane and cotton into a powder with a particle size of 300 mesh.
[0055] 2) Carrying out electron beam irradiation to the straw, the electron beam irradiation dose is 1250kGy;
[0056] 3) Ultraviolet treatment: put the stalks irradiated by electron beams under ultraviolet lamps and irradiate them for 40 minutes;
[0057] 4) Acidify the straw in a hydrochloric acid solution with a volume concentration of 30% to 35% for 12 to 24 hours, and then cook for 6 hours at an initial temperature of 50°C and a heating rate of 0.5°C / min;
[0058] 5) First adjust the pH of the cooked straw liquid to 4.9, and the temperature is below 60 degrees Celsius, then add cellulase to it according to the mass ratio of enzyme to straw of 9:1000, enzymolysis for 24 hours, the filter paper enzyme ac...
Embodiment 3
[0063] A method for increasing the reducing sugar content of stalks, comprising the following steps in sequence:
[0064] 1) Pulverization: pulverize the mixture of straws of wheat, rice, corn, millet, sugar cane and cotton into a powder with a particle size of 250 mesh.
[0065] 2) Carrying out electron beam irradiation to the straw, the electron beam irradiation dose is 1350kGy;
[0066] 3) Ultraviolet treatment: put the stalks irradiated by electron beams under ultraviolet lamps and irradiate for 35 minutes;
[0067] 4) Acidifying the straw in a hydrochloric acid solution with a volume concentration of 30% to 35% for 18 hours, and then cooking for 4 hours at an initial temperature of 50°C and a heating rate of 0.5°C / min;
[0068] 5) First adjust the pH of the cooked straw liquid to 4.9, and the temperature is below 60 degrees Celsius, then add cellulase to it according to the mass ratio of enzyme to straw of 7.5:1000, enzymolysis for 14 hours, the filter paper enzyme activ...
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