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Method for preparing antibacterial membrane by N-succinyl-chitosan immobilized lysozyme

A technology of succinyl chitosan and lysozyme is applied in the field of preparing antibacterial film with modified chitosan solid-loaded lysozyme, and in the field of preparing antibacterial film with N-succinyl chitosan solid-loaded lysozyme, which can solve the problem of chitosan The problem of poor water solubility of sugar, etc., achieves the effect of smooth surface, good antibacterial property, and easy operation.

Active Publication Date: 2017-03-22
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention is to solve the poor water-solubility problem of conventional chitosan, therefore, prepare N-succinylphthalein chitosan by modification

Method used

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  • Method for preparing antibacterial membrane by N-succinyl-chitosan immobilized lysozyme
  • Method for preparing antibacterial membrane by N-succinyl-chitosan immobilized lysozyme
  • Method for preparing antibacterial membrane by N-succinyl-chitosan immobilized lysozyme

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Weigh 3g of chitosan powder and add it to 63mL of DMSO for mixing, slowly add 7.5g of succinic anhydride, react at 60°C for 7h, filter the solution, dissolve the precipitate in ethanol at room temperature, and react at room temperature for 2h. Adjust the pH of the solution to 10 with 1M NaOH, filter, and dissolve the precipitate in 140 mL of distilled water. Then add 420 mL of acetone, wash the precipitate three times with ethanol and acetone respectively, and dry the obtained product under vacuum condition at 50° C. for 24 h.

[0026] Weigh 1.0g NSC and dissolve it in 20mL citrate phosphate buffer with pH=5, add 0.02g EDC and NHS respectively, stir at room temperature for 2h, then add 0.05g lysozyme, stir for 2h, then add 7% glycerol , using the salivation method to make a film, dry it at 30°C for 7 hours, and peel off the film. Its antibacterial activity was tested by antibacterial test.

[0027] It was measured that the corresponding 2500ug / mL and 1250ug / mL lysozym...

Embodiment 2

[0029] Weigh 3g of chitosan powder and add it to 63mL of DMSO for mixing, slowly add 7.5g of succinic anhydride, react at 60°C for 7h, filter the solution, dissolve the precipitate in ethanol at room temperature, and react at room temperature for 2h. Adjust the pH of the solution to 10 with 1M NaOH, filter, and dissolve the precipitate in 140 mL of distilled water. Then add 420 mL of acetone, wash the precipitate three times with ethanol and acetone respectively, and dry the obtained product under vacuum condition at 50° C. for 24 h.

[0030] Weigh 1.0g NSC and dissolve it in 20mL citrate phosphate buffer with pH=6, add 0.02g EDC and NHS respectively, stir at room temperature for 2h, then add 0.05g lysozyme, stir for 2h, then add 7% glycerol , using the salivation method to make a film, dry it at 30°C for 7 hours, and peel off the film. Its antibacterial activity was tested by antibacterial test.

[0031] It was measured that the membrane solution with 2500ug / mL and 1250ug / m...

Embodiment 3

[0033] Weigh 3g of chitosan powder and add it to 63mL of DMSO for mixing, slowly add 7.5g of succinic anhydride, react at 60°C for 7h, filter the solution, dissolve the precipitate in ethanol at room temperature, and react at room temperature for 2h. Adjust the pH of the solution to 10 with 1M NaOH, filter, and dissolve the precipitate in 140 mL of distilled water. Then add 420 mL of acetone, wash the precipitate three times with ethanol and acetone respectively, and dry the obtained product under vacuum condition at 50° C. for 24 h.

[0034] Weigh 1.0g NSC and dissolve it in 20mL citrate phosphate buffer with pH=7, add 0.02g EDC and NHS respectively, stir at room temperature for 2h, then add 0.05g lysozyme, stir for 2h, then add 7% glycerol , using the salivation method to make a film, dry it at 30°C for 7 hours, and peel off the film. Its antibacterial activity was tested by antibacterial test.

[0035] It was measured that the membrane solution with the corresponding adde...

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Abstract

The invention discloses a method for preparing an antibacterial membrane by N-succinyl-chitosan immobilized lysozyme, and especially designs a natural antibacterial membrane with relatively strong water solubility. The method comprises the following steps: performing succinylation on chitosan to prepare NSC; activating the carboxyl of NSC through EDC and NHS; performing covalent binding with lysozyme; and finally, preparing the antibacterial membrane through a salivation method. The method is simple and easy to operate, and the prepared membrane has a relatively strong antibacterial property for staphylococcus aureus and listeria monocytogenes.

Description

technical field [0001] The invention designs a method for preparing an antibacterial film by immobilizing lysozyme on N-succinyl chitosan, in particular a method for preparing an antibacterial film by immobilizing lysozyme on modified chitosan. It belongs to the field of fine chemical technology. Background technique [0002] Chitin is the second largest type of biological material after cellulose in nature, and it is also the largest amount of nitrogen-containing natural organic matter on the earth except protein. The annual biosynthesis of chitin in nature is estimated to be as many as billions of tons, far more than other amino polysaccharides, and it is an inexhaustible renewable resource. People find that they have many application values ​​in many fields such as textile, printing and dyeing, papermaking, medicine, food, biology, chemical industry, agriculture and so on. Chitosan is the product of deacetylation of chitin macromolecules. It can be dissolved in acidic m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J5/18C08B37/08C08L5/08
CPCC08B37/003C08J5/18C08J2305/08C08L5/08C08L2203/16
Inventor 牛效迪席丽娟王虹苏吕卓陈洋
Owner JILIN UNIV
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