Application of thymol to preparation of medicines for treating salmonella infection

A Salmonella and Thymol technology, applied in the field of medicine, can solve the problem that Thymol has no anti-Salmonella infection, etc.

Inactive Publication Date: 2017-03-22
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report that thymol can resist Salmonella infection by inhibiting the function of Salmonella type Ⅲ secretion system at home and abroad.

Method used

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  • Application of thymol to preparation of medicines for treating salmonella infection
  • Application of thymol to preparation of medicines for treating salmonella infection
  • Application of thymol to preparation of medicines for treating salmonella infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] 1. Screening of Salmonella type III secretion system inhibitors

[0024] β-lactamase-SipA fusion and its fluorescent substrate CCF-4 were used to detect the effect of test compounds on the function of type Ⅲ secretion system.

[0025] 2. The effect of thymol on the growth of Salmonella

[0026] Cultivate Salmonella in LB (containing 0.3M NaCl) liquid medium overnight (37°C, 200rpm), expand the culture to OD at 1:100 600nm The value is about 0.1, and the positive group and the drug treatment group with different concentrations are set respectively. Continue to cultivate, and measure the optical density value of each sample every 30 minutes until the plateau. The experimental results showed that thymol appeared to be sub-inhibitory at a drug concentration of 0.6mM, but it did not affect the growth of bacteria at a drug concentration of 0.4mM. (see attached figure 1 ).

[0027] 3. Verify that thymol inhibits the secretion function of type Ⅲ secretion system

[0028] ...

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Abstract

The invention provides an application of thymol to preparation of medicines for treating salmonella infection. By using a human cervical carcinoma cell (Hela) as an infection carrier, gentamicin protective experiments, in vitro biochemical experiments and mouse salmonella infection models all verify that thymol has obvious inhibiting effects on salmonella infection by affecting the functions of secretory systems III. Antibiotics easily induce generation of medicine resistance in the process of treating salmonella infection. Compared with the antibiotics, the traditional Chinese medicine compound thymol can directly inhibit the functions of the salmonella secretory systems III and play a role in resisting infection, does not easily induce generation of medicine resistance and has the characteristic of high cure rate.

Description

technical field [0001] The invention relates to the application of thymol in the preparation of medicines for treating Salmonella infection, belonging to the field of medicine. Background technique [0002] Salmonella is one of the most important food-borne pathogens causing food poisoning in humans and animals, and it is also a zoonotic pathogen of great significance in global public health. The pathogen has a wide range of hosts in nature, most of which are adapted to humans and animals. Salmonella often causes serious diseases after infecting animals, mainly affecting young animals, causing typhoid fever, sepsis, gastroenteritis and local inflammation of other tissues, and can cause fatal infections such as abortion and stillbirth in pregnant female animals, among which the more serious harm is pullorum , swine septicemia, enteritis and paratyphoid fever have brought serious economic losses to the livestock and poultry industry, and even seriously threatened public healt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/05A61P31/04
CPCA61K31/05Y02A50/30
Inventor 邓旭明罗招庆张勇刘雁冯海华王建锋
Owner JILIN UNIV
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