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Applications of compatibility of lycopene and sesamol in livestock semen freezing storage agents

A cryopreservative and lycopene technology, which can be used in applications, preservation of human or animal bodies, animal husbandry, etc., can solve problems such as low sperm motility, and achieve the effect of maintaining fertilization vitality, reliable effect and easy promotion.

Active Publication Date: 2017-03-29
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the present invention provides a cryopreservative for livestock semen and its application in cryopreservatives for semen of pigs, sheep and cattle, which solves the problem of low viability of livestock sperm in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation method of pig semen cryopreservation agent of the present invention, comprises the steps:

[0027] Step 1, prepare the frozen base solution: accurately measure 1.1g of glucose, 1.48g of citric acid, 2.42g of Tris, 0.06g of penicillin sodium, and 0.1g of streptomycin sulfate, dissolve them in 100ml double distilled water, adjust the pH to 6.21, and adjust the osmotic pressure 286mosm / L, prepared as frozen base fluid;

[0028] Step 2: Accurately measure 80ml of frozen base solution, add 20ml of fresh egg yolk, 0.15μmol-0.35μmol lycopene and 15mg-30mg sesamol, and mix well to obtain a cryopreservative for livestock semen of the present invention.

[0029] Take 94ml of the above livestock semen cryopreservative and add 6ml glycerin, adjust the pH value of the mixed solution to 6.0-7.5, and the osmotic pressure to 286mosm / L, filter and sterilize, cool to room temperature, and then another frozen livestock semen of the present invention can be obtained. The p...

Embodiment 2

[0056] The preparation method of goat semen cryopreservation agent of the present invention, comprises the steps:

[0057] Step 1, preparing frozen base liquid, the method is the same as in Example 1;

[0058] Step 2: Accurately measure 85ml of frozen base solution, add 15ml of fresh egg yolk, 0.20μmol-0.40μmol lycopene and 10mg-25mg sesamol, and mix well to prepare a cryopreservative for livestock semen of the present invention.

[0059] Get 90ml of the above-mentioned livestock semen cryopreservation agent and add 10ml glycerin to be mixed with 100ml of mixed solution, adjust the pH value of the mixed solution to 6.0~7.5, osmotic pressure 286mosm / L, sterilize by filtration, cool to room temperature, and obtain the other product of the present invention. A livestock semen cryopreservation agent is used to preserve sheep semen, and put it in a refrigerator at 4°C for the following experiments.

[0060] (1) Semen collection

[0061] Semen was collected by the pseudo-vaginal m...

Embodiment 3

[0078] The preparation method of bovine semen cryopreservation agent of the present invention, comprises the steps:

[0079] Step 1, preparing frozen base liquid, the method is the same as in Example 1;

[0080] Step 2: Accurately measure 74ml of frozen base solution, add 20ml of fresh egg yolk, 0.2μmol-0.4μmol lycopene, and 15mg-30mg sesamol to obtain a livestock semen cryopreservative of the present invention.

[0081] Add 6ml of glycerin to the above livestock semen cryopreservation agent, mix well, adjust the pH value to 6.0-7.5, osmotic pressure 286mosm / L, filter and sterilize, cool to room temperature, and obtain another livestock semen cryopreservation agent of the present invention It is used to preserve bovine semen and put it in a refrigerator at 4°C for the following experiments.

[0082] (1) Semen collection

[0083] Semen was collected by the pseudo-vaginal method, and routine quality inspection was carried out at 37°C with a microscope. Select semen with no pe...

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PUM

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Abstract

The present invention discloses applications of the compatibility of lycopene and sesamol in livestock semen freezing storage agents, and provides a livestock semen freezing storage agent, which can significantly improve the survival rate of livestock sperms, and comprises a freezing base liquid, egg yolk, lycopene and sesamol. The present invention further provides another livestock semen freezing storage agent, which comprises a freezing base liquid, egg yolk, lycopene, sesamol and glycerol. According to the present invention, the compatibility of lycopene and sesamol is firstly used as the anti-freezing agent for the freezing storage of the semen of pigs, sheep and cows, and the obtained livestock semen freezing storage agent can be used for the artificial insemination of the test tube freezing semen of pigs, sheep and cows, and the long-term storage and the after-long-distance-transportation semen deposition of the semen after the super-temperature freezing, and can significantly improve the survival rate, the acrosome integrity and the plasma membrane integrity of the frozen-thawed perms of pigs, sheep and cows,.

Description

technical field [0001] The invention belongs to the technical field of ultra-low temperature cryopreservation of mammalian semen, and in particular relates to the application of the combination of lycopene and sesamol as an antifreeze agent in the cryopreservation of livestock semen Background technique [0002] The development of semen cryopreservation technology solves the problem that semen cannot be preserved for a long time, and separates semen collection and fertilization in time and space, which greatly prolongs the storage time of sperm in vitro, improves the utilization rate of excellent breeding stock, and facilitates precious inheritance material preservation. [0003] Sperm are sensitive to temperature changes. In the process of freezing and cooling, sperm are susceptible to freezing damage, mainly including physical damage, chemical damage and oxidative damage. However, compared with fresh semen, the motility rate, acrosome integrity rate, mitochondrial activit...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 李青旺王捷杨丽江中良
Owner NORTHWEST A & F UNIV
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