Monomolecular gene sequencing method and device

Abstract

The invention provides a monomolecular gene sequencing method. The monomolecular gene sequencing method comprises the following steps: growing scattered quantum dot particles on a silicon carbide substrate; guiding tested DNA molecules to enter gaps; acquiring electron distribution of graphene by adopting a single electron electrode array, and transmitting the distribution information to a computer through the fast electronics; reestablishing the time and sinuous track information of the DNA molecules from high-speed signals output by the single electron electrode array; acquiring a system response matrix; and inverting the system response matrix, thus obtaining a vector composed of nucleotide species with sequences. The invention further provides a monomolecular gene sequencing device. The monomolecular gene sequencing device comprises a quantum dot thin film gap module, a DNA molecular movement control module, a single electron read-out module, a system response matrix calculation module and a DNA sequence estimation module. With the adoption of the monomolecular gene sequencing method and the monomolecular gene sequencing device, the sequencing precision of detection can be effectively improved, the sequencing time can be reduced, the biased error caused by gene amplification can be avoided, and the monomolecular gene sequencing method and the monomolecular gene sequencing device are particularly suitable for hand-held terminals and application occasions with high requirement on real-time property.

Description

technical field [0001] The invention relates to the fields of digital signal processing, biological information and biomedical detection, in particular to a single-molecule sequencing method and device. Background technique [0002] Gene sequencing (Gene sequencing, or translation gene sequencing) refers to the analysis of the base sequence of a specific gene fragment, that is, the arrangement of adenine (A), thymine (T), cytosine (C) and guanine (G) Way. The advent of rapid genetic sequencing methods has greatly advanced research and discovery in biology and medicine. [0003] In basic biological research, and in numerous application fields such as diagnostics, biotechnology, forensic biology, biosystematics, knowledge of gene sequences has become indispensable knowledge. The rapid sequencing speed of modern gene sequencing technologies has helped to achieve sequencing of complete DNA (deoxyribonucleic acid, also known as deoxyribonucleic acid) sequences, or multiple type...

AI Technical Summary

Problems solved by technology

[0009] Since the above methods still cannot meet the growing needs of biomedical testing in terms of sequencing time, sequencing cost and sequencing unbiasedness, it is necessary to propose a method with short sequencing time, simple sequencing process, low sequencing cost, high accuracy and no PCR process. Sequencing method without amplification bias

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