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Serum separation method and equipment thereof

A serum separation and equipment technology, which is applied in the preparation and sampling of analytical materials and samples for testing, can solve the problems of complex mechanical structure, loss of blood samples, pollution, etc. Effect

Active Publication Date: 2017-02-08
武汉雁达生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, blood testing is the most widely used sample testing in biomedicine, clinical diagnosis, health and quarantine diagnosis, but the presence of red blood cells has a great interference on the spectral analysis in blood testing, so how to quickly and easily separate serum from whole blood It has always been a hot and difficult issue in blood testing research
[0003] At present, most hospitals and laboratories still use large centrifuges to separate serum and blood cells. This method requires blood samples in milliliters to be drawn from patients, but the samples actually used for testing are only tens or even several microliters, resulting in A large amount of waste; and this kind of centrifuge equipment is large in size and complex in mechanical structure. The serum separation process and detection are carried out separately and independently, which is easy to cause the loss and contamination of blood samples. It is not suitable for the integration of clinical diagnosis and micro-analysis systems, and cannot meet medical The need for the development of micro-analysis system in diagnosis, so it is necessary to develop a new micro-separation technology

Method used

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  • Serum separation method and equipment thereof
  • Serum separation method and equipment thereof
  • Serum separation method and equipment thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] S1. Extract blood samples: extract the blood samples to be tested from the human body and put them into multiple blood collection tubes, and block all the ports of the blood collection tubes containing the blood samples;

[0030] S2. Place the blood collection tubes: place the blood collection tubes completed in S1 on the blood vessel tray, and place the blood collection tubes on the blood vessel tray from inside to outside in sequence;

[0031] S3. Stand still: the blood vessel tray filled with blood collection tubes is left to stand for 5 minutes;

[0032] S4. Centrifugation: put the static blood collection tube into the centrifuge, and centrifuge for 1 minute at a centrifugal rate of 2000 rpm;

[0033] S5. Stand still in a water bath: put the blood collection tube in a constant temperature water bath box, and the temperature of the constant temperature water bath box is 37 degrees, and let it stand for 4 minutes;

[0034] S6. Stripping fibrin: open the blood collect...

Embodiment 2

[0039] S1. Extract blood samples: extract the blood samples to be tested from the human body and put them into multiple blood collection tubes, and block all the ports of the blood collection tubes containing the blood samples;

[0040] S2. Place the blood collection tubes: place the blood collection tubes completed in S1 on the blood vessel tray, and place the blood collection tubes on the blood vessel tray from inside to outside in sequence;

[0041] S3. Stand still: the blood vessel tray filled with blood collection tubes is left to stand for 8 minutes;

[0042] S4. Centrifugation: put the static blood collection tube into a centrifuge, and centrifuge for 2 minutes at a centrifugal rate of 2300 rpm;

[0043] S5. Stand still in a water bath: put the blood collection tube in a constant temperature water bath box, and the temperature of the constant temperature water bath box is 37 degrees, and let it stand for 4 minutes;

[0044] S6. Stripping fibrin: open the blood collecti...

Embodiment 3

[0049] S1. Extract blood samples: extract the blood samples to be tested from the human body and put them into multiple blood collection tubes, and block all the ports of the blood collection tubes containing the blood samples;

[0050] S2. Place the blood collection tubes: place the blood collection tubes completed in S1 on the blood vessel tray, and place the blood collection tubes on the blood vessel tray from inside to outside in sequence;

[0051] S3. Stand still: the blood vessel tray filled with blood collection tubes is left to stand for 10 minutes;

[0052] S4. Centrifugation: put the static blood collection tube into a centrifuge, and centrifuge for 2 minutes at a centrifugal rate of 2500 rpm;

[0053] S5. Stand still in a water bath: place the blood collection tube in a constant temperature water bath box, and the temperature of the constant temperature water bath box is 37 degrees, and let it stand for 5 minutes;

[0054] S6. Stripping fibrin: open the blood colle...

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Abstract

The invention relates to the technical field of serum separating, and provides a serum separating method. The method comprises the following steps: S1) extracting a blood sample; S2) putting the blood collection tubes; S3) standing; S4) centrifuging; S5) performing water-bath standing; S6) stripping fibrin; and S7) performing centrifugation. The invention provides serum separating equipment, which comprises an L-shaped work bench, a centrifuge and a constant temperature water-bath case, the centrifuge is arranged at a middle part of the L-shaped work bench, a hollow column is arranged at the middle part of an inner core of the constant temperature water-bath case, and a hollow support bar is fixedly connected with a rotating cylinder in the centrifuge. The serum separation method is simple, no chemical reagent is added in the serum separation method, the original component of a blood sample is not changed, no interference and influence can be generated on the blood sample determination, the equipment is the integrated equipment through the constant temperature water-bath case and the centrifuge, a series of serum separating flow can be completed, and the separation for serum is convenient.

Description

technical field [0001] The invention relates to the technical field of serum separation, in particular to a serum separation method and equipment. Background technique [0002] Biochemical medical testing based on human body fluids such as blood, urine and saliva can provide important information that reflects people's health status. At present, blood testing is the most widely used sample testing in biomedicine, clinical diagnosis, health and quarantine diagnosis, but the presence of red blood cells has a great interference on the spectral analysis in blood testing, so how to quickly and easily separate serum from whole blood It has always been a hot and difficult issue in blood testing research. [0003] At present, most hospitals and laboratories still use large centrifuges to separate serum and blood cells. This method requires blood samples in milliliters to be drawn from patients, but the samples actually used for testing are only tens or even several microliters, res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28G01N1/2813G01N2001/2846
Inventor 谭现花
Owner 武汉雁达生物技术有限公司
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