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Targeting fluorescent-magnetic nano-material as well as preparation method and application thereof

A magnetic nano-targeted technology, applied in the field of nano-biomedical materials, can solve the problems of weakening overall performance, application of bound fluorescent magnetic composite nanoparticles, toxicity, etc., and achieves good magnetic separation, simple synthesis method, and biocompatibility. Good results

Inactive Publication Date: 2017-04-19
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the nano-particles doped by this method are located on the surface of the microspheres, making it easy to detach from the microspheres, which will cause the weakening of the overall performance and potential toxicity, thus seriously restricting the fluorescent magnetic composite prepared by this method. Applications of Nanoparticles

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Zn 0.4 Fe 2.6 o 4 Preparation of NPs

[0028] FeSO 4 ·(NH 4 ) 2 SO 4 ·6H 2 O and ZnSO 4 Dissolve in 20ml of water so that the precursor reaches 1.73×10 -3 mol Fe 2+ and 2.67×10 -4 mol Zn 2+ the goal of. Next, mix 10ml of oleic acid, 10ml of absolute ethanol and 1g of NaOH, and stir magnetically at room temperature until a uniform solution is obtained. Furthermore, the precursor Fe 2+ and Zn 2+ The mixed solution was poured into the homogeneous solution, and after stirring for a few minutes, the mixed solution turned dark brown. Finally, the solution was transferred into a 50ml reaction kettle, sealed, and heated at 230 degrees for 15 hours. After the reaction, cool to room temperature. The product is deposited at the bottom of the kettle, and the nanoparticles are dispersed in cyclohexane and taken out. Add ethanol to the n-hexane dispersed with nanoparticles to precipitate the nanoparticles, and finally wash the nanoparticles repeatedly with et...

Embodiment 2

[0035] Steps 1 and 2 are the same as in Example 1,

[0036] 3. RGERPPR-PEG-DSPE wrapped Zn 0.4 Fe 2.6 o 4 Preparation of NPs and CQDs

[0037] Dissolve RGERPPR-PEG-DSPE in 5ml of chloroform and transfer to a 50ml eggplant bottle, then add 5ml of Zn dispersed in n-hexane 0.4 Fe 2.6 o 4 NPs and CQDs (Zn 0.4 Fe 2.6 o 4 The ratio of the amount of NPs to CQDs is 2:1), so that RGERPPR-PEG-DSPE and Zn 0.4 Fe 2.6 o 4 The ratio of the mass sum of NPs and CQDs is 2:1, and the ultrasonic mixing is uniform. Finally, 5ml of deionized water was slowly added to the mixture. At a temperature of 70° C., rotary evaporation was performed for 15 minutes until the chloroform and n-hexane were completely evaporated. Excess empty liposome micelles were removed by magnetic separation, and larger aggregates were removed by centrifugation at 3000g, and the product was redispersed in deionized water.

[0038] Characterized RGERPPR-PEG-DSPE wrapped Zn 0.4 Fe 2.6 o 4 The water and ki...

Embodiment 3

[0040] Steps 1 and 2 are the same as in Example 1,

[0041] 3. RGERPPR-PEG-DSPE wrapped Zn 0.4 Fe 2.6 o 4 Preparation of NPs and CQDs

[0042] Dissolve RGERPPR-PEG-DSPE in 5ml of chloroform and transfer to a 50ml eggplant bottle, then add 5ml of Zn dispersed in n-hexane 0.4 Fe 2.6 o 4 NPs and CQDs (Zn 0.4 Fe 2.6 o 4 The ratio of the amount of NPs to CQDs is 1:2), so that RGERPPR-PEG-DSPE and Zn 0.4 Fe 2.6 o 4 The ratio of the mass sum of NPs and CQDs is 2:1, and the ultrasonic mixing is uniform. Finally, 5ml of deionized water was slowly added to the mixture. At a temperature of 70° C., rotary evaporation was performed for 15 minutes until the chloroform and n-hexane were completely evaporated. Excess empty liposome micelles were removed by magnetic separation, and larger aggregates were removed by centrifugation at 3000g, and the product was redispersed in deionized water.

[0043] Characterized RGERPPR-PEG-DSPE wrapped Zn 0.4 Fe 2.6 o 4 The water and ki...

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Abstract

The invention relates to a targeting fluorescent-magnetic nano-material as well as a preparation method and an application thereof. The preparation method comprises steps of preparation of Zn0.4Fe2.6O4 NPs, preparation of a carbon quantum dot and preparation of RGERPPR PEG DSPE covered Zn0.4Fe2.6O4 NPs and CQDs. The material has the characteristics of being good in malignant tumor targeting property, stable in performance, good in biocompatibility and being relatively strong in both fluorescent and magnetic saturation values. The novel targeting fluorescent-magnetic multifunctional nano-material prepared by the invention simultaneously reserves advantages of a fluorescent material and a magnetic material, and the nano-material has relatively good performances of magnetic separation, magnetic resonance imaging, fluorescent imaging and the like.

Description

technical field [0001] The present invention relates to a targeted fluorescent magnetic nanomaterial and its preparation method and application, in particular to a DSPE-PEG2000 functionalized with a tumor penetrating peptide (RGERPPR), coated with a magnetic nanomaterial (Zn 0.4 Fe 2.6 o 4 NPs) and fluorescent nanomaterials (carbon quantum dots, CQDs). The invention belongs to the field of nano biomedical materials. Background technique [0002] In recent years, multifunctional nanomaterials have attracted extensive attention in biomedical fields (such as imaging, gene regulation, drug delivery, anticancer therapy, etc.) (ACS Nano2011, 5, 3043−3051). Composite nanoparticles composed of particles with two or more functionalities represent an important class of nanoscale systems (Adv. Mater. 2009, 21, 3045−3052). At present, fluorescent nanomaterials and magnetic nanomaterials have become an important part of molecular and cell level research in the fields of chemistry, m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/14A61K49/00
CPCA61K49/0019A61K49/0056A61K49/0067A61K49/14
Inventor 何丹农祝闪闪王萍金彩虹
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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