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Method for rapidly detecting urine sugar and uric acid in solution

A solution and urine sugar technology, applied in measuring devices, material analysis by observing the influence of chemical indicators, instruments, etc., can solve the problems of expensive instruments, troublesome transportation, etc., and achieve low cost, fast operation, and easy operation Effect

Inactive Publication Date: 2017-02-22
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whether it is urine sugar or uric acid, these existing analysis methods require professionals to operate, expensive instruments, and troublesome transportation. Therefore, a fast and simple analysis method for the determination of uric acid content is needed to make up for the gaps in the existing technology

Method used

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  • Method for rapidly detecting urine sugar and uric acid in solution
  • Method for rapidly detecting urine sugar and uric acid in solution
  • Method for rapidly detecting urine sugar and uric acid in solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] First, the desktop printer is modified, and the hydrophobic material alkyl ketene dimer (AKD) is formulated into a solution (AKD: n-heptane = 2g: 25ml), which is added dropwise into the ink cartridge for printing. At the same time, use Word to design the detection area of ​​the paper chip. The outline of the paper chip is printed first, followed by the hydrophobic areas. Finally, put the printed filter paper in an oven at 98°C for about 5 minutes, so that AKD is more tightly bound to the surface of the filter paper to form hydrophobic channels ( figure 1 ). The paper chip obtained by this method can be loaded with 3-5 μL of reagents, effectively ensuring that the reagents used for detection do not leak out of the detection area.

Embodiment 2

[0042] (1) Add the following reagents and sample solutions in sequence on the paper chip: glucose oxidase enzyme activity is 120U / mL, dosage 0.6μL; horseradish peroxidase enzyme activity is 80U / mL, dosage 0.6μL; concentration of potassium iodide solution 0.6mol / L, the dosage is 1.0μL; the urine glucose standard solution concentration gradient is 2.0, 4.0, 6.0, 8.0, 10.0g / L, and the glucose sample solution with a concentration of 3.0g / L is used to verify the standard curve to determine the error of the actual sample , the dosage is 1.0 μL.

[0043] (2) Load each reagent and urine sugar solution according to the above sequence, and react for about 10 minutes to fully develop the color ( figure 2 ), take pictures of the paper chip, measure the RGB color intensity values ​​corresponding to different concentrations of urine sugar solutions with imageJ software, make a linear curve between the intensity value and the concentration of the standard solution, and draw the correlation ...

Embodiment 3

[0045] (1) Add 1.0 μL of 4mol / L 4-aminoantipyrine (4-AAP) and 1.0 μL of 8mol / L 3,5-dichloro-2-hydroxy-benzenesulfonate (DHBS) to the paper chip in sequence , Enzyme activity unit 80U / mL uric acid oxidase (UOD) 1.0μL, enzyme activity unit 339U / mL horseradish peroxidase (HRP) 1.0μL, dry the area where the reagent is to be loaded until no trace of water is visible;

[0046] (2) Add different concentrations of uric acid standard solutions and sample solutions to the paper chip detection area of ​​the four reagents, fully react, and wait for the color to appear ( image 3 );

[0047] (3) Take a picture with a mobile phone and use imageJ software to measure the RGB color intensity of the color area, and bring it back to the standard curve to calculate the concentration of uric acid in the solution.

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Abstract

The invention relates to a method for rapidly detecting urine sugar and uric acid in a solution. According to a method, a printer is adopted, paper AKD is used as a hydrophobic material, a paper chip is made on a piece of filter paper to serve as a detection carrier. Glucose oxidase and horse radish peroxidase are used as catalysis reagents for urine sugar detection, and potassium iodide is used as a coloration reagent. The uric acid detection is performed by adopting a UOD-POD two-enzyme method. The reagents are supported in a detection zone on the paper chip. After the chip is completely dried, standard urine sugar and uric acid solutions or sample solutions different in concentration are detected, photographing is performed, pictures are processed to obtain RGB color intensity values, and standard curves about the RGB color intensity values and the concentrations of the standard solutions are established. The method utilizes high selectivity of enzymatic reaction, combines the simple and convenient paper chip and the visual properties and high sensitivity of multielement detection and chemiluminescence imaging, fully plays the advantages of the three parts, and has the advantages of being low in cost, simple in operation, rapid, easy to operate and the like.

Description

technical field [0001] The invention relates to the field of sample detection in solution, in particular to a method for detecting urine sugar and uric acid. Background technique [0002] Glucose is an indispensable substance in life activities, and its level directly affects people's physical condition. When the blood flows through the kidneys, the glucose in it is filtered into the renal tubules through the glomeruli, and most of the glucose in the renal tubules is reabsorbed into the human blood. There is only a small amount of glucose in the urine, which cannot be detected by ordinary methods , can be said to be sugar-free. In normal people, only when the blood sugar exceeds 160-180mg / dl, more sugar can be excreted from the urine to form urine sugar. Urine glucose test is the easiest way to diagnose diabetes early. [0003] Uric acid is produced by the oxidation of nucleic acids to purines, which are then oxidized again in the liver. The normal content of uric acid i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/7759
Inventor 王盈悦董建博胡雅雯丁佳琦张敏胡坪
Owner EAST CHINA UNIV OF SCI & TECH
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