Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preservation method of anaerobic ammonium oxidation strain

A technology of anaerobic ammonium oxidation and strain preservation, which is applied in the field of strain preservation, can solve problems such as not involving gas phase conditions, and achieve the effects of complete preservation, small activity loss, good denitrification performance and biological performance

Active Publication Date: 2017-02-22
BEIJING TANSI ENVIRONMENTAL PROTECTION TECHCO
View PDF1 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Existing preservation methods mainly focus on the selection of liquid-phase preservation conditions such as concentrated preservation temperature and the preservation process, such as substrates and protective agents. Although gas-phase conditions are of great significance to the life activities of anaerobic ammonium bacteria, the current method does not involve Optimization of Gas Phase Conditions During Preservation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preservation method of anaerobic ammonium oxidation strain
  • Preservation method of anaerobic ammonium oxidation strain
  • Preservation method of anaerobic ammonium oxidation strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary only for explaining the present invention and should not be construed as limiting the present invention.

[0035]Those skilled in the art will understand that unless otherwise stated, the singular forms "a", "an", "said" and "the" used herein may also include plural forms. It should be further understood that the word "comprising" used in the description of the present invention refers to the presence of said features, integers, steps, operations, elements and / or components, but does not exclude the presence or addition of one or more other features, Integers, steps, operations, elements, components, and / or groups thereof. It will be understood...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

According to the embodiment, the invention provides a preservation method of an anaerobic ammonium oxidation strain. The preservation method comprises the following steps: cultivating the anaerobic ammonium oxidation until it is stable in operation; preparing simulation wastewater; preparing preservation bacterium liquid and transferring the preservation bacterium liquid to a preservation device; air-stripping the preservation bacterium liquid in the preservation device until dissolved oxygen (DO) in the bacterium liquid is lower than 0.1mg / l, regulating pH value of the bacterium liquid to 7.5-7.6, then inflating protective gas to the top of the preservation device and sealing the preservation device; and conducting medium-temperature preservation. According to the method, on the basis of an anaerobic ammonium oxidation bacterium denitrification mechanism, a gaseous phase is transformed into NO gas as the protective gas at a certain concentration in a preserving course, so that the utilization of other denitrifying bacteria on a matrix in the preserving course is effectively inhibited and the matrix is continuously provided to anaerobic ammonium oxidation bacteria; the strain is high in survival rate, low in activity loss and more complete in biological performance preservation; and the method is simple and is easy to implement.

Description

technical field [0001] The invention relates to the technical field of strain preservation, in particular to a method for preserving anammox strains. Background technique [0002] Anaerobic ammonia oxidation is a new type of biological denitrification mode, which can use nitrite as the electron acceptor and ammonia nitrogen as the electron donor to convert the two into nitrogen under anaerobic conditions, so as to realize the denitrification of two kinds of nitrogen pollutants. removed at the same time. Since ammonia nitrogen and nitrite nitrogen can be removed at the same time, there is no need to add organic carbon sources, and it can improve the neutralization of acid production in nitrification reaction and alkali production in denitrification reaction. Its operating cost is nearly 40% lower than that of traditional biological denitrification processes. Therefore, compared with other biological denitrification modes, ANAMMOX has obvious advantages. However, anammox bac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12N1/20C12R1/01
CPCC12N1/04C12N1/20
Inventor 姚宏马金元杨正文
Owner BEIJING TANSI ENVIRONMENTAL PROTECTION TECHCO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products