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Production method of Euonymus bungeanus leaf regenerated plant

A technology for regenerating plants of peach leaf eucalyptus, which is applied in the field of plant tissue culture, can solve the problems of strong time requirements for sowing and breeding seeds collection, complex pretreatment, and high management requirements, and achieves improvement of reproduction coefficient and survival rate, short cultivation period, and operation. simple method effect

Active Publication Date: 2017-03-15
金树林生态科技(南京)有限公司
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Problems solved by technology

[0005] The propagation of Euonymus peach leaf generally adopts methods such as sowing, cutting, and division propagation. However, due to the limitation of the number of seed gardens and the output, there are very few high-quality seedlings obtained, and the propagation speed is relatively slow. The time required for sowing and propagating seeds is very strong. The post-pretreatment is complicated and the post-management requirements are high. It is difficult to use the method of seed propagation to cultivate seedlings on a large scale in production. How to use the method of plant tissue culture to select high-quality seedlings has become the focus of attention in the process of Euonymus peach leaf propagation

Method used

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  • Production method of Euonymus bungeanus leaf regenerated plant
  • Production method of Euonymus bungeanus leaf regenerated plant
  • Production method of Euonymus bungeanus leaf regenerated plant

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Experimental program
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Embodiment Construction

[0025] 1. Selection of propagation material

[0026] The test materials were taken from two-year-old peach leaf Euonymus leaves at the Experimental Station of Jinling Institute of Technology.

[0027] 2. Disinfection of propagation material

[0028] Rinse the material with running water for 1 hour to remove surface debris. On the aseptic operating table, rinse with sterile water for 3 times, then sterilize with 75% absolute ethanol for 30 seconds, then rinse with sterile water for 3 times, and then use 0.1% Mercuric chloride disinfection treatment for 5-7min, and finally rinsed with sterile sterile water 4 times; see attachment figure 1 .

[0029] 3. Induction of callus

[0030] On the ultra-clean workbench, cut the sterilized leaves into 6mm squares and inoculate them on the callus induction medium. The back of the leaves are close to the medium. Inoculate 3-4 pieces per bottle. The induction light conditions are 14h / d. , Light intensity 2000lux, temperature 23-28℃; see attached fig...

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Abstract

The invention belongs to the technical field of plant tissue culture, and particularly relates to a production method of a Euonymus bungeanus leaf regenerated plant. The production method comprises the following steps: selection and disinfection of explant, induction of callus, induction of adventitious bud and induction of root. The method has the advantages of short culture period, fewer variations, artificially controllable culture conditions and simple operating process, and lays the foundation for seed breeding, genetic transformation, breed improvement and other researches of Euonymus bungeanus as an excellent scientific research material, thereby providing guarantees for large-area production of Euonymus bungeanus tissue culture seedlings.

Description

[0001] One technical field [0002] The invention belongs to the technical field of plant tissue culture, and specifically relates to a production method for regenerating plants from the leaves of Euonymus peach leaves. [0003] 2. Technical background [0004] Due to its good wood quality, high seed oil content, high ornamental value, and large plantation area, Euonymus peach has become one of the most promising garden tree species in the 21st century to improve the living environment of humans and meet the needs of human life. [0005] The propagation of Euonymus vulgaris generally uses methods such as sowing, cutting, and ramet propagation. However, due to the limitation of the number of seed gardens and yield, the quality of seedlings obtained is very small, the propagation speed is relatively slow, and the collection time of sowing and propagation seeds is very demanding. The post-pretreatment is complicated and the management requirements in the later stage are high. It is diffic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 马艳程馨慧尚静翟亚娇肖木株
Owner 金树林生态科技(南京)有限公司
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