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Application of miR-21 in preparing EnMSCs (endometrium derived mesenchymal stem cells) paracrine effect regulator

A mir-21, 1.mir-21 technology, applied in the direction of analytical materials, biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as no clear conclusions, and achieve the effect of inhibiting and anti-apoptosis

Inactive Publication Date: 2017-02-01
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, there have been reports about the paracrine effect of EnMSC to improve the therapeutic effect of myocardial infarction, but there has been no clear conclusion on how to regulate the paracrine process of EnMSC.

Method used

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  • Application of miR-21 in preparing EnMSCs (endometrium derived mesenchymal stem cells) paracrine effect regulator
  • Application of miR-21 in preparing EnMSCs (endometrium derived mesenchymal stem cells) paracrine effect regulator
  • Application of miR-21 in preparing EnMSCs (endometrium derived mesenchymal stem cells) paracrine effect regulator

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Embodiment 1

[0026] 1. Purchase EnMSCs from Hangzhou Yiwensai Biotechnology Co., Ltd., culture the primary and passage cells in low-sugar DMEM medium containing 10% (v / v) fetal bovine serum, and place them at 37°C with a certain humidity of 5% CO 2 Cultured in a mixed gas (air 95%, v / v). After the miR-21-KD virus and its Null control virus were transfected, when the MSCs grew to 90% density, the medium was replaced with a medium containing 2% exosome-depleted serum, and the culture was continued for 48 hours before collection The supernatant was extracted with an exosome extraction kit to obtain exosome. Observe the obtained exosome under the electron microscope, the results are detailed in figure 1 .

[0027] Depend on figure 1 visible, figure 1 A and 1B are EnMSCs transfected with miR-21-KD virus and its Null control virus, after PCR for EnMSCs and its secreted exosome, compared with EnMSCs transfected with Null control virus, transfected with miR -21-KD EnMSCs down-regulated the l...

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Abstract

The invention relates to an application of miR-21 in preparing an EnMSCs (endometrium derived mesenchymal stem cells) paracrine effect regulator, and a culture method for regulating an EnMSCs paracrine effect. According to the application provided by the invention, it proves that with the down-regulated expression of the miR-21, an EnMSCs paracrine protective effect can be weakened. The invention provides the application of the miR-21 in preparing the EnMSCs paracrine effect regulator; and through miR-21-KD (miRNA-21 knockdown), the effects of an EnMSCs exosome on myocardium cell apoptosis resistance and angiogenesis of HUVECs (human umbilical vein endothelial cells) can be effectively inhibited, thus proving the effect of the miR-21 on regulating and controlling the EnMSCs.

Description

[0001] (1) Technical field [0002] The invention relates to the application of miR-21 in preparing a regulator of EnMSCs paracrine action, and a culture method for regulating EnMSCs paracrine action. [0003] (2) Background technology [0004] Nowadays, cardiovascular and cerebrovascular diseases have become the number one killer threatening human health. Although drug therapy and interventional therapy for the treatment of cardiovascular diseases have developed rapidly in recent years, and the prognosis of patients with ischemic cardiomyopathy has been significantly improved, drug therapy A major drawback of interventional therapy is the inability to repair necrotic myocardial tissue. At present, a large number of basic research and clinical experiments have confirmed that bone marrow mesenchymal stem cells (Bone marrow derived mesenchymalstem cells, BMMSCs) have a good effect on the treatment of cardiovascular diseases, especially its paracrine effect can effectively exert m...

Claims

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Application Information

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IPC IPC(8): C12N15/86C12N15/113C12N5/10C12Q1/02
CPCC12N5/0668C12N15/113G01N33/5026G01N33/5073
Inventor 王建安胡新央王侃
Owner ZHEJIANG UNIV
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