A kind of polydopamine-coated hemoglobin oxygen carrier and preparation method and application thereof
A technology of hemoglobin and polydopamine, which is applied in the field of blood substitutes of hemoglobin oxygen carriers, can solve problems such as complex reaction systems, and achieve the effects of simple synthesis process, good blood compatibility, and high oxygen affinity
Active Publication Date: 2019-12-17
ACADEMY OF MILITARY MEDICAL SCI
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Problems solved by technology
It can be seen that the reaction systems of the double emulsion method and the nano-deposition method are relatively complicated, and both need to remove organic solvents through cumbersome operations such as rotary evaporation or extraction.
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[0060] A series of polydopamine-coated hemoglobin oxygen carriers are prepared according to the above method, as shown in Table 1; The more concentrated the particle size of the nanoparticles, the more uniform the dispersion) and the P50 value of the oxygen carrier.
[0061] Table 1 Example polydopamine encapsulated parameters of hemoglobin oxygen carrier
[0062]
[0063] It can be seen from the data in Table 1 that the polydopamine-coated hemoglobin oxygen carrier provided by the present invention has an average particle size distribution of 6nm-16nm, the polydispersity index PDI of the oxygen carrier is 0.15-0.70, and the P50 value of the oxygen carrier is 11mmHg-17mmHg. Among them, the average particle size distribution of the oxygen carrier is preferably 6.2-15.3nm, more preferably 6.9nm-11.5nm, most preferably 7.4nm; the polydispersity index PDI of the oxygen carrier is preferably 0.154-0.664, more preferably 0.179-0.331, most preferably 0.243.
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Abstract
The invention discloses a hemoglobin-based oxygen carrier wrapped by polydopamine as well as a preparation method and applications of the hemoglobin-based oxygen carrier. The hemoglobin-based oxygen carrier is obtained by wrapping hemoglobin by adopting polydopamine. Specifically, the preparation method comprises the following steps: mixing hemoglobin stock solution and dopamine solution, and carrying out polymerization reaction of dopamine and the loading reaction of wrapping hemoglobin by adopting polydopamine, thus obtaining the hemoglobin-based oxygen carrier wrapped by polydopamine. The oxygen carrier provided by the invention belongs to the nanoscale, and is free of the agglomeration phenomenon; hemolysis can not be caused, and the cytotoxicity can not be caused; the chemical constitution and secondary structure of hemoglobin can not be damaged, and thus the normal exertion of the physiological function of hemoglobin is guaranteed; the oxygen loading and release can be effectively realized, the oxygen affinity is high, meanwhile, the oxidation resistance is good, the auto-oxidation of hemoglobin can be effectively inhibited, the various free radicals and active oxygen in red blood cells can be eliminated, and the problem that the existing hemoglobin carriers easily have oxidative damage is solved. The preparation process is simple, the consumed time is short, and the method is suitable for large-scale production.
Description
technical field [0001] The invention relates to the technical field of blood substitutes of hemoglobin oxygen carriers, in particular to a polydopamine-coated hemoglobin oxygen carrier and its preparation method and application. Background technique [0002] As an important means of life-saving, blood transfusion is widely used in clinical operations, disaster relief and war wound treatment. However, due to the shortage of blood sources, the short storage period of blood, and the spread of blood-borne diseases, traditional blood transfusion techniques are increasingly unable to meet the needs of emergency situations. Therefore, the synthesis of safe and effective blood substitutes that can be produced on a large scale has always attracted the attention of researchers. [0003] Whether it can simulate the oxygen-carrying function of red blood cells is an important criterion for evaluating the effectiveness of blood substitutes. This function is achieved through the dynamic ...
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IPC IPC(8): A61K38/42A61K9/50A61K47/34A61P7/08
CPCA61K9/5031A61K38/42
Inventor 周虹赵莲王权胡吉林卢明子吴雁张瑞锐王瑛尤国兴尹玉静陈赣
Owner ACADEMY OF MILITARY MEDICAL SCI
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