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Kit for measuring total cholesterol and method

A technology of total cholesterol and kit, applied in the field of medical testing, can solve the problems of masking, affecting the prognosis of patients, delaying the disease, etc.

Active Publication Date: 2017-02-01
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The interference of calcium dobesilate and phenetamine on the enzymatic measurement of total cholesterol is a major inconvenience to patients who are administered these two drugs
For example, for diabetic patients, they are often accompanied by coronary heart disease, vascular arteriosclerosis, etc., and the interference of the administered calcium dobesilate on the enzymatic detection of total cholesterol may cause the total cholesterol level of diabetic patients to be incorrectly evaluated, covering up and delay illness
For patients undergoing surgery, the interference of the administered ethanol on the enzymatic detection of total cholesterol may make it impossible to correctly assess their total cholesterol levels, resulting in postoperative complications and affecting the prognosis of patients
At present, the enzymatic detection kits sold on the market generally have the defect that they cannot effectively resist the interference of calcium dobesilate and phensulfonate

Method used

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  • Kit for measuring total cholesterol and method
  • Kit for measuring total cholesterol and method
  • Kit for measuring total cholesterol and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] Embodiment 1: the preparation of cholesterol assay kit

[0093] According to the components and concentrations shown in Table 6 and Table 7 below, the components were mixed to prepare reagent sets 1 and 2, respectively.

[0094] Table 6: R1 formulation

[0095]

[0096] Table 7: R2 formulation

[0097]

Embodiment 2

[0098] Embodiment 2: the preparation of cholesterol assay kit

[0099] According to the components and concentrations shown in Table 8 and Table 9 below, the components were mixed to prepare reagent sets 1 and 2, respectively.

[0100] Table 8: R1 formulation

[0101]

[0102] Table 9: R2 formulation

[0103]

Embodiment 3

[0104] Embodiment 3: the preparation of cholesterol assay kit

[0105] According to the components and concentrations shown in Table 10 below and Table 11 below, the components were mixed to prepare reagent sets 1 and 2, respectively.

[0106] Table 10: R1 formulation

[0107]

[0108] Table 11: R2 formulation

[0109]

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PUM

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Abstract

The invention relates to a kit for measuring total cholesterol and a method. The kit comprises a reagent group 1 and a reagent group 2, wherein the reagent group 1 comprises chromogen and cholesterol esterase; the reagent group 2 comprises 4-aminoantipyrine, peroxidase and cholesterol oxidase; the chromogen comprises N-ethyl-N-(2-hydroxyl-3-sulfopropyl)-3, 5-dimethoxyaniline. The kit and the method can reduce interference caused by calcium dobesilate and / or etamsylate in samples during total cholesterol detection.

Description

technical field [0001] The invention relates to the technical field of medical testing. In particular, the present invention relates to a kit and method for detecting total cholesterol. Background technique [0002] Cholesterol, also known as cholesterol, is a derivative of cyclopentane polyhydrophenanthrene. Cholesterol exists in lipoproteins in the blood, and its existing forms include high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and very low-density lipoprotein cholesterol. Cholesterol is widely present in animals, especially in brain and nerve tissue, and is also high in kidney, spleen, skin, liver and bile. Cholesterol is one of the indispensable important substances in animal tissue cells. It not only participates in the formation of cell membranes, but also is an important raw material for the synthesis of adrenal cortex hormones, bile acids, vitamin D and other physiologically active substances, and is also the main component of cell m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/60C12Q1/44
CPCC12Q1/44C12Q1/60C12Q2334/00
Inventor 吴昌英罗岚
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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