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Method for preparing alga feed by two-step fermentation

A two-step fermentation and seaweed technology, applied in animal feed, animal feed, application, etc., can solve the problems of waste, difficult digestion and absorption of animals, etc., and achieve the effect of high survival rate

Inactive Publication Date: 2017-02-01
福建省新闽科生物科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Similarly, seaweed has a great potential to be used as feed raw material and feed additive, and it will be a better feed resource, but seaweed contains a variety of anti-nutritional components, such as cellulose, sugar gum, xylan, etc. Non-starch polysaccharides are not easy for animals to digest and absorb, which indirectly causes waste, so how to improve the utilization rate of seaweed is an urgent problem to be solved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for preparing seaweed feed by two-step fermentation, the operation steps are:

[0019] Step 1. Washing and desalting with water

[0020] After the seaweed is crushed into a size of about 0.2 cm, the weight ratio of seaweed: water is 1:4, and the seaweed is washed with water, and the water is filtered to obtain a filter residue.

[0021] Step 2: Preparation of various seed liquids for seaweed fermentation

[0022] 1) Preparation of Bacillus licheniformis seed solution

[0023] Inoculate the liquid culture medium with slanted bacterial lawn, the medium formula is: 2 wt % seaweed, 1 wt % peptone, 0.3 wt % beef extract, 0.5 wt % sodium chloride, initial pH 7.0, 32 ° C, 200 rpm culture 20 hours.

[0024] 2) Preparation of Saccharomyces cerevisiae seed solution

[0025] The liquid medium was inoculated with slant bacterial lawn, the medium formula was: 3 wt% glucose, 0.5 wt% peptone, 0.1 wt% potassium dihydrogen phosphate, 0.1 wt% magnesium sulfate, 0.05 wt% pota...

Embodiment 2

[0037] A method for preparing seaweed feed by two-step fermentation, the operation steps are:

[0038] Step 1. Washing and desalting with water

[0039] After the seaweed is crushed into a size of about 0.2 cm, the weight ratio of seaweed: water is 1:5, and the seaweed is washed with water, and the water is filtered to obtain a filter residue.

[0040] Step 2: Preparation of various seed liquids for seaweed fermentation

[0041] The preparation methods of various seed solutions are as described in Example 1.

[0042] Step 3, the preparation of seaweed solid medium

[0043] Mix the filtered seaweed, bean dregs, and bran in a weight ratio of 10:2.5:1, and add 1 wt% glucose, 1 wt% ammonium sulfate, and 1 wt% phosphoric acid according to the weight ratio of the solid medium dry material Potassium dihydrogen, finally adjust the water content to 33 v / w%.

[0044] Step 4: Inoculate the seaweed culture medium with the compound bacteria liquid for fermentation

[0045] Both Bacill...

Embodiment 3

[0049] A method for preparing seaweed feed by two-step fermentation, the operation steps are:

[0050] Step 1. Washing and desalting with water

[0051] After the seaweed is crushed into a size of about 0.2 cm, the weight ratio of seaweed:water is 1:6, the seaweed is washed with water, and the water is filtered to obtain a filter residue.

[0052] Step 2: Preparation of various seed liquids for seaweed fermentation

[0053] The preparation methods of various seed solutions are as described in Example 1.

[0054] Step 3, the preparation of seaweed solid medium

[0055] Mix the filtered seaweed, bean dregs, and bran in a weight ratio of 7.5:2:1, and add 0.8 wt% glucose, 0.8 wt% ammonium sulfate, and 0.8 wt% phosphoric acid according to the weight ratio of the solid medium dry material Potassium dihydrogen, finally adjust the water content to 48v / w %.

[0056] Step 4: Inoculate the seaweed culture medium with the compound bacteria liquid for fermentation

[0057] Both Bacillus...

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Abstract

The invention relates to a method for preparing an alga feed by two-step fermentation. The method comprises the following steps: pulverizing algae into particles with the diameter of 0.2cm or so, carrying out primary water washing for desalting, mixing the algae with bean dregs and bran, regulating the initial water content to 28-50%, respectively inoculating Bacillus licheniformis, Saccharomyces cerevisiae, Lactobacillus acidophilus and Enterococcus faecium, fermenting, and drying to obtain the finished product. The prepared alga fermented feed has the nutritive value of the algae; the alga macromolecules in the feed are degraded to some degree, so that the feed can be absorbed by animals more easily; the feed contains certain quantities of Bacillus licheniformis and metabolites of the strains; especially, the lactein has the function of probiotics; and therefore, the feed can keep the microecological balance of the livestock / poultry intestinal tracts and enhance the immunity.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing seaweed feed through two-step fermentation. Background technique [0002] Seaweed is a nutrient-rich marine biological resource. It is an autotrophic plant capable of photosynthesis in the ocean. According to the different colors of its algae, it can be divided into red algae, green algae, brown algae, cyanobacteria, and golden algae. a variety of. The seaweed selected in the present invention is called Sargassum, which is a kind of brown algae, and its protein content is 8%-12%, which can reach 17%. More than 30%, containing a variety of vitamins, minerals, and some special ingredients, such as iodide, polyphenol compounds, sterol compounds and other active substances that promote animal growth. Studies have shown that seaweed has lipid-lowering, cholesterol-lowering, anti-cancer and anti-tumor effects, and is widely used in food industry, medici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12
Inventor 吴焜时祥柱姚丽梅连建芸况应谷陈炳钿
Owner 福建省新闽科生物科技开发有限公司
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