Acetylglucosaminidasedetection reagent, reaction pad, preparation method of reaction pad and kit

A technology of glucosidase and acetylamino, which is applied in the field of acetylglucosaminidase detection reagents, can solve problems such as lack of accuracy, and achieve the effects of good accuracy, high efficiency, and simple and convenient use conditions

Inactive Publication Date: 2017-02-22
GUANGZHOU HONGQI OPTICAL INSTR TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection reagents currently used to detect acetylglucosaminidase are not accurate enough

Method used

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  • Acetylglucosaminidasedetection reagent, reaction pad, preparation method of reaction pad and kit
  • Acetylglucosaminidasedetection reagent, reaction pad, preparation method of reaction pad and kit
  • Acetylglucosaminidasedetection reagent, reaction pad, preparation method of reaction pad and kit

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0047] The preparation method of this reaction pad comprises the following steps:

[0048] 1) Material preparation: prepare anhydrous methanol, p-nitrobenzene-N-acetylglucosaminidase, polyvinylpyrrolidone, Triton X-100, EDTA and citric acid according to the above parts by weight;

[0049] 2) Prepare soaking solution A: fully dissolve p-nitrobenzene-N-acetylglucosaminidase in anhydrous methanol to obtain soaking solution A;

[0050] 3) Prepare soaking solution B: dissolve polyvinylpyrrolidone, Triton X-10, EDTA and citric acid in pure water to obtain soaking solution B;

[0051] 4) Soaking solution A treatment: put the filter paper in the soaking solution A and fully soak it for 10-20 seconds, then take it out, and then dry it at a temperature of 20-70°C for 30-40 minutes to obtain a filter paper of the soaking solution A;

[0052] 5) Soaking solution B treatment: soak the filter paper of soaking solution A in soaking solution B for 10-20 seconds, take it out, and then dry it ...

Embodiment 1～3

[0059]Embodiments 1-3 disclose a kind of acetylglucosaminidase detection reagent, respectively prepare three groups of raw material components according to Table 1, and prepare sodium hydroxide solutions with different concentrations according to Table 2 as chromogen; Group A is used in Example 1 , Group B is used in Example 2, and Group C is used in Example 3.

[0060] Dissolve anhydrous methanol, p-nitrophenyl-N-acetylglucosaminidase, polyvinylpyrrolidone, Triton X-10, EDTA and citric acid in 100mL of pure water in sequence to obtain a reactant; , to obtain coagulase detection reagent.

[0061] The composition and content of table 1 reactant

[0062]

[0063] The preparation parameter of table 2 chromogen

[0064]

[0065] Detect the detection reagents obtained in Examples 1 to 3: prepare acetylglucosaminidase standard solution with different enzyme activity concentrations respectively, and the acetylglucosaminidase standard solution uses physiological saline as a so...

Embodiment 4～6

[0072] Embodiment 4~6 discloses a kind of kit:

[0073] 1) Prepare materials according to Table 1 and Table 2; Group A is used in Example 4, Group B is used in Example 5, and Group C is used in Example 6;

[0074] 2) Prepare soaking solution A: fully dissolve p-nitrobenzene-N-acetylglucosaminidase in anhydrous methanol to obtain soaking solution A;

[0075] 3) Prepare soaking solution B: dissolve polyvinylpyrrolidone, Triton X-10, EDTA and citric acid in pure water to obtain soaking solution B;

[0076] 4) Soaking solution A treatment: put the filter paper in the soaking solution A and fully soak it for 10-20 seconds, then take it out, and then dry it at a temperature of 40°C for 40 minutes to obtain a filter paper of the soaking solution A;

[0077] 5) Treatment of soaking solution B: put the filter paper of soaking solution A into soaking solution B for 10-20 seconds, take it out, and then dry it at a temperature of 45°C for 30 minutes to obtain a reaction pad;

[0078] 6)...

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Abstract

The invention discloses an acetylglucosaminidasedetection reagent, a reaction pad, a preparation method of the reaction pad and a kit. The acetylglucosaminidasedetection reagent comprises a reactant and a color-developing agent; the preparation method of the reaction pad includes the steps that 1, para-nitrobenzene-N-acetylglucosaminidasedetection is dissolved in absolute methanol, so that a soaking solution A is obtained; 2, polyvinylpyrrolidone, Triton X-10, EDTA and citric acid are dissolved in pure water, so that a soaking solution B is obtained; 3, a carrier is put into the soaking solution A to be soaked and then taken out to be dried, so that a soaking solution A carrier is obtained; 4, the soaking solution A carrier is put into the soaking solution B to be soaked and then taken out to be dried, so that the reaction pad is obtained. The reaction pad prepared through the method is combined with components of the reagent to be applied to preparation of the kit which is high in sensitivity and accuracy.

Description

technical field [0001] The invention relates to the field of acetylglucosaminidase detection reagents, in particular to an acetylglucosaminidase detection reagent, a reaction pad, a preparation method thereof and a kit. Background technique [0002] Vaginitis is an inflammation of the vaginal mucosa and submucosal connective tissue, and is a common disease in gynecological clinics. For normal healthy women, due to anatomical and biochemical characteristics, the vagina has a natural defense function against the invasion of pathogens. When the natural defense function of the vagina is destroyed, pathogens are easy to invade and cause vaginal inflammation. There are three types of common vaginitis: bacterial vaginosis (BV / AV), fungal vaginitis (VVC) and trichomonas vaginitis (TV). Among them, fungal vaginitis, also known as candidal vaginitis, is a type of vaginal inflammation that is caused by Candida, especially Candida albicans, as a pathogen. Trichomonal vaginitis is a ki...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/775G01N2021/7756
Inventor 眭红燕朱华琳范静彦李必松
Owner GUANGZHOU HONGQI OPTICAL INSTR TECH
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