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Fast culture method of rhodiola kirilowii(regel)maxim. seedlings

A technology of Rhodiola serrata and a cultivation method, which is applied in the field of rapid cultivation of Rhodiola serrata seedlings

Active Publication Date: 2017-02-15
SICHUAN XIELI PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent uses the cotyledon petiole of Rhodiola angustifolia as the explant, and obtains the regenerated plant of Rhodiola angustifolia by inducing the embryoid body. At present, there is no report on this method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The rapid cultivation method of Rhodiola angustifolia seedling that the present embodiment provides comprises the steps:

[0020] (1) Obtaining of explants:

[0021] a. Select fully mature Rhodiola angustifolia seeds, put them into 0.1% mercuric chloride solution at a volume ratio of 1:20 to sterilize them for 4 minutes, and then wash them with sterile water for 5 minutes. Then insert MS+yeast extract 80mg·L -1 In the culture medium, under the conditions of 12 hours of light per day, 1000 lx of light intensity and 12 hours of cultivation at 7°C and 22°C every day, the germination and growth of the seeds were carried out. After 5 days of cultivation statistics, the infection rate of Rhodiola angustifolia seeds was 0%, the germination rate is 100%;

[0022] b. Take the germinated Rhodiola angustifolia seeds and transfer them to MS+ Chuanqiao No. 1 seeds 80g L -1 In the culture medium, after cultivating for 20 days under the condition that the culture temperature is 20°...

Embodiment 2

[0028] The rapid cultivation method of Rhodiola angustifolia seedling that the present embodiment provides comprises the steps:

[0029] (1) Obtaining of explants

[0030] a. Select the fully mature Rhodiola angustifolia seeds, put them into 0.1% mercuric chloride solution at a volume ratio of 1:20 to sterilize them for 3 minutes, and then wash them with sterile water for 3 minutes. Then insert MS+yeast extract 50mg·L -1 In the culture medium, under the conditions of 10 hours of light per day, 700 lx of light intensity and 12 hours of cultivation at 5°C and 20°C every day, the germination and growth of the seeds were carried out. After 5 days of cultivation statistics, the infection rate of Rhodiola angustifolia seeds was 5.56%, the germination rate is 95.15%;

[0031] b. Take the germinated Rhodiola angustifolia seeds and transfer them to MS+ Chuanqiao No. 1 seeds 50g L -1 In the culture medium, after culturing for 20 days under the conditions of a culture temperature of 1...

Embodiment 3

[0037] The rapid cultivation method of Rhodiola angustifolia seedling that the present embodiment provides comprises the steps:

[0038] (1) Obtaining of explants

[0039] a. Select fully mature Rhodiola angustifolia seeds, put them into 0.1% mercuric chloride solution at a volume ratio of 1:20 to sterilize them for 7 minutes, and then wash them with sterile water for 6 minutes. Then insert MS+yeast extract 100mg·L -1 In the culture medium, under the conditions of 14 hours of light per day, light intensity of 1200lx and 12 hours of cultivation at 8°C and 25°C every day, the germination and growth of seeds were carried out, and the statistics of cultivation for 5 days showed that the infection rate of Rhodiola angustifolia seeds was 0%, the germination rate was 81.24%;

[0040] b. Take the germinated Rhodiola angustifolia seeds and transfer them to MS+ Chuanqiao No. 1 seeds 100g L -1 In the culture medium, after culturing for 20 days under the conditions of a culture tempera...

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PUM

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Abstract

The invention discloses a fast culture method of rhodiola kirilowii(regel)maxim. seedlings; the method takes rhodiola kirilowii(regel)maxim. cotyledonary petioles as explants, through induction culture of explant embryogenic cells and embryoids, the rhodiola kirilowii(regel)maxim. high-quality seedlings are successfully cultured, and a new way is provided for massive fast culture of the rhodiola kirilowii(regel)maxim. high-quality seedlings.

Description

technical field [0001] The invention relates to a method for quickly cultivating Rhodiola angustifolia seedlings, in particular to a method for realizing rapid breeding of Rhodiola angustifolia plants by inducing embryoid bodies of Rhodiola angustifolia. Background technique [0002] Rhodiola kirilowii (Regel) Maxim. is Rhodiola kirilowii (Regel) Maxim. It is a perennial herb of the Sedum family Rosaceae. Distributed in my country's Tibet, Qinghai, Sichuan, Yunnan and other places in the alpine areas with an altitude of 2000-5600m, it is a famous alpine Chinese and Tibetan medicinal material in my country. Rhodiola angustifolia has the functions of clearing away heat, reducing fever, detoxifying and preventing plague, and can treat pneumonia, fever, diarrhea and swelling of limbs. Modern pharmacological studies have shown that Rhodiola has the effects of reducing heat and detoxification, anti-fatigue, anti-hypoxia, and anti-aging. Rhodiola angustifolia is a dioecious plant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王跃华刘洪明昝加志赵江林钟灵允彭世明何新友何诗红刘燕刘鑫罗倩陈芳何美翠陈沙彭梅
Owner SICHUAN XIELI PHARM CO LTD
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