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Phosphorylated nfat3 mutants and their applications

A mutation and phosphorylation technology, which is applied in the fields of genetic engineering technology, biomedicine, molecular biology, and skin cancer detection, can solve problems such as unclear molecular mechanism, affecting treatment and prevention, etc.

Active Publication Date: 2019-12-24
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the exact molecular mechanisms of skin cancer development remain unclear, which seriously affects the treatment and prevention of the disease

Method used

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  • Phosphorylated nfat3 mutants and their applications
  • Phosphorylated nfat3 mutants and their applications
  • Phosphorylated nfat3 mutants and their applications

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Embodiment Construction

[0018] The present invention will be further described below through specific embodiments in conjunction with the accompanying drawings.

[0019] The methods used in the following embodiments are conventional methods unless otherwise specified, and the specific steps can be found in "Molecular Cloning: A Laboratory Manual" (Sambrook, J., Russell, David W., 3rdedition, 2001, NY, Cold Spring Harbor ). The DNA primers used were all synthesized by Invitrogen; NFAT3 expression plasmid pEGFP-C1-NFAT3 was purchased from Addgene; various restriction endonucleases were purchased from TaKaRa; reverse transcriptase was purchased from Life Technologies; point mutation kits were purchased from Agilent Technologies; Both DMEM medium and fetal bovine serum were purchased from HyClone Company; the transfection reagent Lipofectamine was purchased from Invitrogen Company; the melanoma cell culture medium was DMEM medium containing 10% fetal bovine serum. Human skin tissue chips were purchased ...

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Abstract

A phosphorylated NFAT3 mutant and an application thereof are disclosed. The number 259 site of NFAT3 is mutated from Ser into Ala. Phosphorylation of the Ser259 site of the NFAT3 influences skin cancer cell propagation, has anticancer application value, and can be used for NFAT3-targetted medicine research and development and skin cancer detection.

Description

technical field [0001] The invention relates to the fields of molecular biology, genetic engineering technology and biomedicine, in particular to the field of skin cancer detection. Background technique [0002] Skin cancer is one of the most common clinical malignant tumors, which can be roughly divided into malignant melanoma and non-melanoma skin cancer, the latter mainly including squamous cell carcinoma and basal cell carcinoma. Severe cases of skin cancer can be life-threatening, and its incidence is increasing year by year, which has become a global public health problem. Multiple risk factors can contribute to the development of skin cancer, including endogenous factors (genetic factors, gene mutations, etc.) and exogenous factors (such as ultraviolet exposure, chemical carcinogens, and other environmental stressors). Early diagnosis and treatment can reduce the death and deformity caused by the disease. However, the exact molecular mechanism of skin cancer remains...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47G01N33/574A61K38/17A61P35/00
CPCA61K38/00C07K14/4702G01N33/5743G01N2333/4703
Inventor 郑多肖田朱江何世平
Owner SHENZHEN UNIV
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