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Identification and application of cotton drought-resistance related gene GhDRP1

A cotton and gene technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as functional redundancy

Inactive Publication Date: 2017-03-22
HUAZHONG NORMAL UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The amino acid sequence of the protein encoded by ABI2 and ABI1 has high homology, and the C-terminal region containing the core region of PP2C has 86% homology, and the non-conserved N-terminal region also has 48% homology, but ABI1 The N-terminal extension contains a Ca 2+ The EF-hand domain of the binding site, while the N-terminal extension of ABI2 does not contain the EF-hand domain, suggesting that there may be functional redundancy between the two

Method used

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  • Identification and application of cotton drought-resistance related gene GhDRP1
  • Identification and application of cotton drought-resistance related gene GhDRP1
  • Identification and application of cotton drought-resistance related gene GhDRP1

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Embodiment Construction

[0034] Cloning identification and functional analysis of the full-length sequence of a new cotton PP2C family gene GhDRP1:

[0035] 1. Isolation and identification of GhDRP1 gene and cDNA sequence

[0036] Using RNA-seq technology to analyze the transcriptomes of two cotton varieties (Jinmian 13 and Lumian 6), a drought-induced gene GhDRP1 was identified through comparative analysis of DNA and protein sequences. Then, Jinmian No. 13 cotton was used as the material to isolate and purify the total RNA of leaves, and then isolate and obtain the full-length cDNA sequence of GhDRP1, as shown in SEQ ID NO.2, with a total length of 1254bp. Primers were designed according to the GhDRP1 cDNA sequence, and the cotton genomic DNA was used as a template to amplify the full-length DNA sequence of the gene by PCR technology. As shown in SEQ ID NO.1, the total length is 1873bp, which includes 4 exons and 3 introns Introns (underlined), the first intron is from 496-602bp, a total of 107bp, t...

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Abstract

The invention discloses identification and an application of a cotton drought-resistance related gene GhDRP1. The invention provides the novel gene GhDRP1 from a cotton PP2C protein phosphatase family, and the expression of the gene is induced in the case of cotton drought stress. A gene sequence of the GhDRP1 consists of 3 introns and 4 exons. A gene coding region of the GhDRP1 is 1254bp long, and a protein containing 417 amino acids is encoded. The GhDRP1 protein is located in a cell nucleus. In the case of the cotton drought stress, the gene expression is obviously increased, showing that the gene is a drought inducing gene. Experiments show that the drought resistance of cotton can be weakened through the over-expression of the GhDRP1, and the drought resistance of the cotton can be enhanced by inhibiting the expression of the GhDRP1. In conclusion, the invention provides the functional gene which has an important application value in improving the drought resistance of the cotton.

Description

[0001] Technical field: [0002] The invention relates to the cloning and functional identification of a cotton drought resistance related gene GhDRP1. [0003] Background technique: [0004] Plant growth and development are closely related to the natural environment. Drought, floods, heat, frost, salinity, pests, weeds, etc. will have adverse effects on plant growth and development, often seriously affecting the yield and quality of crops (such as rice, wheat, cotton, etc.), and some data show that 50% of the The reduction in crop yield is all caused by abiotic stress. Water plays a huge role in the life activities of plants, and any solute must be dissolved in water before it can be absorbed and utilized. It is estimated that more than half of the crop production reduction caused by adverse environmental factors in the world is caused by drought and water shortage. [0005] Cotton is the most important fiber crop in the world. It belongs to the Malvaceae genus Gossypium and...

Claims

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Application Information

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IPC IPC(8): C12N15/55C12N9/16
CPCC12N9/16C12Y301/03016
Inventor 李学宝陈云刘致浩魏宁
Owner HUAZHONG NORMAL UNIV
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