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Application of SET gene in preparing product for diagnosing and/or treating gastric cancer

A gene and gastric cancer technology, applied in the fields of biotechnology and medicine, can solve the problems of insufficient research on the mechanism of gene and protein expression and function regulation

Inactive Publication Date: 2017-03-22
HUBEI UNIVERSITY OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

I2PP2A is also known as SET (patient SE translocation), and the research on the mechanism of its gene and protein expression and function regulation is not yet rich.

Method used

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  • Application of SET gene in preparing product for diagnosing and/or treating gastric cancer
  • Application of SET gene in preparing product for diagnosing and/or treating gastric cancer
  • Application of SET gene in preparing product for diagnosing and/or treating gastric cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 High expression of SET in gastric cancer cell lines

[0036] 1-1. Fluorescent quantitative PCR detection of high expression of SET in gastric cancer cell lines

[0037] Fluorescent quantitative PCR was used to detect the expression levels of SET genes in four human gastric cancer cell lines MKN28, MGC803, BGC823, and SGC7901 and normal gastric mucosal epithelial cells GES-1 cells, and found that the two cell lines BGC823 and SGC7901 highly expressed SET (see figure 1 A). Experiments were repeated three times. Among them, the fluorescent quantitative PCR detection uses β-actin as the internal reference gene, and the PCR primer sequence of the fluorescent quantitative PCR detection internal reference gene is:

[0038]β-actin forward primer 5'-CTCCTCCTGAGCGCAAGTACTC-3';

[0039] β-actin reverse primer 5'-TCCTGCTTGCTGATCCACATC-3'.

[0040] The primers for detecting the expression level of SET gene by fluorescent quantitative PCR are:

[0041] SET gene forward...

Embodiment 2

[0047] Example 2 High expression of SET protein level in gastric cancer tissue

[0048] Immunohistochemistry (IHC) analysis and evaluation:

[0049] Gastric cancer tissue samples and paracancerous tissue samples from patients undergoing gastrectomy who were admitted to Dongfeng General Hospital of Hubei University of Medicine were collected as the research objects. All cases were followed up by telephone or in-hospital reexamination. The complete survival period was calculated from the operation date to the date of last follow-up or date of death. Gastric cancer, paracancerous and normal tissues were obtained with the approval of the ethics committee and the informed consent of the patients. The collected gastric cancer specimens were all pathologically diagnosed and had complete follow-up data, and did not receive any local or systemic treatment before operation. Combined with the purchase of gastric cancer tissue chips, 102 cases of gastric cancer and 22 cases of normal ga...

Embodiment 3

[0052] Example 3 High expression of SET in gastric cancer is correlated with high invasion and poor prognosis

[0053] Statistical analysis: SPSS software package was used for statistical analysis. Correlation analysis was used to study the correlation between SET expression and clinicopathological parameters and overall survival in 102 gastric cancer samples in Example 2; the Kaplan-Meier method was used to compare the prognostic differences between groups with different expression of SET in the above samples.

[0054] Result analysis:

[0055] The clinicopathological and prognostic significance of SET protein expression in patients with gastric cancer were detected by correlation analysis, Kaplan-Meier method and Cox regression analysis. High SET protein expression levels were significantly correlated with highly aggressive tumor phenotypes (such as pathological grade, depth of invasion, lymph node stage, etc.) (P image 3 ). Table 1. Relationship between SET expression lev...

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Abstract

The invention belongs to the fields of biotechnology and medicine, and in particular relates to an application of an SET gene in preparing a product for diagnosing and / or treating gastric cancer. It is provided that the SET achieves frequent high expression (80%) in gastric cancer tissues, and the increase in SET expression is significantly correlated to aggressive phenotype and worse prognosis of a patient with the gastric cancer (P is less than 0.05). The low expression of the SET can obviously inhibit the migration and the invasion of gastric cancer cells. In addition, the SET, by virtue of a PP2A / Akt signaling pathway, can promote tumor malignant transformation. Furthermore, the gastric cancer tissues are obviously higher than para-carcinoma tissues in SET protein level. Therefore, the SET gene can serve as a specific marker gene for diagnosing the gastric cancer, so that it is more accurate and rapid to diagnose the gastric cancer. The SET gene provided by the invention provides a novel therapeutic target for the prevention and the treatment of the gastric cancer.

Description

technical field [0001] The invention belongs to the fields of biotechnology and medicine, and specifically relates to the application of a SET gene in the preparation of a product for diagnosing and / or treating gastric cancer. Background technique [0002] Gastric cancer is one of the most common cancers in East Asia (including China, Japan, and Korea) and Eastern Europe. The incidence and mortality of gastric cancer have been greatly reduced in the past half century. In developed countries, it remains one of the cancers with the highest mortality rates. In China, about two-thirds of gastric cancer patients will develop metastasis, and >50% of patients are at risk of recurrence after treatment. Metastasis and recurrence are important causes of patient death. Protein phosphatase 2A (protein phosphatase 2A, PP2A) is a serine / threonine protein phosphatase that plays an important role in a variety of cellular processes, including protein synthesis, signal transduction, cyc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68G01N33/577G01N33/574
CPCC12Q1/6886G01N33/57446G01N33/577G01N33/68G01N2333/475
Inventor 刘莹钦闪闪季娟丽张云飞张亮孙智婷
Owner HUBEI UNIVERSITY OF MEDICINE
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