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Chinese fir stem tip tissue culture reproduction method

A tissue culture and shoot tip technology is applied in the field of fir shoot tip tissue culture and reproduction, which can solve the problems of low success rate of culture and immature technology, and achieve the effects of simple induction method and low induction cost.

Inactive Publication Date: 2017-03-15
阜阳市颍泉区玉寿种植专业合作社
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Problems solved by technology

Although the market for small-diameter timber has been sluggish in recent years, the sales of medium- and large-diameter timber are still good. In the future, we will mainly cultivate medium- and large-diameter Chinese fir. At present, there are many rapid propagation methods for relatively mature Chinese fir, mainly including sexual reproduction and asexual reproduction. , in tree production, sexual reproduction refers to cultivating seedlings through seeds, and asexual reproduction includes cultivating vegetative propagation seedlings through cuttings, root cuttings, layering, embedding, root tillers, leaf cuttings, grafting and tissue culture. In the process of fir tissue culture, the current technology is not very mature, so the success rate of culture is low

Method used

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Examples

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Effect test

Embodiment 1

[0015] Embodiment 1, a kind of Chinese fir stem tip tissue culture propagation method, comprises the following steps:

[0016] (1) Select the stem tip of Chinese fir as an explant, immerse the stem tip in an aqueous solution of silver iodide with a mass concentration of 2%, and treat it under ultrasonic waves with a power of 130W for 10 seconds;

[0017] (2) Place the sterilized shoot tips on a sand bed with a humidity of 85% after disinfection to induce callus formation, and evenly spray 3% mass concentration of berberine sulfate solution in the sand bed to adjust the humidity of the sand bed;

[0018] (3) Place the callus-forming stem tip at a temperature of 25°C and induce budding under a red light with a light intensity of 800Lx. During the germination period, spray the mist on the shoot tip surface once every 2 hours for 3 The basic fibroblast growth factor solution of % mass concentration;

[0019] (4) Induce rooting induction by immersing the base of the shoot apex of ...

Embodiment 2

[0020] Embodiment 2, a kind of Chinese fir stem tip tissue culture propagation method, comprises the following steps:

[0021] (1) Select the stem tip of Chinese fir as the explant, immerse the stem tip in a silver iodide aqueous solution with a mass concentration of 2.8%, and treat it for 12 seconds under an ultrasonic wave with a power of 140W;

[0022] (2) Place the sterilized shoot tips on a sand bed with a humidity of 90% after disinfection to induce callus formation, and evenly spray a 4% mass concentration of berberine sulfate solution in the sand bed to adjust the humidity of the sand bed;

[0023] (3) Place the callus-forming shoot tip at a temperature of 28°C and induce budding under red light with a light intensity of 1000 Lx. During the budding induction period, spray a mist on the shoot tip surface once every 2 hours 4 The basic fibroblast growth factor solution of % mass concentration;

[0024] (4) Induce rooting induction by immersing the base of the shoot apex...

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Abstract

The invention relates to the technical field of Chinese fir planting, in particular to a Chinese fir stem tip tissue culture reproduction method. The method comprises the following steps: dipping stem tips in silver iodide aqueous solution for performing ultrasonic sterilization, adding the stem tips into a sand bed at humidity regulated by berberine sulfate solution so as to induce to form callus, spraying basic fibroblast growth factor solution to induce germination under red light, and dipping in rooting induction liquid obtained by mixing a disodium hydrogen phosphate dihydrate solution and a zinc chloride solution according to a ratio of 3:1 for inducing rooting. According to the method disclosed by the invention, the Chinese fir stem tips serve as an explant for performing induction culture on Chinese fir seedlings, the induction cost is low, the induction method is simple, and large-scale batch induction can be realized, so that seedlings with roots and buds can be rapidly formed from the stem tips.

Description

technical field [0001] The invention relates to the technical field of Chinese fir planting, in particular to a method for tissue culture and propagation of Chinese fir stem tips. Background technique [0002] Chinese fir is a unique fast-growing commercial timber tree species in my country. It grows fast and has good materials. It is artificially cultivated in large quantities at the Dafeng fir base. The wood texture is straight, the structure is uniform, not warped or cracked, the material is light and tough, the strength is moderate, and the quality factor is high. It has a fragrance, and the wood contains "Shannao", which can resist insects and rot, and is easy to process. Widely used in construction, furniture, appliances, shipbuilding and other aspects. Although the market for small-diameter timber has been sluggish in recent years, the sales of medium- and large-diameter timber are still good. In the future, we will mainly cultivate medium- and large-diameter Chines...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 张玉李玉龙
Owner 阜阳市颍泉区玉寿种植专业合作社
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