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Tissue culturing method of physalis angulata

A technology of tissue culture and bitter beetroot, which is applied in the field of plant tissue culture, can solve the problems of slow reproduction speed and low germination rate of bitter beetroot seeds, and achieve the effects of fast reproduction speed, uniform reproductive offspring, and large reproduction coefficient

Active Publication Date: 2017-03-29
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a tissue culture method of bitter rosin, which solves the problem of slow propagation speed of bitter rosin due to the low germination rate of seeds

Method used

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  • Tissue culturing method of physalis angulata
  • Tissue culturing method of physalis angulata
  • Tissue culturing method of physalis angulata

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The seeds of A. plump were used as experimental materials. The materials were wild provenances from Pujiang, Zhejiang collected by the Zhejiang Provincial Key Laboratory of Germplasm Improvement and Quality Control Technology of Medicinal Plants. They were planted in the experimental field of Xiasha Campus of Hangzhou Normal University, and the mature fruits were taken and peeled off. , wash out the seeds, dry them on silica gel and store them in the refrigerator at 4°C in the laboratory for later use, and cultivate them according to the following steps, such as figure 1 Specific steps are as follows:

[0031] (1) Aseptic germination of seeds: Select seeds with full granules for experiment. The seeds were shaken and soaked in 95% ethanol for 5 min. Then add sodium hypochlorite in 20% alcohol volume and Tween 20 in 0.1% alcohol volume, and continue soaking for 20 minutes. After pouring off the liquid, use sterile ddH 2 O Wash the seeds 3-5 times. Sow the seeds evenly...

Embodiment 2

[0043]The seeds of A. plump were used as test materials. The materials were the wild provenances of Pujiang, Zhejiang collected by the Key Laboratory of Germplasm Improvement and Quality Control Technology of Zhejiang Province. They were planted in the experimental field of Xiasha Campus of Hangzhou Normal University, and the mature fruits were taken and peeled off. , wash out the seeds, dry them on silica gel and store them in the refrigerator at 4°C for later use, and cultivate them according to the following steps, the specific steps are as follows:

[0044] (1) Aseptic germination of seeds: Select seeds with full granules for experiment. The seeds were shaken and soaked in 95% ethanol for 5 min. Then add sodium hypochlorite in 20% alcohol volume and Tween 20 in 0.1% alcohol volume, and continue soaking for 20 minutes. After pouring off the liquid, use sterile ddH 2 O Wash the seeds 3-5 times. Sow the seeds evenly on the glass petri dish containing 1 / 2 MS medium, seal it...

Embodiment 3

[0050] The seeds of A. plump were used as test materials. The materials were the wild provenances of Pujiang, Zhejiang collected by the Key Laboratory of Germplasm Improvement and Quality Control Technology of Zhejiang Province. They were planted in the experimental field of Xiasha Campus of Hangzhou Normal University, and the mature fruits were taken and peeled off. , wash out the seeds, dry them on silica gel and store them in the refrigerator at 4°C for later use, and cultivate them according to the following steps, the specific steps are as follows:

[0051] (1) Aseptic germination of seeds: Select seeds with full granules for experiment. The seeds were shaken and soaked in 95% ethanol for 5 min. Then add sodium hypochlorite in 20% alcohol volume and Tween 20 in 0.1% alcohol volume, and continue soaking for 20 minutes. After pouring off the liquid, use sterile ddH 2 O Wash the seeds 3-5 times. Sow the seeds evenly on the glass petri dish containing 1 / 2 MS medium, seal i...

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Abstract

The invention discloses a tissue culturing method of physalis angulata. The tissue culturing method comprises following steps: seed sterile germination, callus induction, callus differentiation, rooting culturing, and hardening-seedling and transplanting. The tissue culturing method is adopted for tissue culturing of physalis angulata, is high in reproductive speed, and large in reproduction coefficient, and is not limited by seasons; obtained seedlings are uniform; and the excellent characteristics of an original variety can be maintained.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of bitter gourd. Background technique [0002] Physalis angulata L., also known as Lantern bulb and Lantern grass, is an annual herb of Physalis genus Solanaceae. It is distributed in East China, Central China, South China and Southwest China; it is also found in Japan, India, Australia and America. It often grows in valley forests and village side roadsides at an altitude of 500-1500 meters. The fruit, root or whole plant is used as medicine. It is bitter and cold in nature and taste. It is mainly used for sore throat, mumps, acute and chronic bronchitis, lung abscess, dysentery, dysuria, and external use for impetigo. Studies have found that solanaceae mainly contain chemical components such as withanolide, physalis, flavonoids, alkaloids and sterols, among which physalis and sitosterol have various anti-cancer activities in vivo and in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 邱胜卢江杰王慧中史钰军
Owner HANGZHOU NORMAL UNIVERSITY
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