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Microbial inoculants used for low temperature fermentation of organic fertilizers, and preparation method and application thereof

A microbial inoculum, low-temperature fermentation technology, applied in the preparation of organic fertilizers, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as affecting the process of manure treatment and stagnation of organic fertilizer production.

Inactive Publication Date: 2017-02-15
BEIJING DAWEIJIA BIOTECH SHARE CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Whether or not heat is produced during the composting process is an important indicator of the success of organic fertilizer fermentation. However, in the cold zone of northern my country, the winter is too cold, causing most microorganisms to enter a dormant state, the fermentation process is terminated, and the production of organic fertilizers has stagnated. Affect the normal manure treatment process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment one, the production of microbial bacterial agent

[0027] Strain screening: All the strains in the microbial agent are isolated from organic fertilizers fermented naturally at - 30 ~ - 10 ℃, and the isolated strains have been subjected to multiple tests of single strains, paired strains and multi-strain compounded organic fertilizers. In the experiment of fermentation effect, the strains that can significantly improve the composting efficiency were screened out, and various safety screening experiments such as bacterial endotoxin, hemolysis, cytotoxicity and biogenic amine were carried out. Finally, the composition of the low-temperature composite bacterial agent was determined as Bacillus subtilis WEI-62, Bacillus licheniformis WEI-67, Bacillus amyloliquefaciens WEI-79, Enterococcus faecium WEI-89 and Candida metagenes WEI-107.

[0028] Strain cultivation: Bacillus subtilis WEI-62, Bacillus licheniformis WEI-67, Bacillus amyloliquefaciens WEI-79, Enterococcu...

Embodiment 2 1

[0035] The preparation of embodiment two, 1# organic fertilizer

[0036] Under the temperature condition of - 30 ~ - 10 ℃, take 1000 kg of pig manure mixed with 200 kg of chopped straw, add 12 L of the microbial agent of the present invention at the same time, mix evenly with a forklift, and adjust the water content with biogas fermentation liquid is 63%.

[0037] Experimental group: After the start of fermentation, the high temperature reached above 60°C on the second day, and the temperature remained at around 70°C until the eighth day, and the temperature dropped to 50°C on the ninth day, and the temperature rose to 70°C soon after turning over. On the 16th day, the temperature dropped to 40°C. After the second turning, the temperature rose again and remained between 50-60°C. On the 20th day, the temperature dropped below 20°C. The fermentation ended and the composting took about 10 days. Always keep the moisture content between 55-65%.

[0038] Control group (no bacteria...

Embodiment 3 2

[0039] The preparation of embodiment three, 2# organic fertilizer

[0040] Under the temperature condition of - 30 ~ - 10 ℃, 1000 kg of pig manure was mixed with 200 kg of rice husk powder, and 12 L of the microbial agent of the present invention was added at the same time, mixed evenly with a forklift, and the water content was adjusted with biogas fermentation liquid to 60%.

[0041] Experimental group: After the start of fermentation, the temperature reached above 60°C on the second day, and the temperature dropped to 50°C on the 11th day. After turning over the compost, the temperature quickly rose to 70°C. After the second compost turning, the temperature is kept at about 60°C, and the temperature drops to 20°C on the 23rd day. After the fermentation is over, the compost is matured for about 10 days, and the water content is always kept between 55-65% during the process.

[0042] Control group (no bacterial agent added): the temperature was at 30°C on the first day, and ...

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Abstract

The purpose of the invention is to provide a microbial inoculant used for low temperature fermentation of organic fertilizers. The microbial inoculant comprises Bacillus subtilis WEI-62, Bacillus licheniformis WEI-67, Bacillus amyloliquefaciens WEI-79, Enterococcus faecium WEI-89 and Candida utilis WEI-107, wherein the total quantity of Bacillus subtilis is 1.26 * 10<9> CFU / mL, the total quantity of Bacillus spp is 2.93 * 10<8> CFU / mL, and the total quantity of yeast is 5.74 * 10<8> CFU / mL. Compost adopting livestock manure ad a main raw material is inoculated with 1-5% of the microbial inoculants at -30 ~ -10 DEG C, and heating, fermentation, cooling and decomposition processes of the compost are successfully realized a period of time later in order to prepare the high-quality organic fertilizer. The composite microbial inoculant has the characteristics of fast fermentation heating, long high temperature keeping time and secondary heating under cold region extreme cold winter conditions, and is mainly suitable for large-scale livestock culture farms for processing manure under extreme cold conditions in the north region to produce the organic fertilizer.

Description

[0001] Technical field: [0002] The present invention relates to a microbial bacterial agent, in particular to a microbial bacterial agent for low-temperature fermented organic fertilizer and its preparation method and application [0003] Background technique: [0004] In China, the treatment of aquaculture manure has always been a pain point in the aquaculture industry, restricting the sustainable development of the entire industry. With the strengthening of environmental protection in the past two years, the problem of manure treatment has risen to determine whether the aquaculture enterprise will be shut down It is urgent to solve the problem of manure treatment. The use of microbial fermentation technology to produce organic fertilizers can not only solve the environmental pollution problem of manure, but also provide green and high-quality fertilizers for the planting industry, which can be said to serve multiple purposes. [0005] The use of microbial fermentation tech...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C05F15/00C05F11/08C05F17/00C12R1/125C12R1/10C12R1/07C12R1/72C12R1/01
CPCC05F3/00C05F11/08C12N1/16C12N1/20C05F11/00C05F17/00Y02E50/30Y02W30/40
Inventor 乔琳高长斌姚宏明刘蕊冯媛媛
Owner BEIJING DAWEIJIA BIOTECH SHARE CO LTD
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