Induction Method of Loose Embryogenic Callus of Potato

Abstract

The invention discloses a method for inducing embryogenic callus of potato loose type. The main content is that potato tubers are used as explants to induce soft potato callus on a low osmotic pressure MS medium containing 2,4‑D. callus; then transfer the soft callus to normal osmotic pressure containing mitogen and 2,4‑D MS medium to induce loose callus, after multiple subcultures and selective inoculation plant, and finally obtain loose potato callus; transfer the obtained potato loose callus to a medium containing a certain concentration of mitogens and 2,4‑D, undergo multiple subcultures, and select Appropriate culture temperature, combined with selective inoculation of callus during subculture, finally obtained potato callus with loose structure and embryogenic characteristics. The invention establishes a set of potato loose embryogenic callus induction system, which lays the foundation for the virus-free cultivation of potatoes.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and relates to an induction method for obtaining potato loose embryogenic callus by tissue culture technology. Background technique [0002] Potatoes are commonly known as potatoes in the vast crop seed areas of our country. Potatoes are edible, "clothable vegetables", "raised", and "processable" food crops. . In recent years, through extensive research on potato cultivation and breeding in vast areas of our country, many breakthroughs have been made, especially outstanding achievements have been made in potato tissue culture. [0003] In potato production, tubers are often used for planting. After the promotion of new varieties, as the planting years increase, the virus also accumulates in the tubers with the increase in the asexual generation of seed potatoes, resulting in frequent plant dwarfing, clustering, and stalks in the field. Thin and weak, reduced branches; shrunken, curled, a...

AI Technical Summary

Problems solved by technology

[0005] At present, potato virus-free culture mainly relies on shoot tip virus-free technology, and there are very few reports on the use of loose embryogenic callus for tissue virus-free. Difficult obstacles in loose embryogenic callus

There have been some reports on the use of callus culture for plant tissue detoxification, and it has been successful in some plant studies, but it has not been reported on potatoes, so it can be preliminarily judged that it may be due to the difficulty of potato embryogenic callus Induced reason