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Protein separation and purification device and protein separation and purification method

A protein separation and purification device technology, applied in the protein separation and purification device, protein separation and purification field, can solve the problems of high cost, the effect of separation and purification of protein can not meet the ideal requirements, high technical difficulty, short phase separation time, continuous realization The effect of automated and automated production, easy continuous operation

Inactive Publication Date: 2017-02-22
NORTHEAST AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, the current technical problems faced by the separation and purification of proteins are: the technical difficulty and high cost
However, the separation and purification of protein by simple ultrafiltration technology cannot meet the ideal requirements.

Method used

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  • Protein separation and purification device and protein separation and purification method

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Embodiment Construction

[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. The specific embodiments described here are only used to explain the present invention, not to limit the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0022] The present invention provides as figure 1 A kind of protein separation and purification device shown, comprises feed tank 1, the two-phase system 4 that polymer / salt forms and anion exchange resin device 6, and described feed tank 1 is provided with electric stirring device 2, and described electric stirrer The stirring device 2 ...

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Abstract

The invention discloses a protein separation and purification device. A feed pot is communicated with an aqueous two-phase system through a first drainage pipe; a distillation device is communicated with an anion exchange resin device through a second drainage pipe. Compared with the traditional technology, the protein separation and purification device provided by the invention is provided with the feed pot, the aqueous two-phase system comprising polymer / salt, and the anion exchange resin device so as to separately perform the protein separation and purification steps, realizes pollution-free step-by-step and segmented treatment from primary separation and purification of the aqueous two-phase system comprising polymer / salt to secondary separation and purification of the anion exchange resin device through feeding of the feed pot, achieves ideal effect, realizes the separation of the protein through adjusting temperature, pH and the like at an early stage, realizes the continuous and automated production of protein separation and purification, and has high separation and purification efficiency.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a protein separation and purification device. At the same time, the invention also relates to a protein separation and purification method. Background technique [0002] The separation and purification of protein is a method of separating and purifying the desired protein from the mixture with the downstream technology of bioengineering. It is widely used in biochemical research applications and is the core technology of the contemporary bioindustry. Protein separation and purification is a challenging task. Because proteins exist in complex biological systems and are unstable, they are easily deactivated by heat or certain solvents. Therefore, traditional separation techniques such as distillation and solvent extraction are not suitable. It is not suitable for its separation and purification. Chromatography is used for the separation and purification of proteins. Al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/18
CPCC07K1/36C07K1/18C07K1/34
Inventor 姜彬冯志彪刘春红李冬梅
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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