Rectal cancer radiotherapy resistance cell model and construction method therefor

Abstract

A construction method of a cell model is disclosed, by which a rectal cancer radiotherapy resistant cell strain is obtained in a manner of gradiently increasing radiation dose of X-ray, wherein the cell strain is sourced from an SW480 cell and the resistance of the resistant cell under high radiation dose and low radiation dose are both higher than those of the parent SW480 cell strain.

Description

technical field [0001] The invention belongs to the field of cells, in particular to the construction of cell models. Background technique [0002] In recent years, with the continuous improvement of people's living standards, the incidence of rectal cancer has increased year by year. Due to the special anatomical location of the rectum, early tumors are not easy to be found, and most patients are already in the middle and advanced stages when they seek treatment. These patients have a high recurrence rate after simple surgical resection, and radiotherapy is an important local treatment. The combination with surgery can significantly reduce the local recurrence rate and improve the long-term survival of patients. However, in the course of clinical treatment, we also observed that not all rectal cancer patients are sensitive to radiotherapy. The heterogeneity of rectal cancer cells’ response to X-rays affects the clinical application and curative effect of radiotherapy. The...

AI Technical Summary

Problems solved by technology

Among them, the "low-dose long-range induction method" usually repeatedly irradiates tumor cells with a dose of 2Gy for about 30 times to induce radiotherapy-resistant cell subsets in tumor cells. This process is time-consuming and requires repeated treatment of cells, increasing the The probability of contamination is reduced; and the "high-dose short-range screening method" is to increase the ratio of radioresistant cell subpopulations in tumor cells through single or several sublethal doses of irradiation, and then screen out radioresistant subpopulations of cells. In the method, a series of dose points must be used to screen out the dose that causes sublethal cell damage. During the screening process, the cells are easily deformed and die, and the radiobiological parameters of the established cells cannot represent the radioresistance of clinically recurrent cancer.