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Application of VEGF-C in screening of medicine for diagnosing or treating implantation metastasis cancer

A VEGF-C, 1. VEGF-C technology, applied in the medical field, can solve the problem of unclear tumor cell survival spheroid mechanism, and achieve the effect of promoting tumor cell proliferation and improving prognosis.

Inactive Publication Date: 2017-02-22
广州道瑞医药科技有限公司
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  • Claims
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Problems solved by technology

However, how freely detached tumor cells survive in the environment of implantation and metastasis and the mechanism of sphere formation at the initial stage of implantation and metastasis are still unclear.

Method used

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  • Application of VEGF-C in screening of medicine for diagnosing or treating implantation metastasis cancer
  • Application of VEGF-C in screening of medicine for diagnosing or treating implantation metastasis cancer
  • Application of VEGF-C in screening of medicine for diagnosing or treating implantation metastasis cancer

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Experimental program
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Embodiment

[0030] 1. Materials and methods

[0031] 1.1 Study Approval

[0032] All animal experiments were performed after institutional approval by the Yale University Animal Care Committee. Human tumor spheroids from patients with advanced (ie, stage III-IV) ovarian cancer were harvested from ascites fluid provided by informed consent patients, as performed at Yale University School of Medicine, New Haven, Connecticut (HIC protocol #1111003959). Clinical paraffin specimens were used after approval by the Ethical Review Committee of the Cancer Hospital Affiliated to Harbin Medical University, China.

[0033] 1.2 Animal model

[0034] All animal experiments were approved by the Yale University Animal Care Committee. All mice were of the C57BL / 6 series. Lysozyme Cre mice were crossed with tomato (mT / mG) mice to extract cells with the lineage marker lysozyme. Nude mice were purchased from Jackson Laboratory. Put 100ul mouse ovarian cancer ID8 or human ovarian cancer SKOV3 cell...

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Abstract

The invention discloses application of VEGF-C in screening of medicine for diagnosing or treating implantation metastasis cancer, and belongs to the field of medicine. It is proved that TAM relevant spheroids and the ovarian cancer clinical pathology are closely connected, an ovarian epithelial cancer model established in a mouse indicates that TAM promotes spheroid forming and tumor growing in the ovarian cancer implantation metastasis early stage, after EGF excreted by TAM activates EGFR on tumor cells, the EGF up-regulates tumor cells VEGFs-VEGFRs pathway signal transduction in return and then promotes tumor cell proliferation and differentiation, the EGF can up-regulate expression of alphaMbeta2 integral protein on TAM and expression of ICAM-1 on the tumor cells, and the tumor cell-TAM association is promoted. After TAM or medicine retardant VEGF or ICAM-1 in antibodies are removed from the rat, spheroid forming and ovarian cancer progression are hindered. A new mechanism of TAM mediated spheroids is disclosed, and new targeted therapy is provided for ovarian cancer and other distant metastatic cancer.

Description

technical field [0001] The invention relates to the use of VEGF-C in screening drugs for diagnosing or treating implantation metastatic cancer, belonging to the field of medicine. Background technique [0002] Ovarian cancer (OC) is the second most common tumor and one of the leading causes of death in gynecology in the United States. The high mortality rate is mainly due to the difficulty of diagnosing ovarian cancer at an early stage (I / II) until it spreads and progresses to an advanced stage (III / IV). We have reported that the diagnosis rates of OC patients from stage I to IV were 7.19%, 8.63%, 72% and 72.18%, respectively. The prognosis of OC patients is poor, and the average five-year survival rate of OC patients at various stages is 42%. After long-term follow-up observation of patients with stage III and stage IV, the five-year survival rate was found to be less than 10%. However, for patients diagnosed at an early stage (I-II), especially those whose tumors are co...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61P35/04
CPCA61K45/00
Inventor 王敏
Owner 广州道瑞医药科技有限公司
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