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A kind of dextran cross-linked hemoglobin oxygen carrier and its preparation method and application

The technology of hemoglobin and dextran is applied in the field of blood substitutes of hemoglobin oxygen carrier, which can solve the problems of high oxygen affinity, reduced oxygen carrying-releasing ability, and inability to exert oxygen supply function well, so as to improve physiological functions, increase The effect of improving tetramer stability and oxygen-carrying-releasing capacity

Active Publication Date: 2019-06-04
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, sodium periodate in the reaction solution after oxidizing dextran cannot be completely removed
Since sodium periodate is a strong oxidizing agent, residual sodium periodate and oxidized dextran containing multiple aldehyde groups will oxidize the sulfhydryl group on the side chain of hemoglobin, which will change the microenvironment of heme and cause dextran to cross-link hemoglobin (Dex-Hb) The oxygen affinity is too high, the ability to carry and release oxygen is reduced, and the oxygen supply cannot be well performed, so it cannot be used as a blood substitute

Method used

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  • A kind of dextran cross-linked hemoglobin oxygen carrier and its preparation method and application
  • A kind of dextran cross-linked hemoglobin oxygen carrier and its preparation method and application
  • A kind of dextran cross-linked hemoglobin oxygen carrier and its preparation method and application

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Embodiment

[0042] A series of dextran-crosslinked hemoglobin oxygen carriers were prepared by the above method, only the parameters of the preparation process were slightly adjusted, and the rest of the procedures remained unchanged. The parameters of the preparation process are shown in Table 1.

[0043] Table 1 The parameters of the method for preparing dextran cross-linked hemoglobin oxygen carrier in the present invention

[0044]

[0045]

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Abstract

The invention discloses a dextran cross-linked hemoglobin-based oxygen carrier, a preparation method thereof and an application. The preparation method includes the steps: oxidizing hydroxy on dextran 40 into an aldehyde group by sodium periodate to obtain activated dextran; protecting sulfydryl on hemoglobin by 4-PDS; crosslinking the activated dextran with the bovine hemoglobin closed by the sulfydryl; finally, de-protecting the sulfydryl to obtain the dextran cross-linked hemoglobin-based oxygen carrier. P50 of the dextran cross-linked hemoglobin-based oxygen carrier (Dex-Hb) prepared by the method can be effectively increased, oxygen carrying-releasing capacity is improved, intramolecular crosslinking of hemoglobin tetramer is realized, so that tetramer stability of HBOCs is remarkably enhanced, and physiological functions of the HBOCs are further improved.

Description

technical field [0001] The invention relates to the technical field of blood substitutes of hemoglobin oxygen carriers, in particular to a dextran-crosslinked hemoglobin oxygen carrier and its preparation method and application. Background technique [0002] Hemoglobin oxygen carriers (HBOCs) are a class of substances obtained by encapsulating, modifying or cross-linking hemoglobin by chemical means. The purpose is to increase the molecular weight of free hemoglobin, reduce its nephrotoxicity, and retain its - Ability to release oxygen. However, there are still many scientific problems restricting its development and application in the research of HBOCs to be solved urgently. First of all, hemoglobin is prone to depolymerization after chemical modification or cross-linking, so that tetramers of hemoglobin are easily depolymerized into dimers; second, modification or cross-linking will also change the molecular structure of hemoglobin, making it significantly oxygen-carrying...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/54A61K38/42A61P7/08
CPCA61K9/0026A61K38/42
Inventor 赵莲王瑛周虹胡涛王权章俊赵敬湘
Owner ACADEMY OF MILITARY MEDICAL SCI
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