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Tissue culture method of Aralia Cordata Thunb seedlings

A tissue culture, soil angelica technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of high cost and long production cycle, and achieve the effects of low cost, low production cost and easy operation.

Active Publication Date: 2017-02-15
资源县银竹老山中草药种植专业合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for using edible soil Angelica sinensis leaves as explants to tissue culture its seedlings, so as to overcome the long planting production cycle and high cost of the existing soil Angelica sinensis.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for tissue culture of edible soil angelica seedlings, comprising the steps of:

[0019] (1) Explant treatment: Take the leaves of Angelica sinensis as explants, wash off the dust on the surface with water, soak in 70% alcohol for 20-30s, rinse with sterile water 2-3 times, and then use 0.1% mercury chloride The solution was soaked and disinfected for 7-10 minutes, then rinsed with sterile water for 3-5 times, and finally dried the surface moisture of the explants with sterile filter paper;

[0020] (2) Primary culture of explants: Cut the treated explants into small pieces of 1.0 cm×1.0 cm with a scalpel and inoculate them in MS +TDZ0.1 mg / L+6-BA0.1 mg / L+ NAA0.5mg / L + sucrose 30g / L + agar 6.5g / L, cultured in medium with pH 5.8, culture temperature 25±2℃, light time 12h·d-1, light intensity 2000~3000lx, cut edge at 21d Start to induce clusters of small buds at the place, the induction rate is 100%, and the proliferation rate is counted after 50 days of cultivat...

Embodiment 2

[0024] A tissue culture method for edible soil angelica seedlings, including the method steps of embodiment 1, the difference is: the explants of the primary culture are inoculated in MS+TDZ0.1 mg / L+6-BA0.5mg / L+ NAA0.5mg / L + sucrose 30g / L + agar 6.5g / L medium. On the 13th day, a large number of small buds germinated on the edge of the leaves and on the surface of the explants, and the buds were scattered on the explants. The buds were strong and light green, and the induction rate was 66.7%. After culturing for 50 days, the proliferation rate was counted, and the number of seedlings induced by a single leaf was 35-50.

Embodiment 3

[0026] A tissue culture method for edible soil angelica seedlings, comprising the method steps of Example 1, the difference is that the explants of the primary culture are inoculated in MS+6-BA0.1mg / L+NAA0.05mg / L+sucrose 30g / L+ agar 6.5g / L medium. On the 19th day, small buds began to be induced at the edge of the incision, and the induction rate was 100%. After culturing for 50 days, the proliferation rate was counted, and the number of seedlings induced by a single leaf was 40-60.

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Abstract

The invention discloses a tissue culture method of Aralia Cordata Thunb seedlings. The method comprises the following steps: inducing calluses from leaves of Aralia Cordata Thunb, allowing the calluses to form adventitious embryos, carrying out subculture multiplication on the adventitious embryos, carrying out cycle culture separation to obtain single adventitious embryos, and culturing the single adventitious embryos on a solid medium to form the seedlings. The tissue culture seedlings cultured by the selected medium with reasonable formula have high rooting rate and good stability. The method is easy to operate, can be carried out in four seasons without restriction of extraneous conditions, saves the occupied area of growth of the seedlings, reduces the production cost, has no pollution to environment, and realizes batch production. The method for producing Aralia Cordata Thunb also has the advantages of large output, low cost, short period, high market competitiveness, convenience in material drawing, no influences on the quantity of Aralia Cordata Thunb wild populations, simplicity in operation, low production cost, good stability, high efficiency, great shortening of the seedling growth cycle, and provision of technical guaranteeing for protection, development and utilization of Aralia Cordata Thunb.

Description

technical field [0001] The invention relates to tissue culture, in particular to a tissue culture method for edible soil angelica seedlings. Background technique [0002] Edible soil Angelica is a perennial herb. It is a traditional Chinese medicine with both medicine and food. Its tender stems and leaves can be eaten as vegetables, fried, cold or pickled; Invigorate blood circulation, relieve heat and relieve pain, diuretic and detumescence, dispel wind and invigorate deficiency, and can be used for rheumatic arthralgia, soreness of waist and knees, headache, dizziness, edema and other diseases. At present, the aboveground part of Angelica sinensis is one of the wild green vegetables that are in short supply in domestic and international markets, and has great economic value. However, its wild resources have been plundered, and its growth A large area has been greatly reduced. [0003] Under normal circumstances, the planting cycle of Angelica sinensis is generally 2 year...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/008
Inventor 柳福权
Owner 资源县银竹老山中草药种植专业合作社
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