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A Polypeptide Microarray Chip Used to Identify the Origin of Ginseng

A peptide microarray and chip technology, applied in the field of biological detection, can solve problems such as difficult screening and identification of ginseng origin, cumbersome process, and lack of specificity

Active Publication Date: 2019-06-28
BEIJING PEPMAGIC BIOTECH CORPORATION LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this identification method is cumbersome and lacks specificity, so it is difficult to quickly identify the origin of ginseng on a large scale.

Method used

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  • A Polypeptide Microarray Chip Used to Identify the Origin of Ginseng
  • A Polypeptide Microarray Chip Used to Identify the Origin of Ginseng
  • A Polypeptide Microarray Chip Used to Identify the Origin of Ginseng

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] 1.1 Preparation of various amino acid activation solutions

[0065] 1.1.1 Prepare amino acid activator: use DMF as solvent to prepare 0.33M DIC solution;

[0066] 1.1.2 Prepare various amino acid stock solutions: use DMF as solvent to prepare 0.33M HOBt solution; then use 0.33M HOBt solution as solvent to prepare 20 different α-amino acids protected by Fmoc, and the concentration of amino acids 0.33M;

[0067] 1.1.3 Prepare various amino acid activation solutions: mix the amino acid activator prepared in step 1.1.1 with the amino acid stock solution prepared in step 1.1.2 at a ratio of 1:1 to obtain various amino acid activation solutions of 0.165M, Let it stand at room temperature for more than 15 minutes before use.

[0068] 1.2 Substrate pretreatment

[0069] Firstly, the cellulose membrane with hydroxyl groups on the surface was stained in 0.1% bromophenol blue ethanol solution for 30s; then it was eluted twice with absolute ethanol for 2min each time; finally, i...

Embodiment 2

[0086] According to the same method as in Example 1, a polypeptide microarray chip comprising 4 regions was prepared, and the 4 regions respectively contained polypeptides having the following sequences: TYPCVRLN, HWYKRNIL, PLYLRAFP, and PGADDFPIF.

Embodiment 3

[0088] According to the same method as in Example 1, a polypeptide microarray chip comprising 4 regions was prepared, and these 4 regions respectively contained polypeptides having the following sequences: F P T Y D M NK, H Q S N Y I A A, W P R D N D S I and ND RP L D I L.

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Abstract

The present invention provides a polypeptide microarray chip for identifying the production place of ginseng by using a polypeptide microarray technology base on a polypeptide sequence capable of specifically reacting with the ginseng total proteins extracted from ginsengs from different production places.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a biological chip for identifying the origin of ginseng. Background technique [0002] Ginseng is widely used to delay aging, prevent and treat depression, especially various learning and memory impairment diseases caused by aging and stress. Drugs and health care products derived from ginseng are the varieties with the largest market share of traditional Chinese medicine products in my country. At present, ginseng is mainly cultivated in my country, and its production areas are mainly concentrated in the northeastern region of the cold temperate zone: Heilongjiang Province and Jilin Province. The ginseng production of these two provinces accounts for more than 80% of the whole country. Due to the influence of the growth environment, ginseng from different origins has different types and contents of main medicinal components such as ginsenosides and ginseng polysaccharides, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 赵树民鲍勇刚石松传王利峡邱国松
Owner BEIJING PEPMAGIC BIOTECH CORPORATION LTD
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