Induction method for loose embryonic callus of potatoes

Abstract

The invention discloses an induction method for the loose embryonic callus of potatoes. The induction method has the main contents that a potato tuber is taken as an explant, the loose callus of the potato is induced on a hyposmolality MS culture medium which contains 2,4-D; the loose callus is transferred to an MS culture medium which has normal osmotic pressure and contains mitogen and 2,4-D to carry out the induction of the loose callus, multi-time subculture is carried out, the explant is selectively inoculated, and finally, the loose callus of the potatoes is obtained; the obtained loose callus of the potatoes is transplanted to a culture medium which contains the mitogen of a certain concentration and 2,4-D, multi-time subculture is carried out, a proper culture temperature is selected, and selective inoculation for the callus during the subculture is combined to finally obtain the potato callus with a loose structure and embryogenic characteristics. The invention establishes one set of induction system of the loose embryonic callus of the potatoes, and a foundation is laid for the devirusing culture of the potatoes.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and relates to an induction method for obtaining potato loose embryogenic callus by tissue culture technology. Background technique [0002] Potatoes are commonly known as potatoes in the vast crop seed areas of our country. Potatoes are edible, "clothable vegetables", "raised", and "processable" food crops. . In recent years, through extensive research on potato cultivation and breeding in vast areas of our country, many breakthroughs have been made, especially outstanding achievements have been made in potato tissue culture. [0003] In potato production, tubers are often used for planting. After the promotion of new varieties, as the planting years increase, the virus also accumulates in the tubers with the increase in the asexual generation of seed potatoes, resulting in frequent plant dwarfing, clustering, and stalks in the field. Thin and weak, reduced branches; shrunken, curled, a...

AI Technical Summary

Problems solved by technology

[0005] At present, potato virus-free culture mainly relies on shoot tip virus-free technology, and there are very few reports on the use of loose embryogenic callus for tissue virus-free. Difficult obstacles in loose embryogenic callus

There have been some reports on the use of callus culture for plant tissue detoxification, and it has been successful in some plant studies, but it has not been reported on potatoes, so it can be preliminarily judged that it may be due to the difficulty of potato embryogenic callus Induced reason