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Method for increasing reducing sugar content of straws

A technology for sugar content and straw is applied in the field of increasing the reducing sugar content of straw, which can solve the problems of restricting the effective progress of biodegradation reaction, expensive cellulase, restriction of cellulase action, etc., and achieves increased yield content and loose structure. , the effect of reducing toughness

Inactive Publication Date: 2017-04-19
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the complex structure of straw and the crystalline region of cellulose limit the action of cellulase, and it is difficult for cellulase to enter it, which restricts the effective progress of biodegradation reactions.
At the same time, due to the relatively expensive price of cellulase

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043]A method for increasing the reducing sugar content of stalks, comprising the following steps in sequence:

[0044] 1) Pulverization: pulverize the mixture of straws of wheat, rice, corn, millet, sugar cane and cotton into a powder with a particle size of 200 mesh.

[0045] 2) Carrying out electron beam irradiation to the straw, the electron beam irradiation dose is 950kGy;

[0046] 3) Ultraviolet treatment: put the stalks irradiated by electron beams under ultraviolet light for 30 minutes;

[0047] 4) acidify the straw in a hydrochloric acid solution with a volume concentration of 30% for 12 hours, and then cook for 2 hours at an initial temperature of 50°C and a heating rate of 0.5°C / min;

[0048] 5) First adjust the pH of the cooked straw liquid to 4.9, and the temperature is below 60 degrees Celsius, then add cellulase to it according to the enzyme to straw mass ratio of 6:1000, enzymolysis for 5 hours, the filter paper enzyme activity of the cellulase It is 1400FPU...

Embodiment 2

[0053] A method for increasing the reducing sugar content of stalks, comprising the following steps in sequence:

[0054] 1) Pulverization: pulverize the mixture of straws of wheat, rice, corn, millet, sugar cane and cotton into a powder with a particle size of 300 mesh.

[0055] 2) Carrying out electron beam irradiation to the straw, the electron beam irradiation dose is 1250kGy;

[0056] 3) Ultraviolet treatment: put the stalks irradiated by electron beams under ultraviolet lamps and irradiate them for 40 minutes;

[0057] 4) Acidify the straw in a hydrochloric acid solution with a volume concentration of 30% to 35% for 12 to 24 hours, and then cook for 6 hours at an initial temperature of 50°C and a heating rate of 0.5°C / min;

[0058] 5) First adjust the pH of the cooked straw liquid to 4.9, and the temperature is below 60 degrees Celsius, then add cellulase to it according to the mass ratio of enzyme to straw of 9:1000, enzymolysis for 24 hours, the filter paper enzyme ac...

Embodiment 3

[0063] A method for increasing the reducing sugar content of stalks, comprising the following steps in sequence:

[0064] 1) Pulverization: pulverize the mixture of straws of wheat, rice, corn, millet, sugar cane and cotton into a powder with a particle size of 250 mesh.

[0065] 2) Carrying out electron beam irradiation to the straw, the electron beam irradiation dose is 1350kGy;

[0066] 3) Ultraviolet treatment: put the stalks irradiated by electron beams under ultraviolet lamps and irradiate for 35 minutes;

[0067] 4) Acidifying the straw in a hydrochloric acid solution with a volume concentration of 30% to 35% for 18 hours, and then cooking for 4 hours at an initial temperature of 50°C and a heating rate of 0.5°C / min;

[0068] 5) First adjust the pH of the cooked straw liquid to 4.9, and the temperature is below 60 degrees Celsius, then add cellulase to it according to the mass ratio of enzyme to straw of 7.5:1000, enzymolysis for 14 hours, the filter paper enzyme activ...

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Abstract

The invention discloses a method for increasing reducing sugar content of straws. The method comprises the following steps carried out in sequence: 1, performing electron beam irradiation on the straws; 2, putting the straws into a hydrochloric acid solution with the volume concentration of 30-35% to perform acidification for 12-24 hours, and performing stewing for 2-6 hours at a starting temperature of 50 DEG C and a heating rate of 0.5 DEG C / min; and 3, adjusting the pH value and temperature of a stewed straw solution to 4.9 and 60 DEG C or below respectively, adding cellulase to the straw solution according to a mass ratio of the cellulase to the straws of 6-9 : 1,000, and performing enzymolysis for 5-24 hours, wherein the filter paper enzyme activity of the cellulase is 1,400-1,500 FPU / g. According to the method, the tissue structures of the straws are greatly changed and destroyed; the utilization amount of the cellulase is reduced; the production cost is reduced; the reducing sugar content in the straws is greatly increased after processing; and the method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of bioenergy, and in particular relates to a method for increasing the reducing sugar content of straw. Background technique [0002] At present, with the rapid development of industrial economy, traditional energy consumption is gradually increasing. According to the current rate of extraction and consumption, oil, natural gas and coal reserves can only supply human beings for several decades. On the other hand, with the extensive use of traditional energy sources, the problem of environmental pollution has become more and more serious, and human beings are facing the dual pressure of resource shortage and environmental pollution. In order to effectively deal with this global problem, more and more countries are focusing on the development and utilization of renewable energy (A.J.Ragauskas, 2006). [0003] At present, biomass energy has increasingly become a development hotspot due to its advantages of la...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
CPCC12P19/14C12P2201/00
Inventor 哈益明靳婧王锋唐洪涛李庆鹏郭芹
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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