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Method for culturing and propagating visible tiller tissues at connecting part of cedar stem

A technology of tissue culture and tissue culture medium, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low success rate of cultivation and immature technology, etc., achieve large quantity, low induction cost, and reduce pollution rate effect

Inactive Publication Date: 2017-03-15
阜阳市颍泉区玉寿种植专业合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Chinese fir, an important timber species in my country, has been cultivated for more than 100 years according to historical records, and has made outstanding contributions to my country's economic development. It grows fast and has good materials. It has been artificially cultivated in large quantities. There are many rapid propagation methods of Chinese fir, mainly including sexual reproduction and asexual reproduction. The vegetative propagation seedlings are cultivated by means of strips, root tillers, leaf cuttings, grafting and tissue culture. In the process of fir tissue culture, the current technology is not very mature, so the success rate of culture is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1, a kind of bright tiller tissue culture propagation method of Chinese fir stem junction, comprises the following steps:

[0013] (1) Sterilization of explants: select the 7-8-year-old Chinese fir root-stem junction as explants, immerse the explants in silver iodide aqueous solution with a mass concentration of 4.5%, and treat them under ultrasonic waves with a power of 160W 8 seconds;

[0014] (2) Induce callus formation: inoculate the sterilized explants into callus culture medium, and the composition of callus culture medium is MS medium + Moringa oleifera seed extract 3mg / L;

[0015] (3) Bud tissue induction: Inoculate the explants into the bud tissue culture medium, and the bud tissue culture medium is composed of MS medium + datura seed extract 1mg / L; during the bud tissue induction, the explanted The body was incubated at 18°C ​​for 10 hours, and then at 28°C for 30 hours;

[0016] (4) Root induction: the explants were artificially fixed and inocula...

Embodiment 2

[0018] Embodiment 2, a kind of bright tiller tissue culture propagation method of Chinese fir stem junction, comprises the following steps:

[0019] (1) Sterilization of explants: Select the bright tiller at the junction of the 7-8-year-old Chinese fir rhizome as explants, immerse the explants in silver iodide aqueous solution with a mass concentration of 5%, and use an ultrasonic wave with a power of 160-180W to sterilize the explants. Down processing for 10 seconds;

[0020] (2) Induce callus formation: inoculate the sterilized explants into callus culture medium, and the composition of callus culture medium is MS medium + Moringa oleifera seed extract 3.4mg / L;

[0021] (3) Bud tissue induction: the explants were inoculated into the bud tissue culture medium, and the bud tissue culture medium was composed of MS medium + datura seed extract 1.2 mg / L; during the bud tissue induction, the explants were first The implants were incubated at 20°C for 12 hours, and then at 30°C fo...

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PUM

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Abstract

The invention relates to the technical field of cedar planting, and in particular relates to a method for culturing and propagating visible tiller tissues at a connecting part of a cedar stem. The method comprises the following steps: performing ultrasonic sterilization on an explant in a silver iodide solution, inoculating the explant into an MS culture medium with a moringa oleifera extract to induce calluses, inoculating the explant into an MS culture medium with a stramonium seed extract to induce bud tissues, and performing rooting induction in a 3% glucosamine hydrochloride solution. As a visible tiller at the connecting part of the cedar stem is adopted as the explant to induce and culture cedar seedlings, the induction cost is low, the induction method is simple, large-scale batched induction can be implemented, and explants can grow into seedlings with roots and buds rapidly.

Description

technical field [0001] The invention relates to the technical field of Chinese fir planting, in particular to a method for tissue culture and propagation of fir stem joints. Background technique [0002] Chinese fir, an important timber species in my country, has been cultivated for more than 100 years according to historical records, and has made outstanding contributions to my country's economic development. It grows fast and has good materials. It has been artificially cultivated in large quantities. There are many rapid propagation methods of Chinese fir, mainly including sexual reproduction and asexual reproduction. Strips, root tillers, cuttings, grafting and tissue culture methods to cultivate vegetative propagation seedlings. In the process of fir tissue culture, the current technology is not very mature, so the success rate of culture is low. Contents of the invention [0003] The invention provides a method for tissue culture and reproduction of fir stem joints. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 张玉李玉龙
Owner 阜阳市颍泉区玉寿种植专业合作社
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