Preparation method of Nitraria tangutorum Bobr. antioxidant active extract, and products and applications thereof
A technology of antioxidant activity and extraction method, which is applied in the field of preparation of Nitraria antioxidative activity extract, and can solve the problems of unsatisfactory antioxidant activity of the extract and low yield of the extract, etc.
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[0021] like figure 1 Shown, the present invention provides a kind of preparation method of Nitraria antioxidative activity extract, comprises the steps:
[0022] S1: Mix Nitraria dried fruit with ethanol aqueous solution with a volume fraction of 30% to 95% at a solid-liquid ratio of 1g:(5 to 30)mL, cold soak for 1h to 4h, and mix the Nitraria dried fruit with the ethanol aqueous solution The mixture is extracted, and the extraction method of the extraction is one or more of heating reflux, ultrasonic extraction, microwave extraction and flash extraction, and the extraction times of the flash extraction are 3 to 5 times, and the flash extraction Each extraction time of extraction is 1min~2min, the ultrasonic temperature of the ultrasonic extraction is 40°C, the ultrasonic time is 60min, and the range of solid-liquid ratio is preferably 1g:(5~30)mL, which is considering that the ratio is greater than 1g / mL is not conducive to the extraction of the extract. If the ratio is les...
Embodiment 1
[0029] This embodiment provides a preparation method of Nitraria antioxidative activity extract, comprising the following steps:
[0030] S1: 15g of Nitraria siberia dried fruit (produced in Qinghai, batch number QH2014) was mixed with 450mL of 30% ethanol water solution by volume fraction, cold soaked for 4 hours, and then the mixture of Nitraria thorn dried fruit and ethanol water solution was ultrasonically extracted, and the ultrasonic temperature was 40℃, ultrasonic time is 60min;
[0031] S2: filtering the extracted solution, concentrating the filtered filtrate to obtain an extract;
[0032] S3: Dissolve the concentrated product in water, and use the chromatographic column filled with AB-8 macroporous resin to absorb and separate the obtained solution. The mass ratio of the product to the macroporous resin is 1:100, and the time for adsorption and separation is 12 hours;
[0033] S4: elute the chromatographic column with water until the effluent is colorless, then elute...
Embodiment 2
[0036] This embodiment provides a preparation method of Nitraria antioxidative activity extract, comprising the following steps:
[0037] S1: 15g of Nitraria siberia dried fruit (produced in Qinghai, batch number QH2014) was mixed with 450mL of 50% ethanol water solution by volume fraction, cold soaked for 4 hours, and then the mixture of Nitraria thorn dried fruit and ethanol water solution was ultrasonically extracted, and the ultrasonic temperature was 40℃, ultrasonic time is 60min;
[0038] S2: filtering the extracted solution, concentrating the filtered filtrate to obtain an extract;
[0039] S3: Dissolve the concentrated product in water, and use the chromatographic column filled with AB-8 macroporous resin to absorb and separate the obtained solution. The mass ratio of the product to the macroporous resin is 1:100, and the time for adsorption and separation is 12 hours;
[0040] S4: elute the chromatographic column with water until the effluent is colorless, then elute w...
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