Spinibarbus dneticulatus microsatellite family identification method

Abstract

The method discloses a spinibarbus dneticulatus microsatellite family identification method. The method comprises carrying out artificial breeding on spinibarbus dneticulatus parents from the natural population to obtain two families, extracting a genomic DNA, designing and synthesizing a series of microsatellite primer sequences, carrying out PCR amplification on the genomic DNA, screening 9 pairs of the primers with high amplification stability, high polymorphism and high heterozygosity, combining the primers to form three multiplex PCR systems, respectively carrying out PCR amplification on the genomic DNA to obtain a multiplex PCR product, analyzing the multiplex PCR product, and determining the parents in a parent-child relationship with the individual to be detected. The method provides novel technological means for assessment of identification of the spinibarbus dneticulatus family, seed quality management and proliferation and release effects.

Description

technical field [0001] The invention relates to the technical field of fish breeding and molecular markers, in particular to a method for using fluorescent microsatellite markers to identify barb family lines. Background technique [0002] Barbed barb (Spinibarbus denticulatus denticulatus), which belongs to Cyprinformes (Cyprinfomres), Cyprinidae (Cyprindae), Barbinae (Barbinae), spinibarbus (Spinibarbus), commonly known as green bamboo carp, is mainly distributed in the Beijiang River of the Pearl River system in my country. , the middle and upper reaches of the Xijiang River and Dongjiang River, as well as the Changhua River and Nandu River in Hainan Island, are high-quality economic fish in the Pearl River system. Because of its fast growth, large size, miscellaneous food habits, good meat quality, and strong disease resistance, it is a promising breeding variety. [0003] At present, the breeding parents of barb are mainly from wild populations or cultured populations f...

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Problems solved by technology

Due to the differences in environmental factors between different separate culture ponds, etc., this method may lead to deviations in the estimation of genetic parameters related to breeding, which is not conducive to the formulation of breeding plans

[0005] In mixed breeding groups, family information is usually maintained through physical markers such as: fin clipping, branding, listing, fluorescent dyes, etc., but these physical markers also have many problems. The time is not long and it is not easy to mark on the larvae and juveniles, etc.

There is no report on the application of microsatellite markers in barb family identification

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