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Lysobacter enzymogenes 1-T-1-4 and application thereof

A technology for producing lysobacterium and lipase, which is applied in the direction of application, bacteria, and fungicides, can solve the problems of mutagenesis, reinfestation, and carcinogenesis, and achieve reduced usage and residual amounts, high-efficiency control effects, and reduced production cost effect

Inactive Publication Date: 2017-02-22
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2014, the planting area of ​​Panax notoginseng in Yunnan Province exceeded 600,000 mu, and its economic value was equivalent to the output value of 20 million mu of corn. The current agricultural output value and the total output value of Panax notoginseng with Panax notoginseng as raw material in the country exceeded 70 billion yuan. The most important pillar species in the traditional Chinese medicine industry, but the production of Panax notoginseng has serious continuous cropping obstacles. Due to the large-scale single planting in successive years, and the fact that Panax notoginseng is a perennial herb that likes warmth and shade, the continuous cropping obstacle is particularly prominent.
After the konjac plant is infected with soft rot, the leaves or bulbs become soft, black and rotten, and have a foul smell. The fungal juice flows out from the diseased part and infects new plants, making it diseased. The loss is generally 30%-50%, and in severe cases, it can reach 80% or even no harvest, which seriously affects the enthusiasm of taro farmers to plant konjac and hinders the rapid development of local economies in Southwest China
[0005] At present, agricultural cultivation measures, disease-resistant varieties and chemical control measures are mainly used to prevent and control the above diseases. Among them, chemical pesticide control is the most widely and frequently used. drug resistance) and chemical pesticides may have "three-caused" effects (causative, carcinogenic, and mutagenic). Although the pesticides contained in the soil do not directly endanger human health, they can be absorbed by crops. And humans will be more or less harmed by pesticides by ingesting these crops

Method used

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  • Lysobacter enzymogenes 1-T-1-4 and application thereof
  • Lysobacter enzymogenes 1-T-1-4 and application thereof
  • Lysobacter enzymogenes 1-T-1-4 and application thereof

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Experimental program
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Effect test

Embodiment 1

[0032] Preparation of Lysobacter enzymogenes 1-T-1-4 Aqua

[0033] (1) Bacteria culture

[0034] a. test tube culture (below are weight percentages)

[0035] Put NA medium (5g of peptone, 10g of sucrose, 1g of yeast powder, 3g of beef extract, 17-20g of agar) into a test tube, make up to 1000mL with distilled water, sterilize, make a slope, and inoculate Lysobacter zymolyticum 1- T-1-4 strain, cultivated at 26°C for 24h.

[0036]b. Bacteria Shaker Expansion Cultivation

[0037] The slant seeds were inoculated on the eggplant bottle slant NA medium (the formula of the medium was the same as the above-mentioned step a), and cultured at 26° C. for 48 hours to obtain the eggplant bottle seeds.

[0038] (2) fermentation

[0039] The specific fermentation production process is as follows:

[0040] a. Preparation of seed suspension: wash the bacterial lawn on the slope of the eggplant bottle with sterilized water to obtain the seed suspension;

[0041] b. Inoculation: the seed ...

Embodiment 2

[0046] Preparation of Lysobacter enzymogenes 1-T-1-4 wettable powder

[0047] (1) prepare liquid biocontrol agent: with embodiment 1, obtain fermented liquid;

[0048] (2) Add filler: Put the fermentation hydraulic pressure into the storage tank, add diatomite (light calcium carbonate) as filler according to the following calculation formula, and stir for 30 minutes;

[0049] Adding amount of filler (kg)=[number of fermentation broth bacteria (100 million / ml) X tank volume (liter) X yield] / number of finished product bacteria-residue in fermentation broth (kg). Then, it is filtered by plate and frame, beaten, dried, crushed, and made into wettable powder for field use.

[0050] (3) Plate and frame filtration: use 2kg / cm 2 Press the material into the plate frame from the storage tank, filter through No. 7 filter cloth, adjust the pressure at any time, so that the bacteria content in the filtrate is not more than 0.2 billion / ml, and the filter cake and filtrate are obtained;

...

Embodiment 3

[0056] Efficacy Test of Lysobacter enzymogenes 1-T-1-4 on Root Rot of Panax notoginseng

[0057] The reagents and medium used for drug efficacy test are as follows:

[0058] KB medium (peptone 20g, dipotassium hydrogen phosphate 1.5g, magnesium sulfate heptahydrate 1.5g, glycerol 10mL, distilled water 1000mL; pH7.0);

[0059] PDA medium (potato 200g, agar 15g, glucose 15g, water 1000mL);

[0060] NA medium (peptone 5g, beef extract 3g, yeast extract 1g, sucrose 10g, agar 17g, water 1000mL, pH7.0);

[0061] Ethyl acetate, acetone, methanol, petroleum ether, etc. are all analytically pure.

[0062] 1. In vitro antibacterial test of the metabolites of Lysobacter enzymogenes (Lysobacter enzymogenes) 1-T-1-4 on root rot of Panax notoginseng

[0063] 1.1 Pathogens tested

[0064] Including fungi such as Fusarium solani, Fusarium oxysporum, Rhizoctonia solani, Alternaria tenuis and Alternariapanax, above Pathogens were provided by the Plant Pathology Laboratory of Plant Protecti...

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Abstract

The invention particularly relates to Lysobacter enzymogenes 1-T-1-4 and application thereof. The Lysobacter enzymogenes 1-T-1-4 can be prepared into aquae or wettable powder and is used for preventing and treating diseases such as panax notoginseng root rot and amorphophallus konjac soft rot through acting on panax notoginseng or amorphophallus konjac.

Description

technical field [0001] The invention relates to the technical field of biocontrol agents, in particular to an enzyme-producing lysobacterium 1-T-1-4 and its application. Background technique [0002] Sanqi [Panax notoginseng (Burk.) F.H.Chen] belongs to the Araliaceae Ginseng plant. It is a rare and precious Chinese medicinal material unique to my country. In 2014, the planting area of ​​Panax notoginseng in Yunnan Province exceeded 600,000 mu, and its economic value was equivalent to the output value of 20 million mu of corn. The current agricultural output value and the total output value of Panax notoginseng used as raw material in the country exceeded 70 billion yuan. The most important pillar species in the traditional Chinese medicine industry, but the production of Panax notoginseng has serious continuous cropping obstacles. Due to the large-scale single planting in successive years, and the fact that Panax notoginseng is a perennial herb that likes warmth and shade, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N25/02A01P3/00C12R1/01
CPCA01N25/02A01N63/00C12N1/20C12N1/205C12R2001/01A01N2300/00
Inventor 魏兰芳姬广海付丽娜张小芳杨俊
Owner YUNNAN AGRICULTURAL UNIVERSITY
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