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Method for promoting rapid accumulation of astaxanthin in haematococcus pluvialis

A technology of Haematococcus pluvialis and astaxanthin is applied in the field of promoting the rapid accumulation of astaxanthin in Haematococcus pluvialis, which can solve the stagnation or even reversal of the accumulation process of astaxanthin, the high price of astaxanthin and the economic loss. and other issues, to achieve the effect of important economic value and operability, low price and resource saving

Pending Publication Date: 2017-02-15
NINGBO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Haematococcus pluvialis cultivation methods mainly include closed photobioreactor and open raceway pond, among which the cultivation technology of photobioreactor is relatively mature, but the high price of astaxanthin caused by the high-cost reactor is a constraint. The bottleneck of the development of the Haematococcus pluvialis industry; the cultivation of Haematococcus pluvialis in the track pool is cheap, but it is easy to cause pollution, and the use of stress methods such as sunlight to promote the accumulation of astaxanthin in cells is severely restricted by weather conditions, cloudy or sunny When it is weak, it will cause the process of astaxanthin accumulation to stagnate or even reverse, resulting in greater economic losses

Method used

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  • Method for promoting rapid accumulation of astaxanthin in haematococcus pluvialis
  • Method for promoting rapid accumulation of astaxanthin in haematococcus pluvialis
  • Method for promoting rapid accumulation of astaxanthin in haematococcus pluvialis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] First carry out the cultivation of Haematococcus pluvialis in the 250L runway pond, after the algal cell growth to the logarithmic phase, measure the cell concentration (OD680=0.35), the algae liquid is filtered to the microporous membrane that the aperture is 20 microns ( The filtrate is continuously cultivated in the raceway pool). If the solar radiation is not enough, artificial light can be used to induce astaxanthin; use an inducing moisturizer (pH 1.0, salicylic acid concentration 25 μg / mL) to keep the filter membrane in a wet state (relative humidity 90%). After 7-10 days, after the Haematococcus pluvialis turns red, the algae body can be collected and used as an experimental group to measure the astaxanthin content in the Haematococcus pluvialls.

[0024] At the same time, under the same breeding conditions, that is, under the condition of insufficient solar radiation, the experiment of the control group was carried out. Continue the original conditioned cultur...

Embodiment 2

[0029] It is basically the same as Example 1, except that the experimental material used is Haematococcus pluvialis cultivated in a 20L airlift photobioreactor, and astaxanthin is accumulated through the same technical method. The finally obtained data are shown in Table 2, and all data are the average value of three determinations. The results show that the Haematococcus pluvialis cultivated in the photobioreactor utilizes the method of the present invention (filtering+artificial light+spraying to induce moisturizing agent) to accumulate astaxanthin, and the highest content is 1.59%. ) were significantly different (p0.05).

[0030] Table 2: Changes of astaxanthin content (%) in Haematococcus pluvialis under different culture modes

[0031]

Embodiment 3

[0033] It is basically the same as Example 1, except that the microporous membrane used is 10 micron pore size, and the accumulation of astaxanthin is carried out through the same technical method. The final data obtained are shown in Table 3, and all data are the average value of three determinations. The results showed that the average value of astaxanthin accumulated by Haematococcus pluvialis was 1.78%, which was not significantly different from that of the filter membrane with a pore size of 20 microns (p>0.05).

[0034] Table 3 The influence of different filter conditions on the accumulation of astaxanthin in Haematococcus pluvialis (%)

[0035]

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Abstract

The invention provides a method for promoting rapid accumulation of astaxanthin in haematococcus pluvialis. The method comprises the following steps: collecting haematococcus pluvialis cells in a post-log phase and / or a platform stage; keeping humidity of the haematococcus pluvialis cells at 70-97%; and accumulating the astaxanthin under the condition of illumination. By the method, rapid accumulation of the astaxanthin is promoted, and the shortcomings that a traditional raceway pond production mode is restricted by solar radiation and weather conditions, the accumulation period of the astaxanthin is long and the like can be overcome. By the technical method, the astaxanthin in the haematococcus pluvialis not only can be accumulated in the open air, but also can be accumulated under an artificial light source, and restriction of the weather conditions is solved.

Description

technical field [0001] The invention belongs to the technical field of astaxanthin preparation, in particular to a method for promoting rapid accumulation of astaxanthin in Haematococcus pluvialis. Background technique [0002] Astaxanthin is a carotenoid substance with super antioxidant activity. It is mainly used in health care, medical treatment, food, aquaculture bait, etc., and has a very broad market prospect. The global astaxanthin market was reported to be worth approximately US$447 million in 2014 (Algae Industry Magazine.com: 2016-05-25). Haematococcus pluvialis is currently the best raw material for producing natural astaxanthin. The green vegetative cell stage is mainly the biomass accumulation stage of algae cells, and the red sclerenchyma cell stage is the astaxanthin accumulation stage. Therefore, the key to the development of Haematococcus pluvialis industry is to increase the growth rate of algae cells and promote the accumulation of astaxanthin. [0003] ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P23/00C12R1/89
CPCC12N1/12C12P23/00
Inventor 徐年军李艳国孙雪张琳杨柳
Owner NINGBO UNIV
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