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Polypeptide micro-array chip for identifying sources of panax ginseng

A peptide microarray and chip technology, applied in the field of biological detection, can solve problems such as cumbersome process, difficult screening and identification of ginseng sources, lack of specificity, etc.

Active Publication Date: 2017-03-29
BEIJING PEPMAGIC BIOTECH CORPORATION LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this identification method is cumbersome and lacks specificity, making it difficult to quickly screen and identify ginseng sources on a large scale

Method used

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  • Polypeptide micro-array chip for identifying sources of panax ginseng
  • Polypeptide micro-array chip for identifying sources of panax ginseng
  • Polypeptide micro-array chip for identifying sources of panax ginseng

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] 1.1 Preparation of various amino acid activation solutions

[0059] 1.1.1 Prepare amino acid activator: use DMF as solvent to prepare 0.33M DIC solution;

[0060] 1.1.2 Prepare various amino acid stock solutions: use DMF as solvent to prepare 0.33M HOBt solution; then use 0.33M HOBt solution as solvent to prepare 20 different α-amino acids protected by Fmoc, and the concentration of amino acids 0.33M;

[0061] 1.1.3 Prepare various amino acid activation solutions: mix the amino acid activator prepared in step 1.1.1 with the amino acid stock solution prepared in step 1.1.2 at a ratio of 1:1 to obtain various amino acid activation solutions of 0.165M, Let it stand at room temperature for more than 15 minutes before use.

[0062] 1.2 Substrate pretreatment

[0063] Firstly, the cellulose membrane with hydroxyl groups on the surface was stained in 0.1% bromophenol blue ethanol solution for 30s; then it was eluted twice with absolute ethanol for 2min each time; finally, i...

Embodiment 2

[0080] According to the same method as in Example 1, a polypeptide microarray chip comprising 4 regions was prepared, and these 4 regions respectively contained polypeptides having the following sequences: DLL L A N P F V, M G H V NF GA, M M R Y Y V A G and NF L R I H M T.

Embodiment 3

[0082] According to the same method as in Example 1, a polypeptide microarray chip comprising 4 regions was prepared, and these 4 regions respectively contained polypeptides having the following sequences: F G E H E R F T, L G H E F SL N, T E V W S S R C and L M N F E R E Y.

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Abstract

The invention provides a polypeptide micro-array chip for identifying sources of panax ginseng on the basis of discovering a polypeptide sequence capable of being in a specific reaction with panax ginseng total proteins extracted from the panax ginseng of separate sources and utilizing a polypeptide micro-array technology.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a biological chip for identifying the source of ginseng. Background technique [0002] Ginseng is widely used to delay aging, prevent and treat depression, especially various learning and memory impairment diseases caused by aging and stress. Drugs and health care products derived from ginseng are the varieties with the largest market share of traditional Chinese medicine products in my country. In order to meet the growing market demand, different ginseng varieties have appeared. At present, the ginseng in our country is mainly under-forest ginseng and garden ginseng, and the production areas are mainly concentrated in the northeastern region of the cold temperate zone: Heilongjiang Province and Jilin Province. Under-forest ginseng is artificially planted in a mixed coniferous and broad-leaved forest composed of trees, shrubs, and weeds. It grows purely naturally without art...

Claims

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Application Information

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IPC IPC(8): C40B40/10C40B50/06G01N33/68C07K7/06
CPCY02P20/55
Inventor 赵树民鲍勇刚石松传王利峡邱国松
Owner BEIJING PEPMAGIC BIOTECH CORPORATION LTD
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