Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Small-molecule fluorescent probe for rapidly identifying superoxide radicals, preparation method and application thereof

A fluorescent probe and superoxide technology, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, analytical materials, etc., can solve the problems of long detection time and inability to realize cell imaging, and achieve simple post-processing process and resistance to other molecules Strong interference ability and good selectivity

Inactive Publication Date: 2017-04-19
UNIV OF JINAN
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are shortcomings such as long detection time and inability to realize cell imaging.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Small-molecule fluorescent probe for rapidly identifying superoxide radicals, preparation method and application thereof
  • Small-molecule fluorescent probe for rapidly identifying superoxide radicals, preparation method and application thereof
  • Small-molecule fluorescent probe for rapidly identifying superoxide radicals, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Synthesis of probe compound NS-O:

[0022]

[0023] Compound 1 (0.5mmol, 86.0mg) and compound 2 (1.1equiv, 68.8mg) were added to the reaction flask under a nitrogen atmosphere, and then ethanol (5.0mL) was added to the above reactor at one time, and the After heating to reflux for 5 hours, the reaction was detected by spotting until the raw materials disappeared, and the solvent was spin-dried under reduced pressure to obtain a crude product, which was separated with a silica gel column to obtain the probe compound NS-O, the silica gel particle size was 200-300 mesh, and the yield was 63%. 1 H-NMR (400MHz, DMSO-d 6 )δ10.12(s,1H),8.56(s,1H),8.17-8.01(m,4H),7.86(d,J=8.8Hz,1H),7.58-7.45(m,2H),7.22-7.18 (m,2H). The probe's 1 H NMR spectrum see figure 1 .

Embodiment 2

[0025] Probe NS-O with O 2 - The addition of its visible light absorption changes

[0026] The probe NS-O prepared in Example 1 was dissolved in N,N-dimethylformamide (DMF) to prepare a 1 mmol / L stock solution. Take 30 μL from the stock solution and add it to a 5mL centrifuge tube, measure its absorption spectrum, and then add O 2 - (10equiv) standard solution, diluted to 3mL (10μM) with DMSO / PBS (1:1, v / v), and measure its absorption spectrum. Spectrum see figure 2 shown. Depend on figure 2 It can be seen that after adding probe NS-O, directly add O 2 - , the change in absorption can be detected instantaneously, realizing the O 2 - detection.

Embodiment 3

[0028] Probe compound NS-O fluorescent probe with O 2 - The change of fluorescence spectrum with the increase of equivalent

[0029] Take out 30 μ L and join among the centrifuge tube of 5mL from the stock solution of embodiment 2, add different equivalent (0-70equiv) O 2 - The standard solution was diluted to 3mL with PBS buffer solution (0.1mol / L, pH=7.4) and DMSO at a volume ratio of 1:1, and its fluorescence properties were measured with 400nm as excitation light. Fluorescence spectra such as image 3 shown. Depend on image 3 It can be seen that with O 2 - Fluorescence gradually increased with the addition of equivalents.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a small-molecule fluorescent probe for rapidly identifying superoxide radicals, a preparation method and the application thereof, and belongs to the field of organic small-molecule fluorescent probes. The structural formula of the probe molecule is shown in formula (I). The designed small-molecule fluorescent probe for rapidly identifying superoxide radicals is simple to prepare, and mature in synthesis route. By means of the small-molecule fluorescent probe, O<2-> in cells can be rapidly and accurately detected, and fluorescence imaging studies can be performed. Therefore, the fluorescent probe can be used as a simple, rapid and sensitive O<2->-specific detection reagent and has a wide application prospect in the field of biological molecule detection.

Description

technical field [0001] The invention relates to a small-molecule fluorescent probe for quickly identifying superoxide radicals, a preparation method and application thereof, and belongs to the field of organic small-molecule fluorescent probes. Background technique [0002] ROS species are produced by oxidative energy metabolism and are generally considered to be ROS radicals or other species that can be converted into ROS radicals. They play a very important role in cellular function and enable the immune system to defend against the attack of foreign microorganisms, such as: bacteria, viruses and parasites. Active oxygen species mainly include: superoxide (O 2 - ), hydrogen peroxide (H 2 o 2 ), hydroxyl radical ( OH ) and nitrate (NO 3 - )Wait. Although some reactive oxygen species are necessary in living organisms, the imbalance of reactive oxygen species can also cause a series of diseases, such as: central nervous system disorders, cancer, arthritis, arterioscler...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D277/66C09K11/06G01N21/64G01N21/29
CPCC07D277/66C09K11/06C09K2211/1037G01N21/293G01N21/6428G01N21/6486
Inventor 林伟英王建勇刘展榕
Owner UNIV OF JINAN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com