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a cell culture chamber

A cell culture and box technology, applied in tissue cell/virus culture devices, biomass post-processing, specific-purpose bioreactors/fermenters, etc., can solve structural instability, resonance vibration signal measurement value error, Problems such as placing it for a long time

Active Publication Date: 2018-10-30
SOUTHWEST JIAOTONG UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a cell culture box, which can effectively solve the problem of placing the culture body for a long time under the condition of leaving the culture environment
At the same time, it can effectively solve the problem that the internal structure of the incubator is unstable, which leads to the resonance of the built-in vibration device, and the problem that the error of the measured value of the generated vibration signal is higher than the allowable error of the experiment.

Method used

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  • a cell culture chamber

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Embodiment 1

[0017] A cell culture box, comprising an upper box body, a lower box body 4 and a controller, the upper box body is a cell culture chamber 9, the lower box body 4 is provided with an excitation device 6 with an elastic support base 5; the upper box body There is a through hole in the middle of the bottom plate 7, and a vertical bar is provided at the lower part of the vibrating table 8 at the bottom of the cell culture chamber 9, and the vertical bar passes through the through hole of the bottom plate 7 and is fixed with the vibration excitation device 6 in the lower box; the vibration excitation power amplifier The output of 1 is connected to the vibration excitation device 6, the vibration signal of the vibration excitation device 6 is connected to the vibration signal measuring instrument 3 through the vibration sensor, and the output terminal of the vibration signal adjustment controller 2 is connected to the vibration excitation power amplifier 1 line.

[0018] The vibrati...

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Abstract

The invention provides a cell culture box and relates to the technical field of biological experiment equipment. It solves the problem that the culture body is placed for a long time under the condition of leaving the culture environment. The upper box body is a cell culture chamber 9, and the lower box body 4 is provided with an excitation device 6 with an elastic support base 5; the bottom plate of the upper box body There is a through hole in the middle of 7, and a vertical rod is provided at the lower part of the vibrating table 8 at the bottom of the cell culture chamber 9, and the vertical rod passes through the through hole of the bottom plate 7 and is fixed to the vibration excitation device 6 in the lower box; the vibration excitation power amplifier 1 The output of the vibration excitation device 6 is connected, the vibration signal of the vibration excitation device 6 is connected with the vibration signal measuring instrument 3 through the vibration sensor, and the output terminal of the vibration signal adjustment controller 2 is connected with the vibration excitation power amplifier 1 line. The vibrating table 8 is made of aluminum alloy plate, and the vibrating table 8 is provided with even-numbered equidistant screw holes. It is mainly used for cell culture to provide micro-vibration stress stimulation in the experimental environment.

Description

technical field [0001] The invention relates to the technical field of biological experiment equipment. Background technique [0002] Mechanical stress in vivo is a key inducer that plays a crucial role in bone growth and repair. Studies have shown that, as a stress stimulus, vibration has a good effect on promoting bone formation and bone reconstruction. Vibration stress is beneficial to bone maturation and remodeling, and vibration intensity, vibration frequency, and vibration amplitude affect the osteogenesis effect of vibration. In general, the mechanical environment with amplitude ≤ 50 μm, intensity < 1×g, and frequency range of 1-100HZ is considered to be a suitable vibration stress stimulation environment. The low amplitude, low intensity , Low-frequency vibration is also called micro-vibration. So far, the specific biological mechanism of micro-vibration is not very clear. The cellular level experiment of the micro-vibration biological effect is an important a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/36
CPCC12M27/16C12M35/04C12M41/00
Inventor 智伟翁杰匙峰何旭周腾李嘉段可汪建新屈树新
Owner SOUTHWEST JIAOTONG UNIV
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